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THE PLANT CELL, Vol 1, Issue 4 391-401, Copyright © 1989 by American Society of Plant Biologists
Two Glutamine Synthetase Genes from Phaseolus vulgaris L. Display Contrasting Developmental and Spatial Patterns of Expression in Transgenic Lotus corniculatus Plants
B. G. Forde, H. M. Day, J. F. Turton, S. Wen-jun, J. V. Cullimore and J. E. Oliver
Biochemistry Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, United Kingdom
The gln-[gamma] gene, which specifies the [gamma] subunit of glutamine
synthetase in Phaseolus vulgaris L., has been isolated and the regulatory
properties of its promoter region analyzed in transgenic Lotus corniculatus
plants. A 2-kilobase fragment from the 5[prime]-flanking region of
gln-[gamma] conferred a strongly nodule-enhanced pattern of expression on
the [beta]-glucuronidase reporter gene. Parallel studies on the promoter of
another glutamine synthetase gene (gln-[beta]) showed that a 1.7-kilobase
fragment directed 20-fold to 140-fold higher levels of [beta]-glucuronidase
expression in roots than in shoots. Histochemical localization of
[beta]-glucuronidase activity in nodules of the transgenic plants indicated
that the chimeric gln-[gamma] gene was expressed specifically in the
rhizobially infected cells; expression of the gln-[beta] construct was
detected in both cortical and infected regions of young nodules, and became
restricted to the vascular tissue as the nodule matured. We conclude that
gln-[beta] and gln-[gamma] genes are differentially expressed both
temporally and spatially in plant development and that the cis-acting
regulatory elements responsible for conferring these contrasting expression
patterns are located within a 2-kilobase region upstream of their coding
sequences.
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