Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (68)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carroll, A. D.
Right arrow Articles by Brownlee, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carroll, A. D.
Right arrow Articles by Brownlee, C.
Agricola
Right arrow Articles by Carroll, A. D.
Right arrow Articles by Brownlee, C.
Plant Cell, Vol. 10, 1267-1276, August 1998, Copyright © 1998, American Society of Plant Physiologists

Ca2+, Annexins, and GTP Modulate Exocytosis from Maize Root Cap Protoplasts

Andrew D. Carrolla, Christelle Moyena, Pim Van Kesterenb, Fiona Tookeb, Nicholas H. Batteyb, and Colin Brownleea
a Marine Biological Association, Citadel Hill, Plymouth PL1 2PB, United Kingdom
b Plant Science Laboratories, University of Reading, Whiteknights, Reading RG6 6AS, United Kingdom

Correspondence to: Nicholas H. Battey, n.h.battey{at}reading.ac.uk (E-mail), 44-118-9750630 (fax).

Protoplasts isolated from root cap cells of maize were shown to secrete fucose-rich polysaccharides and were used in a patch–clamp study to monitor changes in whole-cell capacitance. Ca2+ was required for exocytosis, which was measured as an increase in cell capacitance during intracellular dialysis with Ca2+ buffers via the patch pipette. Exocytosis was stimulated significantly by small increases above normal resting [Ca2+]. In the absence of Ca2+, protoplasts decreased in size. In situ hybridization showed significant expression of the maize annexin p35 in root cap cells, differ-entiating vascular tissue, and elongating cells. Dialysis of protoplasts with maize annexins stimulated exocytosis at physiological [Ca2+], and this could be blocked by dialysis with antibodies specific to maize annexins. Dialysis with milli-molar concentrations of GTP strongly inhibited exocytosis, causing protoplasts to decrease in size. GTP{gamma}S and GDPßS both caused only a slight inhibition of exocytosis at physiological Ca2+. Protoplasts were shown to internalize plasma membrane actively. The results are discussed in relation to the regulation of exocytosis in what is usually considered to be a constitutively secreting system; they provide direct evidence for a role of annexins in exocytosis in plant cells.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
G. B. Monshausen, M. A. Messerli, and S. Gilroy
Imaging of the Yellow Cameleon 3.6 Indicator Reveals That Elevations in Cytosolic Ca2+ Follow Oscillating Increases in Growth in Root Hairs of Arabidopsis
Plant Physiology, August 1, 2008; 147(4): 1690 - 1698.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N.-J. Hu, A. M. Yusof, A. Winter, A. Osman, A. K. Reeve, and A. Hofmann
The Crystal Structure of Calcium-bound Annexin Gh1 from Gossypium hirsutum and Its Implications for Membrane Binding Mechanisms of Plant Annexins
J. Biol. Chem., June 27, 2008; 283(26): 18314 - 18322.
[Abstract] [Full Text] [PDF]

Home page
 CSH ProtocolsHome page
W. Zhang, S. E. Nilson, and S. M. Assmann
Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts
CSH Protocols, June 1, 2008; 2008(7): pdb.prot5014 - pdb.prot5014.
[Abstract] [Full Text]

Home page
 Mol PlantHome page
S. Sokolovski, A. Hills, R. A. Gay, and M. R. Blatt
Functional Interaction of the SNARE Protein NtSyp121 in Ca2+ Channel Gating, Ca2+ Transients and ABA Signalling of Stomatal Guard Cells
Mol Plant, March 1, 2008; 1(2): 347 - 358.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. C. Mortimer, A. Laohavisit, N. Macpherson, A. Webb, C. Brownlee, N. H. Battey, and J. M. Davies
Annexins: multifunctional components of growth and adaptation
J. Exp. Bot., February 10, 2008; (2008) erm344v1.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
T. J. Bushart and S. J. Roux
Conserved Features of Germination and Polarized Cell Growth: A Few Insights from a Pollen-Fern Spore Comparison
Ann. Bot., January 1, 2007; 99(1): 9 - 17.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Campanoni and M. R. Blatt
Membrane trafficking and polar growth in root hairs and pollen tubes
J. Exp. Bot., January 1, 2007; 58(1): 65 - 74.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S.-Y. Kim, M. Sivaguru, and G. Stacey
Extracellular ATP in Plants. Visualization, Localization, and Analysis of Physiological Significance in Growth and Signaling
Plant Physiology, November 1, 2006; 142(3): 984 - 992.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-U. Sutter, P. Campanoni, M. Tyrrell, and M. R. Blatt
Selective Mobility and Sensitivity to SNAREs Is Exhibited by the Arabidopsis KAT1 K+ Channel at the Plasma Membrane
PLANT CELL, April 1, 2006; 18(4): 935 - 954.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Okamoto, K. Higuchi, T. Shinkawa, T. Isobe, H. Lorz, T. Koshiba, and E. Kranz
Identification of Major Proteins in Maize Egg Cells
Plant Cell Physiol., October 15, 2004; 45(10): 1406 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. J. Rudd and V. E. Franklin-Tong
Signals and targets of the self-incompatibility response in pollen of Papaver rhoeas
J. Exp. Bot., January 1, 2003; 54(380): 141 - 148.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Takeda and K. Kasamo
In Vitro Fusion of Plant Golgi Membranes Can Be Influenced by Divalent Cations
J. Biol. Chem., November 27, 2002; 277(49): 47756 - 47764.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Kutsuna and S. Hasezawa
Dynamic Organization of Vacuolar and Microtubule Structures during Cell Cycle Progression in Synchronized Tobacco BY-2 Cells
Plant Cell Physiol., September 15, 2002; 43(9): 965 - 973.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Geelen, B. Leyman, H. Batoko, G.-P. Di Sansebastiano, I. Moore, and M. R. Blatt
The Abscisic Acid-Related SNARE Homolog NtSyr1 Contributes to Secretion and Growth: Evidence from Competition with Its Cytosolic Domain
PLANT CELL, February 1, 2002; 14(2): 387 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Emans, S. Zimmermann, and R. Fischer
Uptake of a Fluorescent Marker in Plant Cells Is Sensitive to Brefeldin A and Wortmannin
PLANT CELL, January 1, 2002; 14(1): 71 - 86.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. B. Clark, A. Sessions, D. J. Eastburn, and S. J. Roux
Differential Expression of Members of the Annexin Multigene Family in Arabidopsis
Plant Physiology, July 1, 2001; 126(3): 1072 - 1084.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Yuasa and M. Maeshima
Purification, Properties, and Molecular Cloning of a Novel Ca2+-Binding Protein in Radish Vacuoles
Plant Physiology, November 1, 2000; 124(3): 1069 - 1078.
[Abstract] [Full Text]

Home page
 Plant CellHome page
J.-U. Sutter, U. Homann, and G. Thiel
Ca2+-Stimulated Exocytosis in Maize Coleoptile Cells
PLANT CELL, July 1, 2000; 12(7): 1127 - 1136.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
S Lecat, P Verkade, C Thiele, K Fiedler, K Simons, and F Lafont
Different properties of two isoforms of annexin XIII in MDCK cells
J. Cell Sci., January 7, 2000; 113(14): 2607 - 2618.
[Abstract] [PDF]

Home page
 Plant Physiol.Home page
B. Sturbois-Balcerzak, P. Vincent, L. Maneta-Peyret, M. Duvert, B. Satiat-Jeunemaitre, C. Cassagne, and P. Moreau
ATP-Dependent Formation of Phosphatidylserine-Rich Vesicles from the Endoplasmic Reticulum of Leek Cells
Plant Physiology, May 1, 1999; 120(1): 245 - 256.
[Abstract] [Full Text]

Home page
 Plant CellHome page
N. H. Battey, N. C. James, A. J. Greenland, and C. Brownlee
Exocytosis and Endocytosis
PLANT CELL, April 1, 1999; 11(4): 643 - 660.
[Full Text]

Home page
 Plant Physiol.Home page
H. Shin and R. M. Brown Jr.
GTPase Activity and Biochemical Characterization of a Recombinant Cotton Fiber Annexin
Plant Physiology, March 1, 1999; 119(3): 925 - 934.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1998 by the American Society of Plant Biologists