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THE PLANT CELL, Vol 1, Issue 3 285-291, Copyright © 1989 by American Society of Plant Biologists
Constitutive Expression of Pathogenesis-Related Proteins PR-1, GRP, and PR-S in Tobacco Has No Effect on Virus Infection
HJM. Linthorst, RLJ. Meuwissen, S. Kauffmann and J. F. Bol
Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
Samsun NN tobacco cells were transformed with chimeric genes for
pathogenesis-related (PR) proteins derived from genomic (PR-1a, GRP) or
cDNA (PR-S) clones under the transcriptional control of the cauliflower
mosaic virus 35S promoter. Regenerated plants were assayed by RNA and
protein gel blotting, and plants showing high specific expression of the
inserted genes were selected for self-pollination and seed formation.
Inspection of second generation transformants showed that constitutive
expression of PR-1a, GRP, and PR-S in tobacco in general does not have an
effect on the phenotypic appearance of the plants or the expression of
other endogenous PR genes. Furthermore, constitutive expression of the
above genes does not affect the susceptibility of the plants to infection
with tobacco mosaic virus or alfalfa mosaic virus.
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