Plant Cell Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Plant Cell
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (130)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grotewold, E.
Right arrow Articles by Bowen, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grotewold, E.
Right arrow Articles by Bowen, B.
Agricola
Right arrow Articles by Grotewold, E.
Right arrow Articles by Bowen, B.
Plant Cell, Vol. 10, 721-740, May 1998, Copyright © 1998, American Society of Plant Physiologists

Engineering Secondar y Metabolism in Maize Cells by Ectopic Expression of Transcription Factors

Erich Grotewolda, Mark Chamberlinb, Maurice Snookc, Bupe Siamed, Larry Butlerd, Jan Swensonb, Sheila Maddockb, Grace St. Clairb, and Ben Bowenb
a Cold Spring Harbor Laboratory, 1 Bungtown Road, P.O. Box 100, Cold Spring Harbor, New York 11724
b Pioneer Hi-Bred International, Inc., 7300 N.W. 62nd Avenue, P.O. Box 1004, Johnston, Iowa 50131
c U.S. Department of Agriculture–Agriculture Research Service, Russell Research Center, P.O. Box 5677, Athens, Georgia 30604
d Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907

Correspondence to: Ben Bowen, bowenb{at}phibred.com (E-mail), 515-270-3367 (fax).

Manipulation of plant natural product biosynthesis through genetic engineering is an attractive but technically challenging goal. Here, we demonstrate that different secondary metabolites can be produced in cultured maize cells by ectopic expression of the appropriate regulatory genes. Cell lines engineered to express the maize transcriptional activators C1 and R accumulate two cyanidin derivatives, which are similar to the predominant anthocyanin found in differentiated plant tissues. In contrast, cell lines that express P accumulate various 3-deoxy flavonoids. Unexpectedly, P-expressing cells in culture also accumulate phenylpropanoids and green fluorescent compounds that are targeted to different subcellular compartments. Two endogenous biosynthetic genes (c2 and a1, encoding chalcone synthase and flavanone/dihydroflavonol reductase, respectively) are independently activated by ectopic expression of either P or C1/R, and there is a dose–response relationship between the transcript level of P and the degree to which c2 or a1 is expressed. Our results support a simple model showing how the gene encoding P may act as a quantitative trait locus controlling insecticidal C-glycosyl flavone level in maize silks, and they suggest how p1 might confer a selective advantage against insect predation in maize.


Related articles in Plant Cell:

Factories of the Future? Metabolic Engineering in Plant Cells
Crispin B. Taylor
Plant Cell 1998 10: 641-644. [Full Text]  



This article has been cited by other articles:


