Plant Cell Huazhong Agricultural University
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (63)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kranz, E.
Right arrow Articles by Lörz, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kranz, E.
Right arrow Articles by Lörz, H.
Agricola
Right arrow Articles by Kranz, E.
Right arrow Articles by Lörz, H.
Plant Cell, Vol. 10, 511-524, April 1998, Copyright © 1998, American Society of Plant Physiologists

Endosperm Development after Fusion of Isolated, Single Maize Sperm and Central Cells in Vitro

Erhard Kranza, Petra von Wiegena, Hartmut Quadera, and Horst Lörza
a Institut für Allgemeine Botanik, Angewandte Molekularbiologie der Pflanzen II, Universität Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany

Correspondence to: Erhard Kranz, ekranz{at}botanik.uni-hamburg.de (E-mail), 49-40-822-822-29 (fax).

We demonstrate here the possibility of endosperm development in vitro after the fusion of pairs of an isolated sperm and an isolated central cell of maize. The occurrence of karyogamy and the time course of the fusion of sperm and central cell nuclei are presented. The fusion of the sperm nucleus occurred either with one of the two polar nuclei or with the secondary nucleus and was completed within 2 hr after in vitro cell fusion. The in vitro study of early events after cell and nuclear fusion indicates that the resulting primary endosperm cell develops into a characteristic tissue capable of self-organization apart from the mother tissue. The technology presented here opens the way for new cellular and molecular studies, especially of early events after sperm and central cell fusion. These studies should lead to a better understanding of the processes of double fertilization and endosperm development.




This article has been cited by other articles:


Home page
 Mol PlantHome page
R. J. Scott, S. J. Armstrong, J. Doughty, and M. Spielman
Double Fertilization in Arabidopsis thaliana Involves a Polyspermy Block on the Egg but Not the Central Cell
Mol Plant, July 1, 2008; 1(4): 611 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Gruis, H. Guo, D. Selinger, Q. Tian, and O.-A. Olsen
Surface Position, Not Signaling from Surrounding Maternal Tissues, Specifies Aleurone Epidermal Cell Fate in Maize
Plant Physiology, July 1, 2006; 141(3): 898 - 909.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Dresselhaus, K.-o. Srilunchang, D. Leljak-Levanic, D. N. Schreiber, and P. Garg
The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development
Plant Physiology, February 1, 2006; 140(2): 512 - 527.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
G. C. Pagnussat, H.-J. Yu, Q. A. Ngo, S. Rajani, S. Mayalagu, C. S. Johnson, A. Capron, L.-F. Xie, D. Ye, and V. Sundaresan
Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis
Development, February 1, 2005; 132(3): 603 - 614.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Okamoto, K. Higuchi, T. Shinkawa, T. Isobe, H. Lorz, T. Koshiba, and E. Kranz
Identification of Major Proteins in Maize Egg Cells
Plant Cell Physiol., October 15, 2004; 45(10): 1406 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. F. Gutierrez-Marcos, L. M. Costa, C. Biderre-Petit, B. Khbaya, D. M. O'Sullivan, M. Wormald, P. Perez, and H. G. Dickinson
maternally expressed gene1 Is a Novel Maize Endosperm Transfer Cell-Specific Gene with a Maternal Parent-of-Origin Pattern of Expression
PLANT CELL, May 1, 2004; 16(5): 1288 - 1301.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P. E. Grini, G. Jurgens, and M. Hulskamp
Embryo and Endosperm Development Is Disrupted in the Female Gametophytic capulet Mutants of Arabidopsis
Genetics, December 1, 2002; 162(4): 1911 - 1925.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J.-E. Faure and C. Dumas
Fertilization in Flowering plants. New Approaches for an Old Story
Plant Physiology, January 1, 2001; 125(1): 102 - 104.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. F. Antoine, J.-E. Faure, S. Cordeiro, C. Dumas, M. Rougier, and J. A. Feijó
A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants
PNAS, September 5, 2000; (2000) 180243697.
[Abstract] [Full Text]

Home page
 Plant CellHome page
V. E. Franklin-Tong
Signaling and the Modulation of Pollen Tube Growth
PLANT CELL, April 1, 1999; 11(4): 727 - 738.
[Full Text]

Home page
 Plant CellHome page
T. Higashiyama, H. Kuroiwa, S. Kawano, and T. Kuroiwa
Guidance in Vitro of the Pollen Tube to the Naked Embryo Sac of Torenia fournieri
PLANT CELL, December 1, 1998; 10(12): 2019 - 2032.
[Abstract] [Full Text]

Home page
 Plant CellHome page
O.-A. Olsen
Endosperm Developments
PLANT CELL, April 1, 1998; 10(4): 485 - 488.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. F. Antoine, J.-E. Faure, S. Cordeiro, C. Dumas, M. Rougier, and J. A. Feijo
A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants
PNAS, September 12, 2000; 97(19): 10643 - 10648.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Gomez, J. Royo, Y. Guo, R. Thompson, and G. Hueros
Establishment of Cereal Endosperm Expression Domains: Identification and Properties of a Maize Transfer Cell-Specific Transcription Factor, ZmMRP-1
PLANT CELL, March 1, 2002; 14(3): 599 - 610.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1998 by the American Society of Plant Biologists