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THE PLANT CELL, Vol 1, Issue 2 173-179, Copyright © 1989 by American Society of Plant Biologists
Characterization of a Pollen-Specific cDNA Clone from Zea mays and Its Expression
D. D. Hanson, D. A. Hamilton, J. L. Travis, D. M. Bashe and J. P. Mascarenhas
Department of Biological Sciences, State University of New York at Albany, Albany, New York 12222
A pollen-specific cDNA clone, Zmc13, has been isolated from a cDNA library
constructed to poly(A) RNA from mature maize pollen. The cDNA as shown by
primer extension analysis is a full-length copy of the mRNA. The cDNA has
been sequenced and is 929 nucleotides in length plus a 47-nucleotide
poly(A) tail. Putative polyadenylation signals are identifiable in the
3[prime]-nontranslated region. The mRNA codes for a predicted polypeptide
containing 170 amino acid residues and with a molecular mass of 18.3
kilodaltons. The hydropathy profile suggests a possible signal sequence on
the amino terminus. A comparison of the nucleotide and deduced amino acid
sequence with sequences in data banks has not shown homology to known
molecules. In situ hybridizations using RNA probes show that the mRNA is
located in the cytoplasm of the vegetative cell of the pollen grain and
after germination is distributed throughout the pollen tube cytoplasm.
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