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Plant Cell, Vol. 10, 1233-1250, August 1998, Copyright © 1998, American Society of Plant Physiologists

Lotus corniculatus Nodulation Specificity Is Changed by the Presence of a Soybean Lectin Gene

Pieternel van Rhijna, Robert B. Goldberga,b, and Ann M. Hirscha,b
a Department of Molecular, Cell, and Developmental Biology, 405 Hilgard Avenue, University of California, Los Angeles, California 90095-1606
b Molecular Biology Institute, 405 Hilgard Avenue, University of California, Los Angeles, California 90095-1606

Correspondence to: Ann M. Hirsch, ahirsch{at}ucla.edu (E-mail), 310-206-8673 (fax).

Plant lectins have been implicated as playing an important role in mediating recognition and specificity in the Rhizobium–legume nitrogen-fixing symbiosis. To test this hypothesis, we introduced the soybean lectin gene Le1 either behind its own promoter or behind the cauliflower mosaic virus 35S promoter into Lotus corniculatus, which is nodulated by R. loti. We found that nodulelike outgrowths developed on transgenic L. corniculatus plant roots in response to Bradyrhizobium japonicum, which nodulates soybean and not Lotus spp. Soybean lectin was properly targeted to L. corniculatus root hairs, and although infection threads formed, they aborted in epidermal or hypodermal cells. Mutation of the lectin sugar binding site abolished infection thread formation and nodulation. Incubation of bradyrhizobia in the nodulation (nod) gene–inducing flavonoid genistein increased the number of nodulelike outgrowths on transgenic L. corniculatus roots. Studies of bacterial mutants, however, suggest that a component of the exopolysaccharide surface of B. japonicum, rather than Nod factor, is required for extension of host range to the transgenic L. corniculatus plants.




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