Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (47)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stacey, M. G.
Right arrow Articles by von Arnim, A. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stacey, M. G.
Right arrow Articles by von Arnim, A. G.
Agricola
Right arrow Articles by Stacey, M. G.
Right arrow Articles by von Arnim, A. G.
Plant Cell, Vol. 11, 349-364, March 1999, Copyright © 1999, American Society of Plant Physiologists

Discrete Domains Mediate the Light-Responsive Nuclear and Cytoplasmic Localization of Arabidopsis COP1

Minviluz G. Staceya, Stephanie N. Hicksa, and Albrecht G. von Arnima
a Department of Botany, University of Tennessee, HBB 437, Knoxville, Tennessee 37996-1100

Correspondence to: Albrecht G. von Arnim, vonarnim{at}utk.edu (E-mail), 423-974-0978 (fax)

The Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) protein plays a critical role in the repression of photomorphogenesis during Arabidopsis seedling development. We investigated the control of COP1 partitioning between nucleus and cytoplasm, which has been implicated in the regulation of COP1 activity, by using fusion proteins between COP1 and ß-glucuronidase or the green fluorescent protein. Transient expression assays using onion epidermal cells and data from hypocotyl cells of stably transformed Arabidopsis demonstrated that COP1 carries a single, bipartite nuclear localization signal that functions independently of light. Nuclear exclusion was mediated by a novel and distinct signal, bordering the zinc-finger and coiled-coil motifs, that was able to redirect a heterologous nuclear protein to the cytoplasm. The cytoplasmic localization signal functioned in a light-independent manner. Light regulation of nuclear localization was reconstituted by combining the individual domains containing the nuclear localization signal and the cytoplasmic localization signal; the WD-40 repeat domain of COP1 was not required. However, phenotypic analysis of transgenic seedlings suggested that the constitutively nuclear-localized WD-40 repeat domain was able to mimic aspects of COP1 function, as indicated by exaggerated hypocotyl elongation under light conditions.




This article has been cited by other articles:


Home page
 J Exp BotHome page
Y. Lee, H.-S. Lee, J.-S. Lee, S.-K. Kim, and S.-H. Kim
Hormone- and light-regulated nucleocytoplasmic transport in plants: current status
J. Exp. Bot., September 1, 2008; 59(12): 3229 - 3245.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Zhao, W. Zhang, Y. Zhao, X. Gong, L. Guo, G. Zhu, X. Wang, Z. Gong, K. S. Schumaker, and Y. Guo
SAD2, an Importin -Like Protein, Is Required for UV-B Response in Arabidopsis by Mediating MYB4 Nuclear Trafficking
PLANT CELL, November 1, 2007; 19(11): 3805 - 3818.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J.-Y. Lee, J. Colinas, J. Y. Wang, D. Mace, U. Ohler, and P. N. Benfey
Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis roots
PNAS, April 11, 2006; 103(15): 6055 - 6060.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T.-H. Kim, B.-H. Kim, A. Yahalom, D. A. Chamovitz, and A. G. von Arnim
Translational Regulation via 5' mRNA Leader Sequences Revealed by Mutational Analysis of the Arabidopsis Translation Initiation Factor Subunit eIF3h
PLANT CELL, December 1, 2004; 16(12): 3341 - 3356.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Subramanian, B.-H. Kim, N. N. Lyssenko, X. Xu, C. H. Johnson, and A. G. von Arnim
The Arabidopsis repressor of light signaling, COP1, is regulated by nuclear exclusion: Mutational analysis by bioluminescence resonance energy transfer
PNAS, April 27, 2004; 101(17): 6798 - 6802.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Nakagawa and Y. Komeda
Flowering of Arabidopsis cop1 Mutants in Darkness
Plant Cell Physiol., April 15, 2004; 45(4): 398 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Dieterle, C. Buche, E. Schafer, and T. Kretsch
Characterization of a Novel Non-Constitutive Photomorphogenic cop1 Allele
Plant Physiology, December 1, 2003; 133(4): 1557 - 1564.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
M. Holm, L.-G. Ma, L.-J. Qu, and X.-W. Deng
Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis
Genes & Dev., May 15, 2002; 16(10): 1247 - 1259.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Mano, C. Nakamori, M. Hayashi, A. Kato, M. Kondo, and M. Nishimura
Distribution and Characterization of Peroxisomes in Arabidopsis by Visualization with GFP: Dynamic Morphology and Actin-Dependent Movement
Plant Cell Physiol., March 1, 2002; 43(3): 331 - 341.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. P. Kastenmayer and P. J. Green
Novel features of the XRN-family in Arabidopsis: Evidence that AtXRN4, one of several orthologs of nuclear Xrn2p/Rat1p, functions in the cytoplasm
PNAS, December 5, 2000; 97(25): 13985 - 13990.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
E. B. Blancaflor and S. Gilroy
Plant cell biology in the new millennium: new tools and new insights
Am. J. Botany, November 1, 2000; 87(11): 1547 - 1560.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
M. G. Stacey, O. R. Kopp, T.-H. Kim, and A. G. von Arnim
Modular Domain Structure of Arabidopsis COP1. Reconstitution of Activity by Fragment Complementation and Mutational Analysis of a Nuclear Localization Signal in Planta
Plant Physiology, November 1, 2000; 124(3): 979 - 990.
[Abstract] [Full Text]

Home page
 Plant CellHome page
J. A. Sullivan and J. C. Gray
The Pea light-independent photomorphogenesis1 Mutant Results from Partial Duplication of COP1 Generating an Internal Promoter and Producing Two Distinct Transcripts
PLANT CELL, October 1, 2000; 12(10): 1927 - 1938.
[Abstract] [Full Text]

Home page
 Plant CellHome page
D. Cao, Y. Lin, and C.-L. Cheng
Genetic Interactions between the Chlorate-Resistant Mutant cr 8 8 and the Photomorphogenic Mutants cop1 and hy5
PLANT CELL, February 1, 2000; 12(2): 199 - 210.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
K. U. Torii, C. D. Stoop-Myer, H. Okamoto, J. E. Coleman, M. Matsui, and X. W. Deng
The RING Finger Motif of Photomorphogenic Repressor COP1 Specifically Interacts with the RING-H2 Motif of a Novel Arabidopsis Protein
J. Biol. Chem., September 24, 1999; 274(39): 27674 - 27681.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. G. Stacey and A. G. von Arnim
A Novel Motif Mediates the Targeting of the Arabidopsis COP1 Protein to Subnuclear Foci
J. Biol. Chem., September 17, 1999; 274(38): 27231 - 27236.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-J. Jiang, K. Shoji, R. Matsuki, A. Baba, N. Inagaki, H. Ban, T. Iwasaki, N. Imamoto, Y. Yoneda, X.-W. Deng, et al.
Molecular Cloning of a Novel Importin alpha Homologue from Rice, by Which Constitutive Photomorphogenic 1 (COP1) Nuclear Localization Signal (NLS)-Protein Is Preferentially Nuclear Imported
J. Biol. Chem., March 16, 2001; 276(12): 9322 - 9329.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists