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THE PLANT CELL, Vol 1, Issue 2 191-200, Copyright © 1989 by American Society of Plant Biologists
Actin Localization during Fucus Embryogenesis
D. L. Kropf, S. K. Berge and R. S. Quatrano
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331
Embryogenesis in the Fucales serves as a model system for studying the
acquisition of cellular and developmental polarity. Fertilized eggs bear no
asymmetry, yet within 16 hours, a developmental axis is formed and the
unicellular zygote germinates in accordance with this axis. Microfilaments
(actin) play a crucial role in establishing the axis as evidenced by the
inhibitory effects of cytochalasins on axis fixation. The cellular content
of actin was determined by immunoblot, whereas the localization of F-actin
was investigated using the fluorescent probe rhodamine phalloidin. Three
isoforms of actin were detected in constant amounts at all developmental
stages. Actin networks were found to be distributed uniformly in eggs and
zygotes through the period of early zygote development when the polar axis
was formed. However, as the polar axis became irreversibly fixed in space,
actin was localized at the presumptive germination site by a
cytochalasin-sensitive process. This correlation supports the proposal that
actin networks play a critical role in axis fixation, and is consistent
with our hypothesis that this process involves stabilization of membrane
components by transmembrane bridges from the cell wall to the microfilament
cytoskeleton.
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