Plant Cell Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (72)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Franklin, A. E.
Right arrow Articles by Cande, W. Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Franklin, A. E.
Right arrow Articles by Cande, W. Z.
Agricola
Right arrow Articles by Franklin, A. E.
Right arrow Articles by Cande, W. Z.
Plant Cell, Vol. 11, 809-824, May 1999, Copyright © 1999, American Society of Plant Physiologists

Three-Dimensional Microscopy of the Rad51 Recombination Protein during Meiotic Prophase

Amie E. Franklina, John McElverb, Ivana Sunjevaricc, Rodney Rothsteinc, Ben Bowend, and W. Zacheus Candea
a Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720
b Novartis Agribusiness Biotechnology Research Inc., 3054 Cornwallis Road, Research Triangle Park, North Carolina 27709
c Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032-2704
d Lynx Therapeutics, Inc., Hayward, California 94545

Correspondence to: Amie E. Franklin, franklin{at}candelab.berkeley.edu (E-mail), 510-643-6791 (fax)

An open question in meiosis is whether the Rad51 recombination protein functions solely in meiotic recombination or whether it is also involved in the chromosome homology search. To address this question, we have performed three-dimensional high-resolution immunofluorescence microscopy to visualize native Rad51 structures in maize male meiocytes. Maize has two closely related RAD51 genes that are expressed at low levels in differentiated tissues and at higher levels in mitotic and meiotic tissues. Cells and nuclei were specially fixed and embedded in polyacrylamide to maintain both native chromosome structure and the three dimensionality of the specimens. Analysis of Rad51 in maize meiocytes revealed that when chromosomes condense during leptotene, Rad51 is diffuse within the nucleus. Rad51 foci form on the chromosomes at the beginning of zygotene and rise to ~500 per nucleus by mid-zygotene when chromosomes are pairing and synapsing. During chromosome pairing, we consistently found two contiguous Rad51 foci on paired chromosomes. These paired foci may identify the sites where DNA sequence homology is being compared. During pachytene, the number of Rad51 foci drops to seven to 22 per nucleus. This higher number corresponds approximately to the number of chiasmata in maize meiosis. These observations are consistent with a role for Rad51 in the homology search phase of chromosome pairing in addition to its known role in meiotic recombination.




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
K. Wang, D. Tang, M. Wang, J. Lu, H. Yu, J. Liu, B. Qian, Z. Gong, X. Wang, J. Chen, et al.
MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice
J. Cell Sci., June 15, 2009; 122(12): 2055 - 2063.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. P. Pawlowski, C.-J. R. Wang, I. N. Golubovskaya, J. M. Szymaniak, L. Shi, O. Hamant, T. Zhu, L. Harper, W. F. Sheridan, and W. Z. Cande
Maize AMEIOTIC1 is essential for multiple early meiotic processes and likely required for the initiation of meiosis
PNAS, March 3, 2009; 106(9): 3603 - 3608.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
D. Phillips, E. I. Mikhailova, L. Timofejeva, J. L. Mitchell, O. Osina, S. P. Sosnikhina, R. N. Jones, and G. Jenkins
Dissecting Meiosis of Rye using Translational Proteomics
Ann. Bot., April 1, 2008; 101(6): 873 - 880.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. Li, T.-J. Wen, and P. S. Schnable
Role of RAD51 in the Repair of MuDR-Induced Double-Strand Breaks in Maize (Zea mays L.)
Genetics, January 1, 2008; 178(1): 57 - 66.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Ronceret, C. G. Bozza, and W. P. Pawlowski
Naughty Behavior of Maize Minichromosomes in Meiosis
PLANT CELL, December 1, 2007; 19(12): 3835 - 3837.
[Full Text] [PDF]

