Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (47)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshizumi, T.
Right arrow Articles by Matsui, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshizumi, T.
Right arrow Articles by Matsui, M.
Agricola
Right arrow Articles by Yoshizumi, T.
Right arrow Articles by Matsui, M.
Plant Cell, Vol. 11, 1883-1896, October 1999, Copyright © 1999, American Society of Plant Physiologists

An Arabidopsis Cell Cycle –Dependent Kinase-Related Gene, CDC2b, Plays a Role in Regulating Seedling Growth in Darkness

Takeshi Yoshizumia,b, Noriko Nagatac, Hiroaki Shimadab, and Minami Matsuia
a Laboratory for Photoperception and Signal Transduction, Frontier Research Program, Institute of Physical and Chemical Research (RIKEN) 2-1, Hirosawa, Wako-shi, Saitama 351-0198, Japan
b Department of Biological Science, Science University of Tokyo, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
c Laboratory of Plant Functions, Institute of Physical and Chemical Research (RIKEN) 2-1, Hirosawa, Wako-shi, Saitama 351-0198, Japan

Correspondence to: Minami Matsui, minami{at}postman.riken.go.jp (E-mail), 81-48-462-9405 (fax)

The Arabidopsis CDC2b gene has been defined as a plant-specific cell cycle–dependent kinase-related gene, although it lacks the conserved cyclin binding motif, and its exact function is not known. Here, we report that in etiolated seedlings, the expression of the CDC2b gene is correlated with elongation rate of the hypocotyl. Inhibition of CDC2b gene expression by using an inducible antisense construct resulted in short-hypocotyl and open-cotyledon phenotypes when transgenic seedlings were grown in the dark. The severity of these phenotypes in dark-grown seedlings could be correlated with the level of the antisense gene expression. The short hypocotyl of seedlings underexpressing CDC2b was a result of inhibition of cell elongation rather than a reduction in cell number, whereas in cotyledons, inhibition of CDC2b expression resulted in large, open cotyledons with amyloplasts rather than etioplasts. Although the nuclear DNA was less compact in the antisense hypocotyl cells, DNA content and endoreduplication were not affected. Cell division of the shoot apical meristem also was not affected by antisense expression. The short-hypocotyl phenotype of these transgenic plants was partially rescued by the addition of brassinolide. Brassinolide can only induce CDC2b expression in darkness. These results suggest a role for the CDC2b gene in seedling growth via regulation of hypocotyl cell elongation and cotyledon cell development.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. C. Day, R. P. Herridge, B. A. Ambrose, and R. C. Macknight
Transcriptome Analysis of Proliferating Arabidopsis Endosperm Reveals Biological Implications for the Control of Syncytial Division, Cytokinin Signaling, and Gene Expression Regulation
Plant Physiology, December 1, 2008; 148(4): 1964 - 1984.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Q. Fu, W. H. Mao, K. Shi, Y. H. Zhou, T. Asami, and J. Q. Yu
A role of brassinosteroids in early fruit development in cucumber
J. Exp. Bot., June 1, 2008; 59(9): 2299 - 2308.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. K. Cho, H. S. Chung, M. Y. Ryu, M. J. Park, M. M. Lee, Y.-Y. Bahk, J. Kim, H. S. Pai, and W. T. Kim
Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog
Plant Physiology, December 1, 2006; 142(4): 1664 - 1682.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Yoshizumi, Y. Tsumoto, T. Takiguchi, N. Nagata, Y. Y. Yamamoto, M. Kawashima, T. Ichikawa, M. Nakazawa, N. Yamamoto, and M. Matsui
INCREASED LEVEL OF POLYPLOIDY1, a Conserved Repressor of CYCLINA2 Transcription, Controls Endoreduplication in Arabidopsis
PLANT CELL, October 1, 2006; 18(10): 2452 - 2468.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. Boudolf, K. Vlieghe, G. T.S. Beemster, Z. Magyar, J. A. T. Acosta, S. Maes, E. Van Der Schueren, D. Inze, and L. De Veylder
The Plant-Specific Cyclin-Dependent Kinase CDKB1;1 and Transcription Factor E2Fa-DPa Control the Balance of Mitotically Dividing and Endoreduplicating Cells in Arabidopsis
PLANT CELL, October 1, 2004; 16(10): 2683 - 2692.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Ramirez-Parra, M. A. Lopez-Matas, C. Frundt, and C. Gutierrez
Role of an Atypical E2F Transcription Factor in the Control of Arabidopsis Cell Growth and Differentiation
PLANT CELL, September 1, 2004; 16(9): 2350 - 2363.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. Boudolf, R. Barroco, J. d. A. Engler, A. Verkest, T. Beeckman, M. Naudts, D. Inze, and L. De Veylder
B1-Type Cyclin-Dependent Kinases Are Essential for the Formation of Stomatal Complexes in Arabidopsis thaliana
PLANT CELL, April 1, 2004; 16(4): 945 - 955.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Takase, M. Nakazawa, A. Ishikawa, K. Manabe, and M. Matsui
DFL2, a New Member of the Arabidopsis GH3 Gene Family, is Involved in Red Light-Specific Hypocotyl Elongation
Plant Cell Physiol., October 15, 2003; 44(10): 1071 - 1080.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. M. Friedrichsen, J. Nemhauser, T. Muramitsu, J. N. Maloof, J. Alonso, J. R. Ecker, M. Furuya, and J. Chory
Three Redundant Brassinosteroid Early Response Genes Encode Putative bHLH Transcription Factors Required for Normal Growth
Genetics, November 1, 2002; 162(3): 1445 - 1456.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. T.S. Beemster, K. De Vusser, E. De Tavernier, K. De Bock, and D. Inze
Variation in Growth Rate between Arabidopsis Ecotypes Is Correlated with Cell Division and A-Type Cyclin-Dependent Kinase Activity
Plant Physiology, June 1, 2002; 129(2): 854 - 864.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. J. Bishop and C. Koncz
Brassinosteroids and Plant Steroid Hormone Signaling
PLANT CELL, May 1, 2002; 14(90001): S97 - 110.
[Full Text] [PDF]

