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THE PLANT CELL, Vol 1, Issue 10 1011-1023, Copyright © 1989 by American Society of Plant Biologists

RESEARCH ARTICLES

Changes in the Zein Composition of Protein Bodies during Maize Endosperm Development

C. R. Lending and B. A. Larkins
Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907

Zeins, the seed storage proteins of maize, are synthesized during endosperm development by membrane-bound polyribosomes and transported into the lumen of the endoplasmic reticulum, where they assemble into protein bodies. To better understand the distribution of the various zeins throughout the endosperm, and within protein bodies, we used immunolocalization techniques with light and electron microscopy to study endosperm tissue at 14 days and 18 days after pollination. Protein bodies increase in size with distance from the aleurone layer of the developing endosperm; this reflects a process of cell maturation. The protein bodies within the subaleurone cell layer are the smallest and contain little or no [alpha]-zein; [beta]-zein and [gamma]-zein are distributed throughout these small protein bodies. The protein bodies in cells farther away from the aleurone layer are progressively larger, and immunostaining for [alpha]-zein occurs over locules in the central region of these protein bodies. In the interior of the largest protein bodies, the locules of [alpha]-zein are fused. Concomitant with the appearance of [alpha]-zein in the central regions of the protein bodies, most of the [beta]- and [gamma]-zeins become peripheral. These observations are consistent with a model in which specific zeins interact to assemble the storage proteins into a protein body.


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