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Plant Cell, Vol. 11, 927-936, May 1999, Copyright © 1999, American Society of Plant Physiologists
Feedback Regulation of GA5 Expression and Metabolic Engineering of Gibberellin Levels in Arabidopsis
Yun-Ling Xua,
Li Lia,
Douglas A. Gageb, and
Jan A. D. Zeevaarta
a Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312
b Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824-1319
Correspondence to:
Jan A. D. Zeevaart, zeevaart{at}pilot.msu.edu (E-mail), 517-353-9168 (fax)
The gibberellin (GA) 20-oxidase encoded by the GA5 gene of Arabidopsis directs GA biosynthesis to active GAs, whereas that encoded by the P16 gene of pumpkin endosperm leads to biosynthesis of inactive GAs. Negative feedback regulation of GA5 expression was demonstrated in stems of Arabidopsis by bioactive GAs but not by inactive GA. In transgenic Arabidopsis plants overexpressing P16, there was a severe reduction in the amounts of C20–GA intermediates, accumulation of large amounts of inactive GA25 and GA17, a reduction in GA4 content, and a small increase in GA1. However, due to feedback regulation, expression of GA5 and GA4, the gene coding for the subsequent 3ß-hydroxylase, was greatly increased to compensate for the effects of the P16 transgene. Consequently, stem height was only slightly reduced in the transgenic plants.
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