Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (90)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meissner, R. C.
Right arrow Articles by Bevan, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meissner, R. C.
Right arrow Articles by Bevan, M.
Agricola
Right arrow Articles by Meissner, R. C.
Right arrow Articles by Bevan, M.
Plant Cell, Vol. 11, 1827-1840, October 1999, Copyright © 1999, American Society of Plant Physiologists

GENOMICS ARTICLE

Function Search in a Large Transcription Factor Gene Family in Arabidopsis: Assessing the Potential of Reverse Genetics to Identify Insertional Mutations in R2R3 MYB Genes

Ruth C. Meissnera, Hailing Jinb, Eleonora Cominellic, Marten Denekampd, Antonio Fuertese, Raffaella Grecoc, Harald D. Kranzf, Steven Penfielda, Katia Petronic, Ana Urzainquie, Cathie Martinb, Javier Paz-Arese, Sjef Smeekensd, Chiara Tonellic, Bernd Weisshaarf, Elvira Baumanng, Victor Klimyukh, Sylvestre Marillonneth, Kanu Patelh, Elly Speulmani, Alain F. Tissierh, David Bouchezj, Jonathan J. D. Jonesh, Andy Pereirai, Ellen Wismang, and Michael Bevana
a John Innes Centre, Department of Molecular Genetics, Colney Lane, Norwich Research Park, Norwich NR4 7UH, United Kingdom
b John Innes Centre, Genetics Department, Colney Lane, Norwich Research Park, Norwich NR4 7UH, United Kingdom
c Dipartimento di Genetica e di Biologia dei Microrganismi, Universita degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
d Department of Molecular Cell Biology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
e Centro Nacional de Biotecnologia–Consejo Superior de Investigaciones, Campus de Cantoblanco, 28049 Madrid, Spain
f Max-Planck Institut für Züchtungsforschung, Abteilung Biochemie, Carl-von-Linne-Weg 10, 50829 Köln, Germany
g Max-Planck Institut für Züchtungsforschung, Abteilung Saedler, Carl-von-Linne-Weg 10, 50829 Köln, Germany
h John Innes Centre, Sainsbury Laboratory, Colney Lane, Norwich Research Park, Norwich NR4 7UH, United Kingdom
i CPRO-DLO, Department of Molecular Biology, Droevendaalsesteeg 1, 6700 AA Wageningen, The Netherlands
j Institut National de la Recherche Agronomique, Centre de Versailles, Laboratoire de Biologie Cellulaire, Route de Saint-Cyr, 78026 Versailles Cedex, France

Correspondence to: Michael Bevan, bevan{at}bbsrc.ac.uk (E-mail), 44-1603-505725 (fax)

More than 92 genes encoding MYB transcription factors of the R2R3 class have been described in Arabidopsis. The functions of a few members of this large gene family have been described, indicating important roles for R2R3 MYB transcription factors in the regulation of secondary metabolism, cell shape, and disease resistance, and in responses to growth regulators and stresses. For the majority of the genes in this family, however, little functional information is available. As the first step to characterizing these genes functionally, the sequences of >90 family members, and the map positions and expression profiles of >60 members, have been determined previously. An important second step in the functional analysis of the MYB family, through a process of reverse genetics that entails the isolation of insertion mutants, is described here. For this purpose, a variety of gene disruption resources has been used, including T-DNA–insertion populations and three distinct populations that harbor transposon insertions. We report the isolation of 47 insertions into 36 distinct MYB genes by screening a total of 73 genes. These defined insertion lines will provide the foundation for subsequent detailed functional analyses for the assignment of specific functions to individual members of the R2R3 MYB gene family.




This article has been cited by other articles:


Home page
 J Exp BotHome page
K. Petroni, G. Falasca, V. Calvenzani, D. Allegra, C. Stolfi, L. Fabrizi, M. M. Altamura, and C. Tonelli
The AtMYB11 gene from Arabidopsis is expressed in meristematic cells and modulates growth in planta and organogenesis in vitro
J. Exp. Bot., April 1, 2008; 59(6): 1201 - 1213.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Jung, J. S. Seo, S. W. Han, Y. J. Koo, C. H. Kim, S. I. Song, B. H. Nahm, Y. D. Choi, and J.-J. Cheong
Overexpression of AtMYB44 Enhances Stomatal Closure to Confer Abiotic Stress Tolerance in Transgenic Arabidopsis
Plant Physiology, February 1, 2008; 146(2): 623 - 635.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. J. Conrad, L. Bai, K. Ahern, K. Dusinberre, D. P. Kane, and T. P. Brutnell
State II Dissociation Element Formation Following Activator Excision in Maize
Genetics, October 1, 2007; 177(2): 737 - 747.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Khanna, Y. Shen, G. Toledo-Ortiz, E. A. Kikis, H. Johannesson, Y.-S. Hwang, and P. H. Quail
Functional Profiling Reveals That Only a Small Number of Phytochrome-Regulated Early-Response Genes in Arabidopsis Are Necessary for Optimal Deetiolation
PLANT CELL, September 1, 2006; 18(9): 2157 - 2171.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. J. Conrad and T. P. Brutnell
Ac-Immobilized, a Stable Source of Activator Transposase That Mediates Sporophytic and Gametophytic Excision of Dissociation Elements in Maize
Genetics, December 1, 2005; 171(4): 1999 - 2012.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Mehrtens, H. Kranz, P. Bednarek, and B. Weisshaar
The Arabidopsis Transcription Factor MYB12 Is a Flavonol-Specific Regulator of Phenylpropanoid Biosynthesis
Plant Physiology, June 1, 2005; 138(2): 1083 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Okushima, P. J. Overvoorde, K. Arima, J. M. Alonso, A. Chan, C. Chang, J. R. Ecker, B. Hughes, A. Lui, D. Nguyen, et al.
Functional Genomic Analysis of the AUXIN RESPONSE FACTOR Gene Family Members in Arabidopsis thaliana: Unique and Overlapping Functions of ARF7 and ARF19
PLANT CELL, February 1, 2005; 17(2): 444 - 463.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. M. Kolkman, L. J. Conrad, P. R. Farmer, K. Hardeman, K. R. Ahern, P. E. Lewis, R. J. H. Sawers, S. Lebejko, P. Chomet, and T. P. Brutnell
Distribution of Activator (Ac) Throughout the Maize Genome for Use in Regional Mutagenesis
Genetics, February 1, 2005; 169(2): 981 - 995.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. An, S. Lee, S.-H. Kim, and S.-R. Kim
Molecular Genetics Using T-DNA in Rice
Plant Cell Physiol., January 15, 2005; 46(1): 14 - 22.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Matsui, K. Hiratsu, T. Koyama, H. Tanaka, and M. Ohme-Takagi
A Chimeric AtMYB23 Repressor Induces Hairy Roots, Elongation of Leaves and Stems, and Inhibition of the Deposition of Mucilage on Seed Coats in Arabidopsis
Plant Cell Physiol., January 15, 2005; 46(1): 147 - 155.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Lee, J. Kim, J.-S. Son, J. Nam, D.-H. Jeong, K. Lee, S. Jang, J. Yoo, J. Lee, D.-Y. Lee, et al.
Systematic Reverse Genetic Screening of T-DNA Tagged Genes in Rice for Functional Genomic Analyses: MADS-box Genes as a Test Case
Plant Cell Physiol., December 15, 2003; 44(12): 1403 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Elomaa, A. Uimari, M. Mehto, V. A. Albert, R. A.E. Laitinen, and T. H. Teeri
Activation of Anthocyanin Biosynthesis in Gerbera hybrida (Asteraceae) Suggests Conserved Protein-Protein and Protein-Promoter Interactions between the Anciently Diverged Monocots and Eudicots
Plant Physiology, December 1, 2003; 133(4): 1831 - 1842.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S. An, S. Park, D.-H. Jeong, D.-Y. Lee, H.-G. Kang, J.-H. Yu, J. Hur, S.-R. Kim, Y.-H. Kim, M. Lee, et al.
Generation and Analysis of End Sequence Database for T-DNA Tagging Lines in Rice
Plant Physiology, December 1, 2003; 133(4): 2040 - 2047.
[Abstract] [Full Text]