Home page
 Plant CellHome page
M. M. Castellano, J. C. del Pozo, E. Ramirez-Parra, S. Brown, and C. Gutierrez
Expression and Stability of Arabidopsis CDC6 Are Associated with Endoreplication
PLANT CELL, December 1, 2001; 13(12): 2671 - 2686.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Joubes, M. Lemaire-Chamley, F. Delmas, J. Walter, M. Hernould, A. Mouras, P. Raymond, and C. Chevalier
A New C-Type Cyclin-Dependent Kinase from Tomato Expressed in Dividing Tissues Does Not Interact with Mitotic and G1 Cyclins
Plant Physiology, August 1, 2001; 126(4): 1403 - 1415.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Lally, P. Ingmire, H.-Y. Tong, and Z.-H. He
Antisense Expression of a Cell Wall-Associated Protein Kinase, WAK4, Inhibits Cell Elongation and Alters Morphology
PLANT CELL, June 1, 2001; 13(6): 1317 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. J. Bishop and T. Yokota
Plants Steroid Hormones, Brassinosteroids: Current Highlights of Molecular Aspects on their Synthesis/Metabolism, Transport, Perception and Response
Plant Cell Physiol., February 1, 2001; 42(2): 114 - 120.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Porceddu, H. Stals, J.-P. Reichheld, G. Segers, L. De Veylder, R. de Pinho Barroco, P. Casteels, M. Van Montagu, D. Inze, and V. Mironov
A Plant-specific Cyclin-dependent Kinase Is Involved in the Control of G2/M Progression in Plants
J. Biol. Chem., September 21, 2001; 276(39): 36354 - 36360.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C.-F. Chuang and E. M. Meyerowitz
Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana
PNAS, April 25, 2000; 97(9): 4985 - 4990.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists