Plant Cell email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (67)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Higashiyama, T.
Right arrow Articles by Kuroiwa, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Higashiyama, T.
Right arrow Articles by Kuroiwa, T.
Agricola
Right arrow Articles by Higashiyama, T.
Right arrow Articles by Kuroiwa, T.
Plant Cell, Vol. 10, 2019-2032, December 1998, Copyright © 1998, American Society of Plant Physiologists

Guidance in Vitro of the Pollen Tube to the Naked Embryo Sac of Torenia fournieri

Tetsuya Higashiyamaa, Haruko Kuroiwab, Shigeyuki Kawanoa, and Tsuneyoshi Kuroiwaa
a Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan
b Kyoritsu Women's Junior College, Kanda, Tokyo 101-0051, Japan

Correspondence to: Tetsuya Higashiyama, higashi{at}biol.s.u-tokyo.ac.jp (E-mail).

The precise guidance of the pollen tube to the embryo sac is critical to the successful sexual reproduction of flowering plants. We demonstrate here the guidance of the pollen tube to the embryo sac in vitro by using the naked embryo sac of Torenia fournieri, which protrudes from the micropyle of the ovule. We developed a medium for culture of both the ovule and the pollen tube of T. fournieri and cocultivated them in a thin layer of solid medium. Although pollen tubes that had germinated in vitro passed naked embryo sacs, some pollen tubes that grew semi–in vitro through a cut style arrived precisely at the site of entry into the embryo sac, namely, the filiform apparatus of the synergids. When pollen tubes were unable to enter the embryo sac, they continuously grew toward the same filiform apparatus, forming narrow coils. Pollen tubes selectively arrived at complete, unfertilized embryo sacs but did not arrive at those of heat-treated ovules or those with disrupted synergids. These results convincingly demonstrate that pollen tubes are specifically attracted to the region of the filiform apparatus of living synergids in vitro.




This article has been cited by other articles:


Home page
 DevelopmentHome page
A. Boisson-Dernier, S. Roy, K. Kritsas, M. A. Grobei, M. Jaciubek, J. I. Schroeder, and U. Grossniklaus
Disruption of the pollen-expressed FERONIA homologs ANXUR1 and ANXUR2 triggers pollen tube discharge
Development, October 1, 2009; 136(19): 3279 - 3288.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Wang, L. C. Boavida, M. Ron, and S. McCormick
Truncation of a Protein Disulfide Isomerase, PDIL2-1, Delays Embryo Sac Maturation and Disrupts Pollen Tube Guidance in Arabidopsis thaliana
PLANT CELL, December 1, 2008; 20(12): 3300 - 3311.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
N. Rotman, M. Gourgues, A.-E. Guitton, J.-E. Faure, and F. Berger
A Dialogue between the Sirene Pathway in Synergids and the Fertilization Independent Seed Pathway in the Central Cell Controls Male Gamete Release during Double Fertilization in Arabidopsis
Mol Plant, July 1, 2008; 1(4): 659 - 666.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J.-Z. Wu, Y. Lin, X.-L. Zhang, D.-W. Pang, and J. Zhao
IAA stimulates pollen tube growth and mediates the modification of its wall composition and structure in Torenia fournieri
J. Exp. Bot., June 1, 2008; 59(9): 2529 - 2543.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kubo, J. Abe, T. Oyamada, M. Ohnishi, H. Fukuzawa, Y. Matsuda, and T. Saito
Characterization of novel genes induced by sexual adhesion and gamete fusion and of their transcriptional regulation in Chlamydomonas reinhardtii
Plant Cell Physiol., June 1, 2008; 49(6): 981 - 993.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
L. L. Ge, H. Q. Tian, and S. D. Russell
Calcium function and distribution during fertilization in angiosperms
Am. J. Botany, June 1, 2007; 94(6): 1046 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. A. Long
Plant development: new models and approaches bring progress
Development, December 1, 2006; 133(23): 4609 - 4612.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Higashiyama, R. Inatsugi, S. Sakamoto, N. Sasaki, T. Mori, H. Kuroiwa, T. Nakada, H. Nozaki, T. Kuroiwa, and A. Nakano
Species Preferentiality of the Pollen Tube Attractant Derived from the Synergid Cell of Torenia fournieri
Plant Physiology, October 1, 2006; 142(2): 481 - 491.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. D. Kasahara, M. F. Portereiko, L. Sandaklie-Nikolova, D. S. Rabiger, and G. N. Drews
MYB98 Is Required for Pollen Tube Guidance and Synergid Cell Differentiation in Arabidopsis
PLANT CELL, November 1, 2005; 17(11): 2981 - 2992.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B. H.J. de Graaf, A. Y. Cheung, T. Andreyeva, K. Levasseur, M. Kieliszewski, and H.-m. Wu
Rab11 GTPase-Regulated Membrane Trafficking Is Crucial for Tip-Focused Pollen Tube Growth in Tobacco
PLANT CELL, September 1, 2005; 17(9): 2564 - 2579.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
P. GIBBS, M. B. BIANCHI, and N. TARODA RANGA
Effects of Self-, Chase and Mixed Self/Cross-pollinations on Pistil Longevity and Fruit Set in Ceiba Species (Bombacaceae) with Late-acting Self-incompatibility
Ann. Bot., August 1, 2004; 94(2): 305 - 310.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Yadegari and G. N. Drews
Female Gametophyte Development
PLANT CELL, June 1, 2004; 16(suppl_1): S133 - S141.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Kim, J.-C. Mollet, J. Dong, K. Zhang, S.-Y. Park, and E. M. Lord
From The Cover: Chemocyanin, a small basic protein from the lily stigma, induces pollen tube chemotropism
PNAS, December 23, 2003; 100(26): 16125 - 16130.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
N. Huck, J. M. Moore, M. Federer, and U. Grossniklaus
The Arabidopsis mutant feronia disrupts the female gametophytic control of pollen tube reception
Development, May 15, 2003; 130(10): 2149 - 2159.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. M. Lord
Adhesion and guidance in compatible pollination
J. Exp. Bot., January 1, 2003; 54(380): 47 - 54.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
T. Higashiyama, S. Yabe, N. Sasaki, Y. Nishimura, S.-y. Miyagishima, H. Kuroiwa, and T. Kuroiwa
Pollen Tube Attraction by the Synergid Cell
Science, August 24, 2001; 293(5534): 1480 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Shimizu and K Okada
Attractive and repulsive interactions between female and male gametophytes in Arabidopsis pollen tube guidance
Development, January 10, 2000; 127(20): 4511 - 4518.
[Abstract] [PDF]

Home page
 Plant Physiol.Home page
T. Higashiyama, H. Kuroiwa, S. Kawano, and T. Kuroiwa
Explosive Discharge of Pollen Tube Contents in Torenia fournieri
Plant Physiology, January 1, 2000; 122(1): 11 - 14.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1998 by the American Society of Plant Biologists