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THE PLANT CELL, Vol 1, Issue 3 293-300, Copyright © 1989 by American Society of Plant Biologists
Coordinate Expression of Transcriptionally Regulated Isocitrate Lyase and Malate Synthase Genes in Brassica napus L
L. Comai, R. A. Dietrich, D. J. Maslyar, C. S. Baden and J. J. Harada
Department of Botany, University of California, Davis, California 95616
We have analyzed the temporal and spatial expression of genes encoding the
glyoxylate cycle enzymes isocitrate lyase and malate synthase in Brassica
napus L. to determine whether they are coordinately expressed. Both enzymes
participate in reactions associated with lipid mobilization in oilseed
plant seedlings and are sequestered in a specialized organelle, the
glyoxysome. We have identified an isocitrate lyase cDNA clone containing
the complete protein coding region. RNA blot and in situ hybridization
studies with isocitrate lyase and malate synthase cDNA clones from B. napus
showed that the genes exhibit similar expression patterns. The mRNAs begin
to accumulate during late embryogeny, reach maximal levels in seedling
cotyledons, are not detected at significant amounts in leaves, and are
distributed similarly in cotyledons and axes of seedlings. Furthermore,
transcription studies with isolated nuclei indicate that the genes are
controlled primarily although not exclusively at the transcriptional level.
We conclude that glyoxysome biogenesis is regulated in part through the
coordinate expression of isocitrate lyase and malate synthase genes.
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