Plant Cell Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (99)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhong, R.
Right arrow Articles by Ye, Z.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhong, R.
Right arrow Articles by Ye, Z.-H.
Agricola
Right arrow Articles by Zhong, R.
Right arrow Articles by Ye, Z.-H.
Plant Cell, Vol. 10, 2033-2046, December 1998, Copyright © 1998, American Society of Plant Physiologists

Dual Methylation Pathways in Lignin Biosynthesis

Ruiqin Zhonga, W. Herbert Morrison IIIb, Jonathan Negrelc, and Zheng-Hua Yea
a Department of Botany, University of Georgia, Athens, Georgia 30602
b Richard B. Russell Agriculture Research Center, U.S. Department of Agriculture, Agricultural Research Service, P.O. Box 5677, Athens, Georgia 30604
c Laboratoire de Physiopathologie Végétale, INRA, BV1540, 21034 Di1jon, France

Correspondence to: Zheng-Hua Ye, ye{at}dogwood.botany.uga.edu (E-mail), 706-542-1805 (fax).

Caffeoyl–coenzyme A (CoA) O-methyltransferase (CCoAOMT) has been proposed to be involved in an alternative methylation pathway of lignin biosynthesis. However, no direct evidence has been available to confirm that CCoAOMT is essential for lignin biosynthesis. To understand further the methylation steps in lignin biosynthesis, we used an antisense approach to alter O-methyltransferase (OMT) gene expression and investigated the consequences of this alteration. We generated transgenic tobacco plants with a substantial reduction in CCoAOMT as well as plants with a simultaneous reduction in both CCoAOMT and caffeic acid O-methyltransferase (CAOMT). Lignin analysis showed that the reduction in CCoAOMT alone resulted in a dramatic decrease in lignin content. The reduction in CCoAOMT also led to a dramatic alteration in lignin composition. Both guaiacyl lignin and syringyl lignin were reduced in the transgenic plants. However, guaiacyl lignin was preferentially reduced, which resulted in an increase in the S/G (syringl/guaiacyl) ratio. We have also analyzed lignin content and composition in transgenic plants having a simultaneous reduction in both CCoAOMT and CAOMT. The reduction in both OMTs resulted in a further decrease in total lignin content. This is in sharp contrast to the effect that resulted from the reduction in CAOMT alone, which only decreased the syringl lignin unit without a reduction in overall lignin content. These results unequivocally demonstrate that methylation reactions in lignin biosynthesis are catalyzed by both CCoAOMT and CAOMT.




This article has been cited by other articles:


Home page
 Plant CellHome page
J. Zhou, C. Lee, R. Zhong, and Z.-H. Ye
MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in Arabidopsis
PLANT CELL, January 1, 2009; 21(1): 248 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Zhong, C. Lee, J. Zhou, R. L. McCarthy, and Z.-H. Ye
A Battery of Transcription Factors Involved in the Regulation of Secondary Cell Wall Biosynthesis in Arabidopsis
PLANT CELL, October 1, 2008; 20(10): 2763 - 2782.
[Abstract] [Full Text] [PDF]

