Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (84)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Knoblauch, M.
Right arrow Articles by van Bel, A. J. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Knoblauch, M.
Right arrow Articles by van Bel, A. J. E.
Agricola
Right arrow Articles by Knoblauch, M.
Right arrow Articles by van Bel, A. J. E.
THE PLANT CELL ONLINE, Vol. 10, 35-50, Copyright © 1998, American Society of Plant Physiologists

Sieve Tubes in Action

Michael Knoblaucha and Aart J. E. van Bela
a Institute for General Botany and Plant Physiology, Justus-Liebig-University, Senckenbergstrasse 17, 35390 Giessen, Germany

Correspondence to: Aart J. E. van Bel, aart.v.bel{at}bot1.bio.uni-giessen.de (E-mail), 49-641-99-35119 (fax).

A method was designed for in vivo observation of sieve element/companion complexes by using confocal laser scanning microscopy. A leaf attached to an intact fava bean plant was mounted upside down on the stage of a confocal microscope. Two shallow paradermal cortical cuts were made in the major vein. The basal cortical window allowed us to observe the phloem intact. The apical window at 3 cm from the site of observation was used to apply phloem-mobile fluorochromes, which identified living sieve elements at the observation site. In intact sieve tubes, the sieve plates did not present a barrier to mass flow, because the translocation of fluorochromes appeared to be unhindered. Two major occlusion mechanisms were distinguished. In response to intense laser light, the parietal proteins detached from the plasma membrane and formed a network of minute strands and clustered material that aggregated and pressed against the sieve plate. In response to mechanical damage, the evenly distributed P plastids exploded, giving rise to the formation of a massive plug against the sieve plate. In case of mechanical damage, the parietal proteins transformed into elastic threads (strands) that extended throughout the sieve element lumen. Our observations cover the phenomena encountered in previous microscopic and electron microscopic studies and provide a temporal disentanglement of the events giving rise to the confusing mass of structures observed thus far.