Home page
 GeneticsHome page
J. Li, L. C. Harper, I. Golubovskaya, C. R. Wang, D. Weber, R. B. Meeley, J. McElver, B. Bowen, W. Z. Cande, and P. S. Schnable
Functional Analysis of Maize RAD51 in Meiosis and Double-Strand Break Repair
Genetics, July 1, 2007; 176(3): 1469 - 1482.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Pereira, M Todorova, X Cai, C. Makaroff, R. Emery, and B. Moffatt
Methyl recycling activities are co-ordinately regulated during plant development
J. Exp. Bot., March 1, 2007; 58(5): 1083 - 1098.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
I. N. Golubovskaya, O. Hamant, L. Timofejeva, C.-J. R. Wang, D. Braun, R. Meeley, and W. Z. Cande
Alleles of afd1 dissect REC8 functions during meiotic prophase I
J. Cell Sci., August 15, 2006; 119(16): 3306 - 3315.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Chelysheva, S. Diallo, D. Vezon, G. Gendrot, N. Vrielynck, K. Belcram, N. Rocques, A. Marquez-Lema, A. M. Bhatt, C. Horlow, et al.
AtREC8 and AtSCC3 are essential to the monopolar orientation of the kinetochores during meiosis
J. Cell Sci., October 15, 2005; 118(20): 4621 - 4632.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Calvente, A. Viera, J. Page, M. T. Parra, R. Gomez, J. A. Suja, J. S. Rufas, and J. L. Santos
DNA double-strand breaks and homology search: inferences from a species with incomplete pairing and synapsis
J. Cell Sci., July 1, 2005; 118(13): 2957 - 2963.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Li, X. Yang, Z. Lin, L. Timofejeva, R. Xiao, C. A. Makaroff, and H. Ma
The AtRAD51C Gene Is Required for Normal Meiotic Chromosome Synapsis and Double-Stranded Break Repair in Arabidopsis
Plant Physiology, June 1, 2005; 138(2): 965 - 976.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Sanchez-Moran, G. H. Jones, F. C. H. Franklin, and J. L. Santos
A Puromycin-Sensitive Aminopeptidase Is Essential for Meiosis in Arabidopsis thaliana
PLANT CELL, November 1, 2004; 16(11): 2895 - 2909.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. Li, C. Chen, U. Markmann-Mulisch, L. Timofejeva, E. Schmelzer, H. Ma, and B. Reiss
The Arabidopsis AtRAD51 gene is dispensable for vegetative development but required for meiosis
PNAS, July 20, 2004; 101(29): 10596 - 10601.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
W. P. Pawlowski, I. N. Golubovskaya, L. Timofejeva, R. B. Meeley, W. F. Sheridan, and W. Z. Cande
Coordination of Meiotic Recombination, Pairing, and Synapsis by PHS1
Science, January 2, 2004; 303(5654): 89 - 92.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. P. Pawlowski, I. N. Golubovskaya, and W. Z. Cande
Altered Nuclear Distribution of Recombination Protein RAD51 in Maize Mutants Suggests the Involvement of RAD51 in Meiotic Homology Recognition
PLANT CELL, August 1, 2003; 15(8): 1807 - 1816.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
X. Yang, C. A. Makaroff, and H. Ma
The Arabidopsis MALE MEIOCYTE DEATH1 Gene Encodes a PHD-Finger Protein That Is Required for Male Meiosis
PLANT CELL, June 1, 2003; 15(6): 1281 - 1295.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
B. Agashe, C. K. Prasad, and I. Siddiqi
Identification and analysis of DYAD: a gene required for meiotic chromosome organisation and female meiotic progression in Arabidopsis
Development, March 10, 2003; 129(16): 3935 - 3943.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Schwarzacher
Meiosis, recombination and chromosomes: a review of gene isolation and fluorescent in situ hybridization data in plants
J. Exp. Bot., January 1, 2003; 54(380): 11 - 23.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
I. N. Golubovskaya, L. C. Harper, W. P. Pawlowski, D. Schichnes, and W. Z. Cande
The pam1 Gene Is Required for Meiotic Bouquet Formation and Efficient Homologous Synapsis in Maize (Zea mays L.)
Genetics, December 1, 2002; 162(4): 1979 - 1993.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. L. Peoples, E. Dean, O. Gonzalez, L. Lambourne, and S. M. Burgess
Close, stable homolog juxtaposition during meiosis in budding yeast is dependent on meiotic recombination, occurs independently of synapsis, and is distinct from DSB-independent pairing contacts
Genes & Dev., July 1, 2002; 16(13): 1682 - 1695.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
P. M. Carlton and W. Z. Cande
Telomeres act autonomously in maize to organize the meiotic bouquet from a semipolarized chromosome orientation
J. Cell Biol., April 15, 2002; 157(2): 231 - 242.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. P. Copenhaver, E. A. Housworth, and F. W. Stahl
Crossover Interference in Arabidopsis
Genetics, April 1, 2002; 160(4): 1631 - 1639.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
U. Markmann-Mulisch, M. Z. Hadi, K. Koepchen, J. C. Alonso, V. E. A. Russo, J. Schell, and B. Reiss
The organization of Physcomitrella patensRAD51 genes is unique among eukaryotic organisms
PNAS, March 5, 2002; 99(5): 2959 - 2964.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. K. Anderson, K. D. Hooker, and S. M. Stack
The Distribution of Early Recombination Nodules on Zygotene Bivalents From Plants
Genetics, November 1, 2001; 159(3): 1259 - 1269.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Bass, O Riera-Lizarazu, E. Ananiev, S. Bordoli, H. Rines, R. Phillips, J. Sedat, D. Agard, and W. Cande
Evidence for the coincident initiation of homolog pairing and synapsis during the telomere-clustering (bouquet) stage of meiotic prophase
J. Cell Sci., January 3, 2000; 113(6): 1033 - 1042.
[Abstract] [PDF]

Home page
 JCBHome page
P. M. Carlton and W. Z. Cande
Telomeres act autonomously in maize to organize the meiotic bouquet from a semipolarized chromosome orientation
J. Cell Biol., April 15, 2002; 157(2): 231 - 242.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists