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Plant Cell, Vol. 10, 1551-1560, September 1998, Copyright © 1998, American Society of Plant Physiologists

Molecular Localization of a Redox-Modulated Process Regulating Plant Mitochondrial Electron Transport

Greg C. Vanlerberghea, Lee McIntoshb, and Justine Y. H. Yipa
a Division of Life Science and Department of Botany, University of Toronto at Scarborough, 1265 Military Trail, Scarborough, Ontario, M1C 1A4 Canada
b Michigan State University–Department of Energy Plant Research Laboratory and Biochemistry Department, Michigan State University, East Lansing, Michigan 48824

Correspondence to: Greg C. Vanlerberghe, gregv{at}scar.utoronto.ca (E-mail), 416-287-7642 (fax).

Using in organellar assays, we found that significant tobacco alternative oxidase (AOX) activity is dependent on both reduction of a putative regulatory disulfide bond and the presence of pyruvate, which may interact with a Cys sulfhydryl. This redox modulation and pyruvate activation thus may be important in determining the partitioning of electrons to AOX in vivo. To investigate these regulatory mechanisms, we generated tobacco plants expressing mutated AOX proteins. Mutation of the most N-terminal Cys residue (Cys-126) to an Ala residue produced an AOX that could not be converted to the disulfide-linked form, thus identifying this Cys residue as being responsible for redox modulation. Although this mutation might be expected to produce an AOX with constitutive high activity in the presence of pyruvate, we found it to have minimal in organellar activity in the presence of pyruvate. Nonetheless, the Cys-126 mutation did not appear to have compromised the catalytic function of AOX, given that cells expressing the protein displayed high rates of cyanide-resistant respiration in vivo. The striking difference between in vivo and in organellar results suggests that an additional mechanism(s), as yet unidentified by in organellar assays, may promote activity in vivo. Mutation of the Cys residue nearest the presumptive active site (Cys-176) to an Ala residue did not prevent disulfide bond formation or affect the ability of AOX to be stimulated by pyruvate, indicating that this Cys residue is involved in neither redox modulation nor pyruvate activation.




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