Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gasser, C. S.
Right arrow Articles by Fraley, R. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gasser, C. S.
Right arrow Articles by Fraley, R. T.
Agricola
Right arrow Articles by Gasser, C. S.
Right arrow Articles by Fraley, R. T.

THE PLANT CELL, Vol 1, Issue 1 15-24, Copyright © 1989 by American Society of Plant Biologists

RESEARCH ARTICLES

Isolation of Tissue-Specific cDNAs from Tomato Pistils

C. S. Gasser, K. A. Budelier, A. G. Smith, D. M. Shah and R. T. Fraley
Plant Molecular Biology, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, Missouri 63198

We have used a differential plaque hybridization screening procedure to isolate cDNA clones for genes that show elevated or exclusive expression in tomato pistils. Clones that showed maximal expression in immature pistils (premeiotic to early meiosis) and mature pistils (at anthesis) were isolated. Of nine clones that were characterized, four were found also to express at some stage of anther development. In situ hybridization experiments showed that expression of the genes we have identified is very tightly regulated both spatially and temporally within the pistil. One gene was identified that is expressed in the pistil only in the transmitting tissue of the style. A second gene was found to express exclusively in two to three cell layers of the ovules for a period of less than eight days.


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Furumoto, S. Hata, and K. Izui
Isolation and Characterization of cDNAs for Differentially Accumulated Transcripts between Mesophyll Cells and Bundle Sheath Strands of Maize Leaves
Plant Cell Physiol., November 1, 2000; 41(11): 1200 - 1209.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. A. Hauser, J. Q. He, S. O. Park, and C. S. Gasser
TSO1 is a novel protein that modulates cytokinesis and cell expansion in Arabidopsis
Development, May 15, 2000; 127(10): 2219 - 2226.
[Abstract] [PDF]

Home page
 ScienceHome page
C. S. GASSER and R. T. FRALEY
Genetically Engineering Plants for Crop Improvement
Science, June 16, 1989; 244(4910): 1293 - 1299.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1989 by the American Society of Plant Biologists