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THE PLANT CELL, Vol 1, Issue 1 15-24, Copyright © 1989 by American Society of Plant Biologists

RESEARCH ARTICLES

Isolation of Tissue-Specific cDNAs from Tomato Pistils

C. S. Gasser, K. A. Budelier, A. G. Smith, D. M. Shah and R. T. Fraley
Plant Molecular Biology, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, Missouri 63198

We have used a differential plaque hybridization screening procedure to isolate cDNA clones for genes that show elevated or exclusive expression in tomato pistils. Clones that showed maximal expression in immature pistils (premeiotic to early meiosis) and mature pistils (at anthesis) were isolated. Of nine clones that were characterized, four were found also to express at some stage of anther development. In situ hybridization experiments showed that expression of the genes we have identified is very tightly regulated both spatially and temporally within the pistil. One gene was identified that is expressed in the pistil only in the transmitting tissue of the style. A second gene was found to express exclusively in two to three cell layers of the ovules for a period of less than eight days.


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Copyright © 1989 by the American Society of Plant Biologists