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Plant Cell, Vol. 10, 2005-2018, December 1998, Copyright © 1998, American Society of Plant Physiologists
Imbibition, but Not Release from Stratification, Sets the Circadian Clock in Arabidopsis Seedlings
Hai Hong Zhonga,
Janet E. Paintera,
Patrice A. Saloméa,
Martin Straumeb, and
C. Robertson McClunga
a Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755
b National Science Foundation Center for Biological Timing, Department of Internal Medicine, Division of Endocrinology and Metabolism, University of Virginia, Charlottesville, Virginia 22903
Correspondence to:
C. Robertson McClung, mcclung{at}dartmouth.edu (E-mail), 603-646-1347 (fax).
Circadian rhythms in the abundance of the CAT2 catalase mRNA were not seen in etiolated seedlings but developed upon illumination. These circadian oscillations were preceded by a rapid and transient induction of CAT2 mRNA abundance that varied strikingly according to the timing (circadian phase) of the onset of illumination. This variation oscillated with a circadian periodicity of ~28 hr, indicating that the circadian oscillator is running in etiolated seedlings and regulates (gates) the induction of CAT2 by light. Moreover, because we assayed populations of seedlings, we infer that the individual clocks among populations of etiolated seedlings were synchronized before the onset of illumination. What developmental or environmental signals synchronized the clocks among seedlings? Varying the phase of the onset of illumination relative to release from stratification failed to affect the acute induction of CAT2, indicating that the temperature step from 4 to 22°C associated with release from stratification did not reset the circadian clock. However, the acute induction of CAT2 mRNA varied with time after imbibition, demonstrating that imbibition provides a signal capable of resetting the circadian clock and of synchronizing the clocks among populations of seedlings.
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