Home page
 jashsHome page
Z. Hu, Q. Liu, M. Tan, H. Yi, and X. Deng
Lignin Deposition Converts Juice Sacs to "Brown Thorns" in a Citrus Triploid Hybrid
J. Amer. Soc. Hort. Sci., March 1, 2008; 133(2): 173 - 177.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Dafny-Yelin, S.-M. Chung, E. L. Frankman, and T. Tzfira
pSAT RNA Interference Vectors: A Modular Series for Multiple Gene Down-Regulation in Plants
Plant Physiology, December 1, 2007; 145(4): 1272 - 1281.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Guillaumie, H. San-Clemente, C. Deswarte, Y. Martinez, C. Lapierre, A. Murigneux, Y. Barriere, M. Pichon, and D. Goffner
MAIZEWALL. Database and Developmental Gene Expression Profiling of Cell Wall Biosynthesis and Assembly in Maize
Plant Physiology, January 1, 2007; 143(1): 339 - 363.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N Wuyts, G Lognay, R Swennen, and D De Waele
Nematode infection and reproduction in transgenic and mutant Arabidopsis and tobacco with an altered phenylpropanoid metabolism
J. Exp. Bot., August 1, 2006; 57(11): 2825 - 2835.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Deluc, F. Barrieu, C. Marchive, V. Lauvergeat, A. Decendit, T. Richard, J.-P. Carde, J.-M. Merillon, and S. Hamdi
Characterization of a Grapevine R2R3-MYB Transcription Factor That Regulates the Phenylpropanoid Pathway
Plant Physiology, February 1, 2006; 140(2): 499 - 511.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J.-L. Ferrer, C. Zubieta, R. A. Dixon, and J. P. Noel
Crystal Structures of Alfalfa Caffeoyl Coenzyme A 3-O-Methyltransferase
Plant Physiology, March 1, 2005; 137(3): 1009 - 1017.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
G. SENGUPTA and P. PALIT
Characterization of a Lignified Secondary Phloem Fibre-deficient Mutant of Jute (Corchorus capsularis)
Ann. Bot., February 1, 2004; 93(2): 211 - 220.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. B. Nair, K. L. Bastress, M. O. Ruegger, J. W. Denault, and C. Chapple
The Arabidopsis thaliana REDUCED EPIDERMAL FLUORESCENCE1 Gene Encodes an Aldehyde Dehydrogenase Involved in Ferulic Acid and Sinapic Acid Biosynthesis
PLANT CELL, February 1, 2004; 16(2): 544 - 554.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Oh, S. Park, and K.-H. Han
Transcriptional regulation of secondary growth in Arabidopsis thaliana
J. Exp. Bot., December 1, 2003; 54(393): 2709 - 2722.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Raes, A. Rohde, J. H. Christensen, Y. Van de Peer, and W. Boerjan
Genome-Wide Characterization of the Lignification Toolbox in Arabidopsis
Plant Physiology, November 1, 2003; 133(3): 1051 - 1071.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Kawaoka, E. Matsunaga, S. Endo, S. Kondo, K. Yoshida, A. Shinmyo, and H. Ebinuma
Ectopic Expression of a Horseradish Peroxidase Enhances Growth Rate and Increases Oxidative Stress Resistance in Hybrid Aspen
Plant Physiology, July 1, 2003; 132(3): 1177 - 1185.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. S. AMTHOR
Efficiency of Lignin Biosynthesis: a Quantitative Analysis
Ann. Bot., May 1, 2003; 91(6): 673 - 695.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. B. Nair, Q. Xia, C. J. Kartha, E. Kurylo, R. N. Hirji, R. Datla, and G. Selvaraj
Arabidopsis CYP98A3 Mediating Aromatic 3-Hydroxylation. Developmental Regulation of the Gene, and Expression in Yeast
Plant Physiology, September 1, 2002; 130(1): 210 - 220.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Probing the Mysteries of Lignin Biosynthesis: The Crystal Structure of Caffeic Acid/5-Hydroxyferulic Acid 3/5-O-Methyltransferase Provides New Insights
PLANT CELL, June 1, 2002; 14(6): 1185 - 1189.
[Full Text] [PDF]

Home page
 GeneticsHome page
M. Ruegger and C. Chapple
Mutations That Reduce Sinapoylmalate Accumulation in Arabidopsis thaliana Define Loci With Diverse Roles in Phenylpropanoid Metabolism
Genetics, December 1, 2001; 159(4): 1741 - 1749.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Li, X. F. Cheng, J. Leshkevich, T. Umezawa, S. A. Harding, and V. L. Chiang
The Last Step of Syringyl Monolignol Biosynthesis in Angiosperms Is Regulated by a Novel Gene Encoding Sinapyl Alcohol Dehydrogenase
PLANT CELL, July 1, 2001; 13(7): 1567 - 1586.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Pinçon, M. Chabannes, C. Lapierre, B. Pollet, K. Ruel, J.-P. Joseleau, A. M. Boudet, and M. Legrand
Simultaneous Down-Regulation of Caffeic/5-Hydroxy Ferulic Acid-O-Methyltransferase I and Cinnamoyl-Coenzyme A Reductase in the Progeny from a Cross between Tobacco Lines Homozygous for Each Transgene. Consequences for Plant Development and Lignin Synthesis
Plant Physiology, May 1, 2001; 126(1): 145 - 155.
[Abstract] [Full Text]

