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Plant Cell, Vol. 11, 1061-1072, June 1999, Copyright © 1999, American Society of Plant Physiologists
Silencing Gene Expression of the Ethylene-Forming Enzyme Results in a Reversible Inhibition of Ovule Development in Transgenic Tobacco Plants
Domenico De Martinisa and
Celestina Mariania
a Department of Experimental Botany, Graduate School Experimental Plant Sciences, Catholic University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
Correspondence to:
Celestina Mariani, mariani{at}sci.kun.nl (E-mail), 31-24-3652490 (fax)
To study the role of ethylene in plant reproduction, we constructed transgenic tobacco plants in which the expression of a pistil-specific gene coding for the ethylene-forming enzyme 1-aminocyclopropane-1-carboxylate oxidase was inhibited. Flowers from transgenic plants showed female sterility due to an arrest in ovule development. Megasporogenesis did not occur, and ovules did not reach maturity. When pollinated, pollen tubes were able to reach the ovary but did not penetrate into the immature ovule in transgenic plants. Flower treatment with an ethylene source resulted in a functional recovery of ovule development and restored guidance of the pollen tube tip into the ovule micropyle that resulted in seed set. The recovery was abolished if inhibitors of ethylene action were present. These results demonstrate that the plant hormone ethylene is required during the very early stages of female sporogenesis and ultimately to enable fertilization.
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