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THE PLANT CELL, Vol 1, Issue 9 901-911, Copyright © 1989 by American Society of Plant Biologists
Molecular Characterization of a Zygote Wall Protein: An Extensin-Like Molecule in Chlamydomonas reinhardtii
J. P. Woessner and U. W. Goodenough
Department of Biology, Washington University, St. Louis, Missouri 63130
The green alga Chlamydomonas reinhardtii elaborates two biochemically and
morphologically distinct cell walls during its life cycle: one surrounds
the vegetative and gametic cell and the other encompasses the zygote.
Hydroxyproline-rich glycoproteins (HRGPs) constitute a major component of
both walls. We describe the isolation and characterization of a
zygote-specific gene encoding a wall HRGP. The derived amino acid sequence
of this algal HRGP is similar to those of higher plant extensins, rich in
proline and serine residues and possessing repeating amino acid motifs,
notably X(Pro)3 and (Ser-Pro)n. Antiserum against this zygote wall protein
detected common epitopes in several other zygote polypeptides, at least one
of which is also encoded by a zygote-specific gene. We conclude that there
is one set of HRGP wall genes expressed only in zygotes and another set
that is specific to vegetative and gametic cells.
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