Home page
 Plant CellHome page
D. Guo, F. Chen, K. Inoue, J. W. Blount, and R. A. Dixon
Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin
PLANT CELL, January 1, 2001; 13(1): 73 - 88.
[Abstract] [Full Text]

Home page
 Plant CellHome page
J. O. Borevitz, Y. Xia, J. Blount, R. A. Dixon, and C. Lamb
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
PLANT CELL, December 1, 2000; 12(12): 2383 - 2394.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
R. Zhong, W. H. Morrison III, D. S. Himmelsbach, F. L. Poole II, and Z.-H. Ye
Essential Role of Caffeoyl Coenzyme A O-Methyltransferase in Lignin Biosynthesis in Woody Poplar Plants
Plant Physiology, October 1, 2000; 124(2): 563 - 578.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
L. Jouanin, T. Goujon, V. de Nadaï, M.-T. Martin, I. Mila, C. Vallet, B. Pollet, A. Yoshinaga, B. Chabbert, M. Petit-Conil, et al.
Lignification in Transgenic Poplars with Extremely Reduced Caffeic Acid O-Methyltransferase Activity
Plant Physiology, August 1, 2000; 123(4): 1363 - 1374.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
C. Chen, H. Meyermans, B. Burggraeve, R. M. De Rycke, K. Inoue, V. De Vleesschauwer, M. Steenackers, M. C. Van Montagu, G. J. Engler, and W. A. Boerjan
Cell-Specific and Conditional Expression of Caffeoyl-Coenzyme A-3-O-Methyltransferase in Poplar
Plant Physiology, July 1, 2000; 123(3): 853 - 868.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
R. Zhong, A. Ripperger, and Z.-H. Ye
Ectopic Deposition of Lignin in the Pith of Stems of Two Arabidopsis Mutants
Plant Physiology, May 1, 2000; 123(1): 59 - 70.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
L. Li, J. L. Popko, T. Umezawa, and V. L. Chiang
5-Hydroxyconiferyl Aldehyde Modulates Enzymatic Methylation for Syringyl Monolignol Formation, a New View of Monolignol Biosynthesis in Angiosperms
J. Biol. Chem., February 25, 2000; 275(9): 6537 - 6545.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Maury, P. Geoffroy, and M. Legrand
Tobacco O-Methyltransferases Involved in Phenylpropanoid Metabolism. The Different Caffeoyl-Coenzyme A/5-Hydroxyferuloyl-Coenzyme A 3/5-O-Methyltransferase and Caffeic Acid/5-Hydroxyferulic Acid 3/5-O-Methyltransferase Classes Have Distinct Substrate Specificities and Expression Patterns
Plant Physiology, September 1, 1999; 121(1): 215 - 224.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. M. Humphreys, M. R. Hemm, and C. Chapple
New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase
PNAS, August 31, 1999; 96(18): 10045 - 10050.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Meyermans, K. Morreel, C. Lapierre, B. Pollet, A. De Bruyn, R. Busson, P. Herdewijn, B. Devreese, J. Van Beeumen, J. M. Marita, et al.
Modifications in Lignin and Accumulation of Phenolic Glucosides in Poplar Xylem upon Down-regulation of Caffeoyl-Coenzyme A O-Methyltransferase, an Enzyme Involved in Lignin Biosynthesis
J. Biol. Chem., November 17, 2000; 275(47): 36899 - 36909.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Schoch, S. Goepfert, M. Morant, A. Hehn, D. Meyer, P. Ullmann, and D. Werck-Reichhart
CYP98A3 from Arabidopsis thaliana Is a 3'-Hydroxylase of Phenolic Esters, a Missing Link in the Phenylpropanoid Pathway
J. Biol. Chem., September 21, 2001; 276(39): 36566 - 36574.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. C. Abbott, A. Barakate, G. Pincon, M. Legrand, C. Lapierre, I. Mila, W. Schuch, and C. Halpin
Simultaneous Suppression of Multiple Genes by Single Transgenes. Down-Regulation of Three Unrelated Lignin Biosynthetic Genes in Tobacco
Plant Physiology, March 1, 2002; 128(3): 844 - 853.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1998 by the American Society of Plant Biologists