Home page
 Plant CellHome page
M. Vandenbussche, J. Zethof, E. Souer, R. Koes, G. B. Tornielli, M. Pezzotti, S. Ferrario, G. C. Angenent, and T. Gerats
Toward the Analysis of the Petunia MADS Box Gene Family by Reverse and Forward Transposon Insertion Mutagenesis Approaches: B, C, and D Floral Organ Identity Functions Require SEPALLATA-Like MADS Box Genes in Petunia
PLANT CELL, November 1, 2003; 15(11): 2680 - 2693.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. R. Muskett, L. Clissold, A. Marocco, P. S. Springer, R. Martienssen, and C. Dean
A Resource of Mapped Dissociation Launch Pads for Targeted Insertional Mutagenesis in the Arabidopsis Genome
Plant Physiology, June 1, 2003; 132(2): 506 - 516.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. A. Dixon and L. W. Sumner
Legume Natural Products: Understanding and Manipulating Complex Pathways for Human and Animal Health
Plant Physiology, March 1, 2003; 131(3): 878 - 885.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. J. Schultz, M. P. Rumsewicz, K. L. Johnson, B. J. Jones, Y. M. Gaspar, and A. Bacic
Using Genomic Resources to Guide Research Directions. The Arabinogalactan Protein Gene Family as a Test Case
Plant Physiology, August 1, 2002; 129(4): 1448 - 1463.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. H. Cheong, H.-S. Chang, R. Gupta, X. Wang, T. Zhu, and S. Luan
Transcriptional Profiling Reveals Novel Interactions between Wounding, Pathogen, Abiotic Stress, and Hormonal Responses in Arabidopsis
Plant Physiology, June 1, 2002; 129(2): 661 - 677.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Samson, V. Brunaud, S. Balzergue, B. Dubreucq, L. Lepiniec, G. Pelletier, M. Caboche, and A. Lecharny
FLAGdb/FST: a database of mapped flanking insertion sites (FSTs) of Arabidopsis thaliana T-DNA transformants
Nucleic Acids Res., January 1, 2002; 30(1): 94 - 97.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. J. Budziszewski, S. P. Lewis, L. W. Glover, J. Reineke, G. Jones, L. S. Ziemnik, J. Lonowski, B. Nyfeler, G. Aux, Q. Zhou, et al.
Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular Cloning
Genetics, December 1, 2001; 159(4): 1765 - 1778.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Penfield, R. C. Meissner, D. A. Shoue, N. C. Carpita, and M. W. Bevan
MYB61 Is Required for Mucilage Deposition and Extrusion in the Arabidopsis Seed Coat
PLANT CELL, December 1, 2001; 13(12): 2777 - 2791.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Nesi, C. Jond, I. Debeaujon, M. Caboche, and L. Lepiniec
The Arabidopsis TT2 Gene Encodes an R2R3 MYB Domain Protein That Acts as a Key Determinant for Proanthocyanidin Accumulation in Developing Seed
PLANT CELL, September 1, 2001; 13(9): 2099 - 2114.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Mahalingam and N. Fedoroff
Screening insertion libraries for mutations in many genes simultaneously using DNA microarrays
PNAS, June 19, 2001; 98(13): 7420 - 7425.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Scheres and J. Browse
Playing with Arabidopsis
Plant Physiology, June 1, 2001; 126(2): 468 - 470.
[Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Hiraga, K. Sasaki, H. Ito, Y. Ohashi, and H. Matsui
A Large Family of Class III Plant Peroxidases
Plant Cell Physiol., May 1, 2001; 42(5): 462 - 468.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Kovtun, W.-L. Chiu, G. Tena, and J. Sheen
From the Cover: Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants
PNAS, March 14, 2000; 97(6): 2940 - 2945.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. B. Smith
Proteomics: Broad Strokes of Expressionism?
PLANT CELL, March 1, 2000; 12(3): 303 - 304.
[Full Text]

Home page
 Plant CellHome page
A. F. Tissier, S. Marillonnet, V. Klimyuk, K. Patel, M. A. Torres, G. Murphy, and J. D. G. Jones
Multiple Independent Defective Suppressor-mutator Transposon Insertions in Arabidopsis: A Tool for Functional Genomics
PLANT CELL, October 1, 1999; 11(10): 1841 - 1852.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists