Plant Cell Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (129)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schiebel, W.
Right arrow Articles by Wassenegger, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schiebel, W.
Right arrow Articles by Wassenegger, M.
Agricola
Right arrow Articles by Schiebel, W.
Right arrow Articles by Wassenegger, M.
Plant Cell, Vol. 10, 2087-2102, December 1998, Copyright © 1998, American Society of Plant Physiologists

Isolation of an RNA-Directed RNA Polymerase –Specific cDNA Clone from Tomato

Winfried Schiebela, Thierry Pélissiera, Leonhard Riedelb, Sabine Thalmeira, Rosemarie Schiebela, Dirk Kempea, Friedrich Lottspeichc, Heinz L. Sängera, and Michael Wasseneggera
a Max-Planck-Institut für Biochemie, Abteilung Viroidforschung, D-82152 Martinsried, Germany
b Botanisches Institut, Biologie I der RWTH Aachen, Worringer Weg 1, Germany
c Max-Planck-Institut für Biochemie, Abteilung Proteinchemie, D-82152 Martinsried, Germany

Correspondence to: Michael Wassenegger, wasseneg{at}biochem.mpg.de (E-mail), 49-89-8578-2937 (fax).

A 3600-bp RNA-directed RNA polymerase (RdRP)–specific cDNA comprising an open reading frame (ORF) of 1114 amino acids was isolated from tomato. The putative protein encoded by this ORF does not share homology with any characterized proteins. Antibodies that were raised against synthetic peptides whose sequences have been deduced from the ORF were shown to specifically detect the 127-kD tomato RdRP protein. The immunoresponse to the antibodies correlated with the enzymatic activity profile of the RdRP after chromatography on Q-, poly(A)–, and poly(U)–Sepharose, hydroxyapatite, and Sephadex G-200 columns. DNA gel blot analysis revealed a single copy of the RdRP gene in tomato. RdRP homologs from petunia, Arabidopsis, tobacco, and wheat were identified by using polymerase chain reaction. A sequence comparison indicated that sequences homologous to RdRP are also present in the yeast Schizosaccharomyces pombe and in the nematode Caenorhabditis elegans. The previously described induction of RdRP activity upon viroid infection is shown to be correlated with an increased steady state level of the corresponding mRNA. The possible involvement of this heretofore functionally elusive plant RNA polymerase in homology-dependent gene silencing is discussed.




This article has been cited by other articles:


Home page
 J. Virol.Home page
F. Di Serio, A.-E. Martinez de Alba, B. Navarro, A. Gisel, and R. Flores
RNA-Dependent RNA Polymerase 6 Delays Accumulation and Precludes Meristem Invasion of a Viroid That Replicates in the Nucleus
J. Virol., March 1, 2010; 84(5): 2477 - 2489.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. P. Pandey, E. Gaquerel, K. Gase, and I. T. Baldwin
RNA-Directed RNA Polymerase3 from Nicotiana attenuata Is Required for Competitive Growth in Natural Environments
Plant Physiology, July 1, 2008; 147(3): 1212 - 1224.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Curaba and X. Chen
Biochemical Activities of Arabidopsis RNA-dependent RNA Polymerase 6
J. Biol. Chem., February 8, 2008; 283(6): 3059 - 3066.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Li, L. Xu, H. Wang, Z. Yuan, X. Cao, Z. Yang, D. Zhang, Y. Xu, and H. Huang
The Putative RNA-Dependent RNA Polymerase RDR6 Acts Synergistically with ASYMMETRIC LEAVES1 and 2 to Repress BREVIPEDICELLUS and MicroRNA165/166 in Arabidopsis Leaf Development
PLANT CELL, August 1, 2005; 17(8): 2157 - 2171.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Schwach, F. E. Vaistij, L. Jones, and D. C. Baulcombe
An RNA-Dependent RNA Polymerase Prevents Meristem Invasion by Potato Virus X and Is Required for the Activity But Not the Production of a Systemic Silencing Signal
Plant Physiology, August 1, 2005; 138(4): 1842 - 1852.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. M. C. Lai
RNA Replication without RNA-Dependent RNA Polymerase: Surprises from Hepatitis Delta Virus
J. Virol., July 1, 2005; 79(13): 7951 - 7958.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Tomari and P. D. Zamore
Perspective: machines for RNAi
Genes & Dev., March 1, 2005; 19(5): 517 - 529.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
O. Mathieu and J. Bender
RNA-directed DNA methylation
J. Cell Sci., October 1, 2004; 117(21): 4881 - 4888.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Gallei, A. Pankraz, H.-J. Thiel, and P. Becher
RNA Recombination In Vivo in the Absence of Viral Replication
J. Virol., June 15, 2004; 78(12): 6271 - 6281.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S.-J. Yang, S. A. Carter, A. B. Cole, N.-H. Cheng, and R. S. Nelson
A natural variant of a host RNA-dependent RNA polymerase is associated with increased susceptibility to viruses by Nicotiana benthamiana
PNAS, April 20, 2004; 101(16): 6297 - 6302.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
N. Agrawal, P. V. N. Dasaradhi, A. Mohmmed, P. Malhotra, R. K. Bhatnagar, and S. K. Mukherjee
RNA Interference: Biology, Mechanism, and Applications
Microbiol. Mol. Biol. Rev., December 1, 2003; 67(4): 657 - 685.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. P. GMYL, S. A. KORSHENKO, E. V. BELOUSOV, E. V. KHITRINA, and V. I. AGOL
Nonreplicative homologous RNA recombination: Promiscuous joining of RNA pieces?
RNA, October 1, 2003; 9(10): 1221 - 1231.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
M. N. ALDER, S. DAMES, J. GAUDET, and S. E. MANGO
Gene silencing in Caenorhabditis elegans by transitive RNA interference
RNA, January 1, 2003; 9(1): 25 - 32.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. Qu, T. Ren, and T. J. Morris
The Coat Protein of Turnip Crinkle Virus Suppresses Posttranscriptional Gene Silencing at an Early Initiation Step
J. Virol., January 1, 2003; 77(1): 511 - 522.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
G. Tang, B. J. Reinhart, D. P. Bartel, and P. D. Zamore
A biochemical framework for RNA silencing in plants
Genes & Dev., January 1, 2003; 17(1): 49 - 63.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. A. Jorgensen
RNA traffics information systemically in plants
PNAS, September 3, 2002; 99(18): 11561 - 11563.
[Full Text] [PDF]

Home page
 ScienceHome page
R. H. A. Plasterk
RNA Silencing: The Genome's Immune System
Science, May 17, 2002; 296(5571): 1263 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. B. Macnaughton, S. T. Shi, L. E. Modahl, and M. M. C. Lai
Rolling Circle Replication of Hepatitis Delta Virus RNA Is Carried Out by Two Different Cellular RNA Polymerases
J. Virol., April 15, 2002; 76(8): 3920 - 3927.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Anantharaman, E. V. Koonin, and L. Aravind
Comparative genomics and evolution of proteins involved in RNA metabolism
Nucleic Acids Res., April 1, 2002; 30(7): 1427 - 1464.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
I. Mitsuhara, N. Shirasawa-Seo, T. Iwai, S. Nakamura, R. Honkura, and Y. Ohashi
Release From Post-transcriptional Gene Silencing by Cell Proliferation in Transgenic Tobacco Plants: Possible Mechanism for Noninheritance of the Silencing
Genetics, January 1, 2002; 160(1): 343 - 352.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Galvani and L. Sperling
Transgene-mediated post-transcriptional gene silencing is inhibited by 3' non-coding sequences in Paramecium
Nucleic Acids Res., November 1, 2001; 29(21): 4387 - 4394.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Moraleda and J. Taylor
Host RNA Polymerase Requirements for Transcription of the Human Hepatitis Delta Virus Genome
J. Virol., November 1, 2001; 75(21): 10161 - 10169.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Horiuchi, T. Udagawa, R. Koga, H. Moriyama, and T. Fukuhara
RNA-Dependent RNA Polymerase Activity Associated with Endogenous Double-Stranded RNA in Rice
Plant Cell Physiol., February 1, 2001; 42(2): 197 - 203.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. E. Domeier, D. P. Morse, S. W. Knight, M. Portereiko, B. L. Bass, and S. E. Mango
A Link Between RNA Interference and Nonsense-Mediated Decay in Caenorhabditis elegans
Science, September 15, 2000; 289(5486): 1928 - 1930.
[Abstract] [Full Text]

Home page
 J. Gen. Virol.Home page
F.-J. Jan, S.-Z. Pang, D. M. Tricoli, and D. Gonsalves
Evidence that resistance in squash mosaic comovirus coat protein-transgenic plants is affected by plant developmental stage and enhanced by combination of transgenes from different lines
J. Gen. Virol., September 1, 2000; 81(9): 2299 - 2306.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
A. F. Dernburg, J. Zalevsky, M. P. Colaiácovo, and A. M. Villeneuve
Transgene-mediated cosuppression in the C. elegans germ line
Genes & Dev., July 1, 2000; 14(13): 1578 - 1583.
[Abstract] [Full Text]

Home page
 GeneticsHome page
S. Jensen, M.-P. Gassama, and T. Heidmann
Cosuppression of I Transposon Activity in Drosophila by I-Containing Sense and Antisense Transgenes
Genetics, December 1, 1999; 153(4): 1767 - 1774.
[Abstract] [Full Text]

Home page
 ScienceHome page
A. P. Wolffe and M. A. Matzke
Epigenetics: Regulation Through Repression
Science, October 15, 1999; 286(5439): 481 - 486.
[Abstract] [Full Text]

Home page
 Plant CellHome page
H. B. Smith
Interfering with Viral Infection: Plants Do It Too
PLANT CELL, July 1, 1999; 11(7): 1191 - 1194.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C.-F. Chuang and E. M. Meyerowitz
Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana
PNAS, April 25, 2000; 97(9): 4985 - 4990.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Xie, B. Fan, C. Chen, and Z. Chen
An important role of an inducible RNA-dependent RNA polymerase in plant antiviral defense
PNAS, May 22, 2001; 98(11): 6516 - 6521.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Aravind, H. Watanabe, D. J. Lipman, and E. V. Koonin
Lineage-specific loss and divergence of functionally linked genes in eukaryotes
PNAS, October 10, 2000; 97(21): 11319 - 11324.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1998 by the American Society of Plant Biologists