This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
T. Will, S. R. Kornemann, A. C. U. Furch, W. F. Tjallingii, and A. J. E. van Bel
Aphid watery saliva counteracts sieve-tube occlusion: a universal phenomenon?
J. Exp. Biol., October 15, 2009; 212(20): 3305 - 3312.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. C.U. Furch, A. J.E. van Bel, M. D. Fricker, H. H. Felle, M. Fuchs, and J. B. Hafke
Sieve Element Ca2+ Channels as Relay Stations between Remote Stimuli and Sieve Tube Occlusion in Vicia faba
PLANT CELL, July 1, 2009; 21(7): 2118 - 2132.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. M. Christensen, C. Faulkner, and K. Oparka
Evidence for Unidirectional Flow through Plasmodesmata
Plant Physiology, May 1, 2009; 150(1): 96 - 104.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M.-K. Lin, Y.-J. Lee, T. J. Lough, B. S. Phinney, and W. J. Lucas
Analysis of the Pumpkin Phloem Proteome Provides Insights into Angiosperm Sieve Tube Function
Mol. Cell. Proteomics, February 1, 2009; 8(2): 343 - 356.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. V. Thompson and S. M. Wolniak
A Plasma Membrane-Anchored Fluorescent Protein Fusion Illuminates Sieve Element Plasma Membranes in Arabidopsis and Tobacco
Plant Physiology, April 1, 2008; 146(4): 1599 - 1610.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. A. Glaring, A. Zygadlo, D. Thorneycroft, A. Schulz, S. M. Smith, A. Blennow, and L. Baunsgaard
An extra-plastidial {alpha}-glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation
J. Exp. Bot., November 17, 2007; (2007) erm249v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. C. U. Furch, J. B. Hafke, A. Schulz, and A. J. E. van Bel
Ca2+-mediated remote control of reversible sieve tube occlusion in Vicia faba
J. Exp. Bot., August 1, 2007; 58(11): 2827 - 2838.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. S. Peters, A. J. E. van Bel, and M. Knoblauch
The geometry of the forisome-sieve element-sieve plate complex in the phloem of Vicia faba L. leaflets
J. Exp. Bot., September 1, 2006; 57(12): 3091 - 3098.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C Doering-Saad, H. Newbury, C. Couldridge, J. Bale, and J Pritchard
A phloem-enriched cDNA library from Ricinus: insights into phloem function
J. Exp. Bot., September 1, 2006; 57(12): 3183 - 3193.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Will and A. J. E. van Bel
Physical and chemical interactions between aphids and plants
J. Exp. Bot., March 1, 2006; 57(4): 729 - 737.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. B. Hafke, J.-K. van Amerongen, F. Kelling, A. C.U. Furch, F. Gaupels, and A. J.E. van Bel
Thermodynamic Battle for Photosynthate Acquisition between Sieve Tubes and Adjoining Parenchyma in Transport Phloem
Plant Physiology, July 1, 2005; 138(3): 1527 - 1537.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G.-L. Wu, X.-Y. Zhang, L.-Y. Zhang, Q.-H. Pan, Y.-Y. Shen, and D.-P. Zhang
Phloem Unloading in Developing Walnut Fruit is Symplasmic in the Seed Pericarp and Apoplasmic in the Fleshy Pericarp
Plant Cell Physiol., October 15, 2004; 45(10): 1461 - 1470.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B.-C. Yoo, F. Kragler, E. Varkonyi-Gasic, V. Haywood, S. Archer-Evans, Y. M. Lee, T. J. Lough, and W. J. Lucas
A Systemic Small RNA Signaling System in Plants
PLANT CELL, August 1, 2004; 16(8): 1979 - 2000.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L.-Y. Zhang, Y.-B. Peng, S. Pelleschi-Travier, Y. Fan, Y.-F. Lu, Y.-M. Lu, X.-P. Gao, Y.-Y. Shen, S. Delrot, and D.-P. Zhang
Evidence for Apoplasmic Phloem Unloading in Developing Apple Fruit
Plant Physiology, May 1, 2004; 135(1): 574 - 586.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. A. Bird, V. R. Franceschi, and P. J. Facchini
A Tale of Three Cell Types: Alkaloid Biosynthesis Is Localized to Sieve Elements in Opium Poppy
PLANT CELL, November 1, 2003; 15(11): 2626 - 2635.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Dinant, A. M. Clark, Y. Zhu, F. Vilaine, J.-C. Palauqui, C. Kusiak, and G. A. Thompson
Diversity of the Superfamily of Phloem Lectins (Phloem Protein 2) in Angiosperms
Plant Physiology, January 1, 2003; 131(1): 114 - 128.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. I. Prokhnevsky, V. V. Peremyslov, A. J. Napuli, and V. V. Dolja
Interaction between Long-Distance Transport Factor and Hsp70-Related Movement Protein of Beet Yellows Virus
J. Virol., November 1, 2002; 76(21): 11003 - 11011.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Itaya, F. Ma, Y. Qi, Y. Matsuda, Y. Zhu, G. Liang, and B. Ding
Plasmodesma-Mediated Selective Protein Traffic between "Symplasmically Isolated" Cells Probed by a Viral Movement Protein
PLANT CELL, September 1, 2002; 14(9): 2071 - 2083.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B.-C. Yoo, J.-Y. Lee, and W. J. Lucas
Analysis of the Complexity of Protein Kinases within the Phloem Sieve Tube System. CHARACTERIZATION OF CUCURBITA MAXIMA CALMODULIN-LIKE DOMAIN PROTEIN KINASE 1
J. Biol. Chem., May 3, 2002; 277(18): 15325 - 15332.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Rouhier, E. Gelhaye, P.-E. Sautiere, A. Brun, P. Laurent, D. Tagu, J. Gerard, E. de Fay, Y. Meyer, and J.-P. Jacquot
Isolation and Characterization of a New Peroxiredoxin from Poplar Sieve Tubes That Uses Either Glutaredoxin or Thioredoxin as a Proton Donor
Plant Physiology, November 1, 2001; 127(3): 1299 - 1309.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
A Calcium-Regulated Gatekeeper in Phloem Sieve Tubes
PLANT CELL, May 1, 2001; 13(5): 989 - 992.
[Full Text]

Home page
 Plant CellHome page
M. Knoblauch, W. S. Peters, K. Ehlers, and A. J. E. van Bel
Reversible Calcium-Regulated Stopcocks in Legume Sieve Tubes
PLANT CELL, May 1, 2001; 13(5): 1221 - 1230.
[Abstract] [Full Text]

Home page
 J Exp BotHome page
S. Haupt, K. J. Oparka, N. Sauer, and S. Neumann
Macromolecular trafficking between Nicotiana tabacum and the holoparasite Cuscuta reflexa
J. Exp. Bot., January 1, 2001; 52(354): 173 - 177.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. J. Oparka and R. Turgeon
Sieve Elements and Companion Cells—Traffic Control Centers of the Phloem
PLANT CELL, April 1, 1999; 11(4): 739 - 750.
[Full Text]

Home page
 Plant CellHome page
B. Golecki, A. Schulz, and G. A. Thompson
Translocation of Structural P Proteins in the Phloem
PLANT CELL, January 1, 1999; 11(1): 127 - 140.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1998 by the American Society of Plant Biologists