Plant Cell Applied BioSystems, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (48)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shiina, T.
Right arrow Articles by Maliga, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shiina, T.
Right arrow Articles by Maliga, P.
Agricola
Right arrow Articles by Shiina, T.
Right arrow Articles by Maliga, P.
Plant Cell, Vol. 10, 1713-1722, October 1998, Copyright © 1998, American Society of Plant Physiologists

rbcL Transcript Levels in Tobacco Plastids Are Independent of Light: Reduced Dark Transcription Rate Is Compensated by Increased mRNA Stability

Takashi Shiinaa, Lori Allisona, and Pal Maligaa
a Waksman Institute, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, New Jersey 08854-8020

Correspondence to: Pal Maliga, maliga{at}waksman.rutgers.edu (E-mail), 732-445-5735 (fax).

The plastid rbcL gene, encoding the large subunit of ribulose-1,5-bisphosphate carboxylase, in higher plants is transcribed from a {sigma}70 promoter by the eubacterial-type RNA polymerase. To identify regulatory elements outside of the rbcL -10/-35 promoter core, we constructed transplastomic tobacco plants with uidA reporter genes expressed from rbcL promoter derivatives. Promoter activity was characterized by measuring steady state levels of uidA mRNA on RNA gel blots and by measuring promoter strength in run-on transcription assays. We report here that the rbcL core promoter is sufficient to obtain wild-type rates of transcription. Furthermore, the rates of transcription were up to 10-fold higher in light-grown leaves than in dark-adapted plants. Although the rates of transcription were lower in the dark, rbcL mRNA accumulated to similar levels in light-grown and dark-adapted leaves. Accumulation of uidA mRNA from most rbcL promoter deletion derivatives directly reflected the relative rates of transcription: high in the light-grown and low in the dark-adapted leaves. However, uidA mRNA accumulated to high levels in a light-independent fashion as long as a segment encoding a stem–loop structure in the 5' untranslated region was included in the promoter construct. This finding indicates that lower rates of rbcL transcription in the dark are compensated by increased mRNA stability.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
V. T. Valkov, N. Scotti, S. Kahlau, D. MacLean, S. Grillo, J. C. Gray, R. Bock, and T. Cardi
Genome-Wide Analysis of Plastid Gene Expression in Potato Leaf Chloroplasts and Tuber Amyloplasts: Transcriptional and Posttranscriptional Control
Plant Physiology, August 1, 2009; 150(4): 2030 - 2044.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
J. J. Baecker, J. C. Sneddon, and M. J. Hollingsworth
Efficient translation in chloroplasts requires element(s) upstream of the putative ribosome binding site from atpI
Am. J. Botany, March 1, 2009; 96(3): 627 - 636.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. A. Mudd, S. Sullivan, M. F. Gisby, A. Mironov, C. S. Kwon, W.-I. Chung, and A. Day
A 125 kDa RNase E/G-like protein is present in plastids and is essential for chloroplast development and autotrophic growth in Arabidopsis
J. Exp. Bot., July 1, 2008; 59(10): 2597 - 2610.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. M. Whitney and R. E. Sharwood
Construction of a tobacco master line to improve Rubisco engineering in chloroplasts
J. Exp. Bot., May 1, 2008; 59(7): 1909 - 1921.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Patel and J. O. Berry
Rubisco gene expression in C4 plants
J. Exp. Bot., May 1, 2008; 59(7): 1625 - 1634.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Hirata, D. Yonekura, S. Yanagisawa, and K. Iba
Possible Involvement of the 5'-Flanking Region and the 5'UTR of Plastid accD Gene in NEP-Dependent Transcription
Plant Cell Physiol., February 15, 2004; 45(2): 176 - 186.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Hayashi, T. Shiina, N. Ishii, K. Iwai, Y. Ishizaki, K. Morikawa, and Y. Toyoshima
A Role of the -35 Element in the Initiation of Transcription at psbA Promoter in Tobacco Plastids
Plant Cell Physiol., March 15, 2003; 44(3): 334 - 341.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Y. Suzuki, P. Sriraman, Z. Svab, and P. Maliga
Unique Architecture of the Plastid Ribosomal RNA Operon Promoter Recognized by the Multisubunit RNA Polymerase in Tobacco and Other Higher Plants
PLANT CELL, January 1, 2003; 15(1): 195 - 205.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. E. Thum, M. Kim, D. A. Christopher, and J. E. Mullet
Cryptochrome 1, Cryptochrome 2, and Phytochrome A Co-Activate the Chloroplast psbD Blue Light-Responsive Promoter
PLANT CELL, December 1, 2001; 13(12): 2747 - 2760.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Chun, A. Kawakami, and D. A. Christopher
Phytochrome A Mediates Blue Light and UV-A-Dependent Chloroplast Gene Transcription in Green Leaves
Plant Physiology, April 1, 2001; 125(4): 1957 - 1966.
[Abstract] [Full Text]

Home page
 Nucleic Acids ResHome page
H. Kuroda and P. Maliga
Complementarity of the 16S rRNA penultimate stem with sequences downstream of the AUG destabilizes the plastid mRNAs
Nucleic Acids Res., February 15, 2001; 29(4): 970 - 975.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Baba, T. Nakano, K. Yamagishi, and S. Yoshida
Involvement of a Nuclear-Encoded Basic Helix-Loop-Helix Protein in Transcription of the Light-Responsive Promoter of psbD
Plant Physiology, February 1, 2001; 125(2): 595 - 603.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
H. Kuroda and P. Maliga
Sequences Downstream of the Translation Initiation Codon Are Important Determinants of Translation Efficiency in Chloroplasts
Plant Physiology, January 1, 2001; 125(1): 430 - 436.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S. D. Lahiri and L. A. Allison
Complementary Expression of Two Plastid-Localized sigma -Like Factors in Maize
Plant Physiology, July 1, 2000; 123(3): 883 - 894.
[Abstract] [Full Text]

Home page
 Plant CellHome page
A. B. Cahoon and M. P. Timko
yellow-in-the-dark Mutants of Chlamydomonas Lack the CHLL Subunit of Light-Independent Protochlorophyllide Reductase
PLANT CELL, April 1, 2000; 12(4): 559 - 568.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
G. Schuster, I. Lisitsky, and P. Klaff
Polyadenylation and Degradation of mRNA in the Chloroplast
Plant Physiology, August 1, 1999; 120(4): 937 - 944.
[Full Text]

Home page
 Plant CellHome page
J. Nickelsen, M. Fleischmann, E. Boudreau, M. Rahire, and J.-D. Rochaix
Identification of cis-Acting RNA Leader Elements Required for Chloroplast psbD Gene Expression in Chlamydomonas
PLANT CELL, May 1, 1999; 11(5): 957 - 970.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
V Willemsen, H Wolkenfelt, G de Vrieze, P Weisbeek, and B Scheres
The HOBBIT gene is required for formation of the root meristem in the Arabidopsis embryo
Development, January 2, 1998; 125(3): 521 - 531.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
M. Horlitz and P. Klaff
Gene-specific trans-Regulatory Functions of Magnesium for Chloroplast mRNA Stability in Higher Plants
J. Biol. Chem., November 3, 2000; 275(45): 35638 - 35645.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. J. McCormac, H. Litz, J. Wang, P. D. Gollnick, and J. O. Berry
Light-associated and Processing-dependent Protein Binding to 5' Regions of rbcL mRNA in the Chloroplasts of a C4 Plant
J. Biol. Chem., January 26, 2001; 276(5): 3476 - 3483.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1998 by the American Society of Plant Biologists