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Plant Cell, Vol. 11, 15-30, January 1999, Copyright © 1999, American Society of Plant Physiologists

Overexpression of P to Activates Defense Responses and Confers Broad Resistance

Xiaoyan Tanga, Mingtang Xiea, Young Jin Kimb, Jianmin Zhoua, Daniel F. Klessigc, and Gregory B. Martinb
a Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502
b Department of Agronomy, Purdue University, West Lafayette, Indiana 47907-1150
c Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, P.O. Box 759, Piscataway, New Jersey 08854-8020

Correspondence to: Xiaoyan Tang, xtang{at}plantpath.ksu.edu (E-mail), 785-532-5692 (fax)

The tomato disease resistance (R) gene Pto specifies race-specific resistance to the bacterial pathogen Pseudomonas syringae pv tomato carrying the avrPto gene. Pto encodes a serine/threonine protein kinase that is postulated to be activated by a physical interaction with the AvrPto protein. Here, we report that overexpression of Pto in tomato activates defense responses in the absence of the Pto–AvrPto interaction. Leaves of three transgenic tomato lines carrying the cauliflower mosaic virus 35S::Pto transgene exhibited microscopic cell death, salicylic acid accumulation, and increased expression of pathogenesis-related genes. Cell death in these plants was limited to palisade mesophyll cells and required light for induction. Mesophyll cells of 35S::Pto plants showed the accumulation of autofluorescent compounds, callose deposition, and lignification. When inoculated with P. s. tomato without avrPto, all three 35S::Pto lines displayed significant resistance and supported less bacterial growth than did nontransgenic lines. Similarly, the 35S::Pto lines also were more resistant to Xanthomonas campestris pv vesicatoria and Cladosporium fulvum. These results demonstrate that defense responses and general resistance can be activated by the overexpression of an R gene.




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