Home page
 Mol PlantHome page
L. Pourcel, N. G. Irani, Y. Lu, K. Riedl, S. Schwartz, and E. Grotewold
The Formation of Anthocyanic Vacuolar Inclusions in Arabidopsis thaliana and Implications for the Sequestration of Anthocyanin Pigments
Mol Plant, January 1, 2010; 3(1): 78 - 90.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Du, H. Chu, M. Wang, I. K. Chu, and C. Lo
Identification of flavone phytoalexins and a pathogen-inducible flavone synthase II gene (SbFNSII) in sorghum
J. Exp. Bot., December 10, 2009; (2009) erp364v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Gomez, N. Terrier, L. Torregrosa, S. Vialet, A. Fournier-Level, C. Verries, J.-M. Souquet, J.-P. Mazauric, M. Klein, V. Cheynier, et al.
Grapevine MATE-Type Proteins Act as Vacuolar H+-Dependent Acylated Anthocyanin Transporters
Plant Physiology, May 1, 2009; 150(1): 402 - 415.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. S. Sekhon and S. Chopra
Progressive Loss of DNA Methylation Releases Epigenetic Gene Silencing From a Tandemly Repeated Maize Myb Gene
Genetics, January 1, 2009; 181(1): 81 - 91.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. A. E. Laitinen, M. Ainasoja, S. K. Broholm, T. H. Teeri, and P. Elomaa
Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida
J. Exp. Bot., October 1, 2008; 59(13): 3691 - 3703.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Conn, C. Curtin, A. Bezier, C. Franco, and W. Zhang
Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins
J. Exp. Bot., October 1, 2008; 59(13): 3621 - 3634.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. C. Palmieri, S. Sell, X. Huang, M. Scherf, T. Werner, J. Durner, and C. Lindermayr
Nitric oxide-responsive genes and promoters in Arabidopsis thaliana: a bioinformatics approach
J. Exp. Bot., February 13, 2008; (2008) erm345v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Poustka, N. G. Irani, A. Feller, Y. Lu, L. Pourcel, K. Frame, and E. Grotewold
A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions
Plant Physiology, December 1, 2007; 145(4): 1323 - 1335.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. M. Hernandez, A. Feller, K. Morohashi, K. Frame, and E. Grotewold
The basic helix loop helix domain of maize R links transcriptional regulation and histone modifications by recruitment of an EMSY-related factor
PNAS, October 23, 2007; 104(43): 17222 - 17227.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Zhang, S. Subramanian, Y. Zhang, and O. Yu
Flavone Synthases from Medicago truncatula Are Flavanone-2-Hydroxylases and Are Important for Nodulation
Plant Physiology, June 1, 2007; 144(2): 741 - 751.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Feller, J. M. Hernandez, and E. Grotewold
An ACT-like Domain Participates in the Dimerization of Several Plant Basic-helix-loop-helix Transcription Factors
J. Biol. Chem., September 29, 2006; 281(39): 28964 - 28974.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. Sreevidya, C Srinivasa Rao, S. Sullia, J. K. Ladha, and P. M. Reddy
Metabolic engineering of rice with soybean isoflavone synthase for promoting nodulation gene expression in rhizobia
J. Exp. Bot., June 1, 2006; 57(9): 1957 - 1969.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. E. Saslowsky, U. Warek, and B. S. J. Winkel
Nuclear Localization of Flavonoid Enzymes in Arabidopsis
J. Biol. Chem., June 24, 2005; 280(25): 23735 - 23740.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Z. Swigonova, J. L. Bennetzen, and J. Messing
Structure and Evolution of the r/b Chromosomal Regions in Rice, Maize and Sorghum
Genetics, February 1, 2005; 169(2): 891 - 906.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Hernandez, G. F. Heine, N. G. Irani, A. Feller, M.-G. Kim, T. Matulnik, V. L. Chandler, and E. Grotewold
Different Mechanisms Participate in the R-dependent Activity of the R2R3 MYB Transcription Factor C1
J. Biol. Chem., November 12, 2004; 279(46): 48205 - 48213.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y.-S. Hwang, P. Ciceri, R. L. Parsons, S. P. Moose, R. J. Schmidt, and N. Huang
The Maize O2 and PBF Proteins Act Additively to Promote Transcription from Storage Protein Gene Promoters in Rice Endosperm Cells
Plant Cell Physiol., October 15, 2004; 45(10): 1509 - 1518.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. F. Heine, J. M. Hernandez, and E. Grotewold
Two Cysteines in Plant R2R3 MYB Domains Participate in REDOX-dependent DNA Binding
J. Biol. Chem., September 3, 2004; 279(36): 37878 - 37885.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
M. D. McMullen, H. Kross, M. E. Snook, M. Cortes-Cruz, K. E. Houchins, T. A. Musket, and E. H. Coe Jr
Salmon Silk Genes Contribute to the Elucidation of the Flavone Pathway in Maize (Zea mays L.)
J. Hered., May 1, 2004; 95(3): 225 - 233.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Zuther, S. Huang, J. Jelenska, H. Eilenberg, E. M. Arnold, X. Su, A. Sirikhachornkit, J. Podkowinski, A. Zilberstein, R. Haselkorn, et al.
Complex nested promoters control tissue-specific expression of acetyl-CoA carboxylase genes in wheat
PNAS, February 3, 2004; 101(5): 1403 - 1408.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Himi and K. Noda
Isolation and location of three homoeologous dihydroflavonol-4-reductase (DFR) genes of wheat and their tissue-dependent expression
J. Exp. Bot., February 1, 2004; 55(396): 365 - 375.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. C. Carey, J. T. Strahle, D. A. Selinger, and V. L. Chandler
Mutations in the pale aleurone color1 Regulatory Gene of the Zea mays Anthocyanin Pathway Have Distinct Phenotypes Relative to the Functionally Similar TRANSPARENT TESTA GLABRA1 Gene in Arabidopsis thaliana
PLANT CELL, February 1, 2004; 16(2): 450 - 464.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. S. Walker, H. P. Bais, E. Grotewold, and J. M. Vivanco
Root Exudation and Rhizosphere Biology
Plant Physiology, May 1, 2003; 132(1): 44 - 51.
[Full Text] [PDF]

Home page
 J Exp BotHome page
M. P. Robbins, F. Paolocci, J.-W. Hughes, V. Turchetti, G. Allison, S. Arcioni, P. Morris, and F. Damiani
Sn, a maize bHLH gene, modulates anthocyanin and condensed tannin pathways in Lotus corniculatus
J. Exp. Bot., January 2, 2003; 54(381): 239 - 248.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Bovy, R. de Vos, M. Kemper, E. Schijlen, M. Almenar Pertejo, S. Muir, G. Collins, S. Robinson, M. Verhoeyen, S. Hughes, et al.
High-Flavonol Tomatoes Resulting from the Heterologous Expression of the Maize Transcription Factor Genes LC and C1
PLANT CELL, October 1, 2002; 14(10): 2509 - 2526.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
B. S. Bushman, M. E. Snook, J. P. Gerke, S. J. Szalma, M. A. Berhow, K. E. Houchins, and M. D. McMullen
Two Loci Exert Major Effects on Chlorogenic Acid Synthesis in Maize Silks
Crop Sci., September 1, 2002; 42(5): 1669 - 1678.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
S. J. Szalma, M. E. Snook, B. S. Bushman, K. E. Houchins, and M. D. McMullen
Duplicate Loci as QTL: The Role of Chalcone Synthase Loci in Flavone and Phenylpropanoid Biosynthesis in Maize
Crop Sci., September 1, 2002; 42(5): 1679 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Brouwer, W. Bruce, S. Maddock, Z. Avramova, and B. Bowen
Suppression of Transgene Silencing by Matrix Attachment Regions in Maize: A Dual Role for the Maize 5' ADH1 Matrix Attachment Region
PLANT CELL, September 1, 2002; 14(9): 2251 - 2264.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Dong, E. L. Braun, and E. Grotewold
Functional Conservation of Plant Secondary Metabolic Enzymes Revealed by Complementation of Arabidopsis Flavonoid Mutants with Maize Genes
Plant Physiology, September 1, 2001; 127(1): 46 - 57.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
I. Debeaujon, A. J. M. Peeters, K. M. Léon-Kloosterziel, and M. Koornneef
The TRANSPARENT TESTA12 Gene of Arabidopsis Encodes a Multidrug Secondary Transporter-like Protein Required for Flavonoid Sequestration in Vacuoles of the Seed Coat Endothelium
PLANT CELL, April 1, 2001; 13(4): 853 - 872.
[Abstract] [Full Text]

Home page
 Plant CellHome page
S. Henkes, U. Sonnewald, R. Badur, R. Flachmann, and M. Stitt
A Small Decrease of Plastid Transketolase Activity in Antisense Tobacco Transformants Has Dramatic Effects on Photosynthesis and Phenylpropanoid Metabolism
PLANT CELL, March 1, 2001; 13(3): 535 - 551.
[Abstract] [Full Text]

Home page
 Plant CellHome page
P. Zhang, S. Chopra, and T. Peterson
A Segmental Gene Duplication Generated Differentially Expressed myb-Homologous Genes in Maize
PLANT CELL, December 1, 2000; 12(12): 2311 - 2322.
[Abstract] [Full Text]

Home page
 Plant CellHome page
J. O. Borevitz, Y. Xia, J. Blount, R. A. Dixon, and C. Lamb
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
PLANT CELL, December 1, 2000; 12(12): 2383 - 2394.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Grotewold, M. B. Sainz, L. Tagliani, J. M. Hernandez, B. Bowen, and V. L. Chandler
Identification of the residues in the Myb domain of maize C1 that specify the interaction with the bHLH cofactor R
PNAS, November 22, 2000; (2000) 250379897.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
O. Yu, W. Jung, J. Shi, R. A. Croes, G. M. Fader, B. McGonigle, and J. T. Odell
Production of the Isoflavones Genistein and Daidzein in Non-Legume Dicot and Monocot Tissues
Plant Physiology, October 1, 2000; 124(2): 781 - 794.
[Abstract] [Full Text]

Home page
 GeneticsHome page
K. Petroni, E. Cominelli, G. Consonni, G. Gusmaroli, G. Gavazzi, and C. Tonelli
The Developmental Expression of the Maize Regulatory Gene Hopi Determines Germination-Dependent Anthocyanin Accumulation
Genetics, May 1, 2000; 155(1): 323 - 336.
[Abstract] [Full Text]

Home page
 Plant CellHome page
W. Bruce, O. Folkerts, C. Garnaat, O. Crasta, B. Roth, and B. Bowen
Expression Profiling of the Maize Flavonoid Pathway Genes Controlled by Estradiol-Inducible Transcription Factors CRC and P
PLANT CELL, January 1, 2000; 12(1): 65 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. A. Selinger and V. L. Chandler
Major recent and independent changes in levels and patterns of expression have occurred at the b gene, a regulatory locus in maize
PNAS, December 21, 1999; 96(26): 15007 - 15012.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P. D. Rabinowicz, E. L. Braun, A. D. Wolfe, B. Bowen, and E. Grotewold
Maize R2R3 Myb Genes: Sequence Analysis Reveals Amplification in the Higher Plants
Genetics, September 1, 1999; 153(1): 427 - 444.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W.-H. Cheng, E. W. Taliercio, and P. S. Chourey
Sugars modulate an unusual mode of control of the cell-wall invertase gene (Incw1) through its 3' untranslated region in a cell suspension culture of maize
PNAS, August 31, 1999; 96(18): 10512 - 10517.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Winicov and D. R. Bastola
Transgenic Overexpression of the Transcription Factor Alfin1 Enhances Expression of the Endogenous MsPRP2 Gene in Alfalfa and Improves Salinity Tolerance of the Plants
Plant Physiology, June 1, 1999; 120(2): 473 - 480.
[Abstract] [Full Text]

Home page
 Plant CellHome page
C. B. Taylor
Factories of the Future? Metabolic Engineering in Plant Cells
PLANT CELL, May 1, 1998; 10(5): 641 - 644.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Grotewold, M. B. Sainz, L. Tagliani, J. M. Hernandez, B. Bowen, and V. L. Chandler
Identification of the residues in the Myb domain of maize C1 that specify the interaction with the bHLH cofactor R
PNAS, December 5, 2000; 97(25): 13579 - 13584.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1998 by the American Society of Plant Biologists