Plant Cell Huazhong Agricultural University
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (122)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lam, E.
Right arrow Articles by Chua, N. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lam, E.
Right arrow Articles by Chua, N. H.
Agricola
Right arrow Articles by Lam, E.
Right arrow Articles by Chua, N. H.

THE PLANT CELL, Vol 1, Issue 12 1147-1156, Copyright © 1989 by American Society of Plant Biologists

RESEARCH ARTICLES

ASF-2: A Factor That Binds to the Cauliflower Mosaic Virus 35S Promoter and a Conserved GATA Motif in Cab Promoters

E. Lam and N. H. Chua
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021-6399

We have used nuclear extracts prepared from tobacco leaf tissue to characterize a factor binding site, designated as-2 (activating sequence-2), at the -100 region of the cauliflower mosaic virus 35S promoter. The activity of this factor, called ASF-2 (activating sequence factor-2), is not detected in tobacco root extracts. as-2 includes two GT motifs with sequence homology to the SV40 enhancer core A element and the Box II element of pea rbcS. Nevertheless, oligomers of these sequence elements do not compete for ASF-2 binding in gel retardation assays, indicating that the GT motifs may not be involved. Methylation interference studies identify two guanines (G93 and G98) that are required for interaction with ASF-2. Sequences surrounding these two critical guanines display homologies to a GATA repeat conserved among several light-responsive promoters. One such sequence from a petunia Cab promoter is able to compete with as-2 for factor binding. In transgenic plants, a tetramer of as-2 is able to confer leaf expression when fused 5[prime] to the -90 derivative of the 35S promoter. The expression is not dependent on light and, thus, the as-2 tetramer does not function as a light-responsive element in this context. Histochemical localization of the reporter gene product suggests that the as-2 tetramer directs expression in trichomes, vascular elements, and epidermal and mesophyll cells.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Okada, K. Okada, K. Miyamoto, J. Koga, N. Shibuya, H. Nojiri, and H. Yamane
OsTGAP1, a bZIP Transcription Factor, Coordinately Regulates the Inductive Production of Diterpenoid Phytoalexins in Rice
J. Biol. Chem., September 25, 2009; 284(39): 26510 - 26518.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Desai and J. Hu
Light Induces Peroxisome Proliferation in Arabidopsis Seedlings through the Photoreceptor Phytochrome A, the Transcription Factor HY5 HOMOLOG, and the Peroxisomal Protein PEROXIN11b
Plant Physiology, March 1, 2008; 146(3): 1117 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. de Dios Barajas-Lopez, A. J. Serrato, A. Olmedilla, A. Chueca, and M. Sahrawy
Localization in Roots and Flowers of Pea Chloroplastic Thioredoxin f and Thioredoxin m Proteins Reveals New Roles in Nonphotosynthetic Organs
Plant Physiology, November 1, 2007; 145(3): 946 - 960.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. W. Manfield, P. F. Devlin, C.-H. Jen, D. R. Westhead, and P. M. Gilmartin
Conservation, Convergence, and Divergence of Light-Responsive, Circadian-Regulated, and Tissue-Specific Expression Patterns during Evolution of the Arabidopsis GATA Gene Family
Plant Physiology, February 1, 2007; 143(2): 941 - 958.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Qi, Y. Zhang, and T. Chai
Characterization of a Novel Plant Promoter Specifically Induced by Heavy Metal and Identification of the Promoter Regions Conferring Heavy Metal Responsiveness
Plant Physiology, January 1, 2007; 143(1): 50 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Feng, F. An, S. Zhang, Z. Ji, H.-Q. Ling, and J. Zuo
Light-Regulated, Tissue-Specific, and Cell Differentiation-Specific Expression of the Arabidopsis Fe(III)-Chelate Reductase Gene AtFRO6
Plant Physiology, April 1, 2006; 140(4): 1345 - 1354.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
U. S. Lea, M.-T. Leydecker, I. Quillere, C. Meyer, and C. Lillo
Posttranslational Regulation of Nitrate Reductase Strongly Affects the Levels of Free Amino Acids and Nitrate, whereas Transcriptional Regulation Has Only Minor Influence
Plant Physiology, March 1, 2006; 140(3): 1085 - 1094.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Aksamit, A. Korobczak, J. Skala, M. Lukaszewicz, and J. Szopa
The 14-3-3 Gene Expression Specificity in Response to Stress is Promoter-Dependent
Plant Cell Physiol., October 1, 2005; 46(10): 1635 - 1645.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Leterrier, F. J. Corpas, J. B. Barroso, L. M. Sandalio, and L. A. del Rio
Peroxisomal Monodehydroascorbate Reductase. Genomic Clone Characterization and Functional Analysis under Environmental Stress Conditions
Plant Physiology, August 1, 2005; 138(4): 2111 - 2123.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Agius, I. Amaya, M. A. Botella, and V. Valpuesta
Functional analysis of homologous and heterologous promoters in strawberry fruits using transient expression
J. Exp. Bot., January 1, 2005; 56(409): 37 - 46.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Bharti, P. von Koskull-Doring, S. Bharti, P. Kumar, A. Tintschl-Korbitzer, E. Treuter, and L. Nover
Tomato Heat Stress Transcription Factor HsfB1 Represents a Novel Type of General Transcription Coactivator with a Histone-Like Motif Interacting with the Plant CREB Binding Protein Ortholog HAC1
PLANT CELL, June 1, 2004; 16(6): 1521 - 1535.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. C. Reyes, M. I. Muro-Pastor, and F. J. Florencio
The GATA Family of Transcription Factors in Arabidopsis and Rice
Plant Physiology, April 1, 2004; 134(4): 1718 - 1732.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. H. Yang, H. Yu, and C. J. Goh
Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter
J. Exp. Bot., September 1, 2002; 53(376): 1899 - 1907.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Yamagata, K. Yonesu, A. Hirata, and Y. Aizono
TGTCACA Motif Is a Novel cis-Regulatory Enhancer Element Involved in Fruit-specific Expression of the cucumisin Gene
J. Biol. Chem., March 22, 2002; 277(13): 11582 - 11590.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. K. Deyholos and L. E. Sieburth
Separable Whorl-Specific Expression and Negative Regulation by Enhancer Elements within the AGAMOUS Second Intron
PLANT CELL, October 1, 2000; 12(10): 1799 - 1810.
[Abstract] [Full Text]

Home page
 ScienceHome page
P. N. Benfey and N.-H. Chua
The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants
Science, November 16, 1990; 250(4983): 959 - 966.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1989 by the American Society of Plant Biologists