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Plant Cell, Vol. 11, 471-484, March 1999, Copyright © 1999, American Society of Plant Physiologists

Stochastic and Nonstochastic Post-Transcriptional Silencing of Chitinase and ß-1,3-Glucanase Genes Involves Increased RNA Turnover—Possible Role for Ribosome-Independent RNA Degradation

Hauke Holtorfa, Hanspeter Schöba, Christian Kunza, Rosa Waldvogela, and Frederick Meins, Jr.a
a Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland

Correspondence to: Frederick Meins, Jr., at Maulbeerstrasse 66, CH-4058 Basel, Switzerland., meins{at}fmi.ch (E-mail), 41-61-697-3976 (fax)

Stochastic and nonstochastic post-transcriptional gene silencing (PTGS) in Nicotiana sylvestris plants carrying tobacco class I chitinase (CHN) and ß-1,3-glucanase transgenes differs in incidence, stability, and pattern of expression. Measurements with inhibitors of RNA synthesis (cordycepin, actinomycin D, and {alpha}-amanitin) showed that both forms of PTGS are associated with increased sequence-specific degradation of transcripts, suggesting that increased RNA turnover may be a general feature of PTGS. The protein synthesis inhibitors cycloheximide and verrucarin A did not inhibit degradation of CHN RNA targeted for PTGS, confirming that PTGS-related RNA degradation does not depend on ongoing protein synthesis. Because verrucarin A, unlike cycloheximide, dissociates mRNA from ribosomes, our results also suggest that ribosome-associated RNA degradation pathways may not be involved in CHN PTGS.




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