Plant Cell Illumina, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (71)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Golecki, B.
Right arrow Articles by Thompson, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Golecki, B.
Right arrow Articles by Thompson, G. A.
Agricola
Right arrow Articles by Golecki, B.
Right arrow Articles by Thompson, G. A.
Plant Cell, Vol. 11, 127-140, January 1999, Copyright © 1999, American Society of Plant Physiologists

Translocation of Structural P Proteins in the Phloem

Bettina Goleckia, Alexander Schulza, and Gary A. Thompsonb
a Botanisches Institut, Universität Kiel, Olshausenstrasse 40, D-24098 Kiel, Germany
b Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721

Correspondence to: Gary A. Thompson, garyt{at}u.arizona.edu (E-mail), 520-621-7186 (fax)

Phloem-specific proteins (P proteins) are particularly useful markers to investigate long-distance trafficking of macromolecules in plants. In this study, genus-specific molecular probes were used in combination with intergeneric grafts to reveal the presence of a pool of translocatable P protein subunits. Immunoblot analyses demonstrated that Cucurbita spp P proteins PP1 and PP2 are translocated from Cucurbita maxima stocks and accumulate in Cucumis sativus scions. Cucurbita maxima or Cucurbita ficifolia PP1 and PP2 mRNAs were not detected in Cucumis sativus scions by either RNA gel blot analysis or reverse transcription–polymerase chain reaction, indicating that the proteins, rather than transcripts, are translocated. Tissue prints of the Cucumis sativus scion, using antibodies raised against Cucurbita maxima PP1 or PP2, detected both proteins in the fascicular phloem of the stem at points distal to the graft union and in the petiole of a developing leaf, suggesting that the proteins move within the assimilate stream toward sink tissues. Cucurbita maxima PP1 was immunolocalized by light microscopy in sieve elements of the extrafascicular phloem of Cucumis sativus scions, whereas Cucurbita maxima PP2 was detected in both sieve elements and companion cells.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. Zhang, L. Sun, and F. Kragler
The Phloem-Delivered RNA Pool Contains Small Noncoding RNAs and Interferes with Translation
Plant Physiology, May 1, 2009; 150(1): 378 - 387.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M.-K. Lin, Y.-J. Lee, T. J. Lough, B. S. Phinney, and W. J. Lucas
Analysis of the Pumpkin Phloem Proteome Provides Insights into Angiosperm Sieve Tube Function
Mol. Cell. Proteomics, February 1, 2009; 8(2): 343 - 356.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. C. Pelissier, W. S. Peters, R. Collier, A. J. E. v. Bel, and M. Knoblauch
GFP Tagging of Sieve Element Occlusion (SEO) Proteins Results in Green Fluorescent Forisomes
Plant Cell Physiol., November 1, 2008; 49(11): 1699 - 1710.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M.-K. Lin, H. Belanger, Y.-J. Lee, E. Varkonyi-Gasic, K.-I. Taoka, E. Miura, B. Xoconostle-Cazares, K. Gendler, R. A. Jorgensen, B. Phinney, et al.
FLOWERING LOCUS T Protein May Act as the Long-Distance Florigenic Signal in the Cucurbits
PLANT CELL, May 1, 2007; 19(5): 1488 - 1506.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Gopinath and C. C. Kao
Replication-Independent Long-Distance Trafficking by Viral RNAs in Nicotiana benthamiana
PLANT CELL, April 1, 2007; 19(4): 1179 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Will and A. J. E. van Bel
Physical and chemical interactions between aphids and plants
J. Exp. Bot., March 1, 2006; 57(4): 729 - 737.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. l. C. Petersen, J. Hejgaard, G. A. Thompson, and A. Schulz
Cucurbit phloem serpins are graft-transmissible and appear to be resistant to turnover in the sieve element-companion cell complex
J. Exp. Bot., December 1, 2005; 56(422): 3111 - 3120.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Aoki, N. Suzui, S. Fujimaki, N. Dohmae, K. Yonekura-Sakakibara, T. Fujiwara, H. Hayashi, T. Yamaya, and H. Sakakibara
Destination-Selective Long-Distance Movement of Phloem Proteins
PLANT CELL, June 1, 2005; 17(6): 1801 - 1814.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Gomez and V. Pallas
A Long-Distance Translocatable Phloem Protein from Cucumber Forms a Ribonucleoprotein Complex In Vivo with Hop Stunt Viroid RNA
J. Virol., September 15, 2004; 78(18): 10104 - 10110.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B.-C. Yoo, F. Kragler, E. Varkonyi-Gasic, V. Haywood, S. Archer-Evans, Y. M. Lee, T. J. Lough, and W. J. Lucas
A Systemic Small RNA Signaling System in Plants
PLANT CELL, August 1, 2004; 16(8): 1979 - 2000.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Barnes, J. Bale, C. Constantinidou, P. Ashton, A. Jones, and J. Pritchard
Determining protein identity from sieve element sap in Ricinus communis L. by quadrupole time of flight (Q-TOF) mass spectrometry
J. Exp. Bot., July 1, 2004; 55(402): 1473 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
B. Hause, G. Hause, C. Kutter, O. Miersch, and C. Wasternack
Enzymes of Jasmonate Biosynthesis Occur in Tomato Sieve Elements
Plant Cell Physiol., June 15, 2003; 44(6): 643 - 648.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Dinant, A. M. Clark, Y. Zhu, F. Vilaine, J.-C. Palauqui, C. Kusiak, and G. A. Thompson
Diversity of the Superfamily of Phloem Lectins (Phloem Protein 2) in Angiosperms
Plant Physiology, January 1, 2003; 131(1): 114 - 128.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Itaya, F. Ma, Y. Qi, Y. Matsuda, Y. Zhu, G. Liang, and B. Ding
Plasmodesma-Mediated Selective Protein Traffic between "Symplasmically Isolated" Cells Probed by a Viral Movement Protein
PLANT CELL, September 1, 2002; 14(9): 2071 - 2083.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Zhu, Y. Qi, Y. Xun, R. Owens, and B. Ding
Movement of Potato Spindle Tuber Viroid Reveals Regulatory Points of Phloem-Mediated RNA Traffic
Plant Physiology, September 1, 2002; 130(1): 138 - 146.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. M. Gagne, B. P. Downes, S.-H. Shiu, A. M. Durski, and R. D. Vierstra
The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis
PNAS, August 20, 2002; 99(17): 11519 - 11524.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Asano, H. Kusano, T. Okuda, N. Kubo, H. Shimada, and K.-i. Kadowaki
Rpp16 and Rpp17, from a Common Origin, have Different Protein Characteristics but Both Genes are Predominantly Expressed in Rice Phloem Tissues
Plant Cell Physiol., June 15, 2002; 43(6): 668 - 674.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. Haywood, F. Kragler, and W. J. Lucas
Plasmodesmata: Pathways for Protein and Ribonucleoprotein Signaling
PLANT CELL, May 1, 2002; 14(90001): S303 - 325.
[Full Text] [PDF]

Home page
 DevelopmentHome page
R Ruiz-Medrano, B Xoconostle-Cazares, and W. Lucas
Phloem long-distance transport of CmNACP mRNA: implications for supracellular regulation in plants
Development, January 10, 1999; 126(20): 4405 - 4419.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
B.-C. Yoo, K. Aoki, Y. Xiang, L. R. Campbell, R. J. Hull, B. Xoconostle-Cazares, J. Monzer, J.-Y. Lee, D. E. Ullman, and W. J. Lucas
Characterization of Cucurbita maxima Phloem Serpin-1 (CmPS-1). A DEVELOPMENTALLY REGULATED ELASTASE INHIBITOR
J. Biol. Chem., November 3, 2000; 275(45): 35122 - 35128.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists