|
Plant Cell, Vol. 11, 1141-1152, June 1999, Copyright © 1999, American Society of Plant Physiologists
ATP Binding Cassette Modulators Control Abscisic Acid–Regulated Slow Anion Channels in Guard Cells
Nathalie Leonhardta,
Alain Vavasseura, and
Cyrille Forestiera
a Commissariat à l'Energie Atomique Cadarache, Direction des Sciences du Vivant, Département d'Ecophysiologie Végétale et Microbiologie, Laboratoire des Echanges Membranaires et Signalisation, BP1-F13108, St. Paul-lez-Durance, France
Correspondence to:
Cyrille Forestier, cforestier{at}cea.fr (E-mail), 33-4-42-25-46-56 (fax)
In animal cells, ATP binding cassette (ABC) proteins are a large family of transporters that includes the sulfonylurea receptor and the cystic fibrosis transmembrane conductance regulator (CFTR). These two ABC proteins possess an ion channel activity and bind specific sulfonylureas, such as glibenclamide, but homologs have not been identified in plant cells. We recently have shown that there is an ABC protein in guard cells that is involved in the control of stomatal movements and guard cell outward K+ current. Because the CFTR, a chloride channel, is sensitive to glibenclamide and able to interact with K+ channels, we investigated its presence in guard cells. Potent CFTR inhibitors, such as glibenclamide and diphenylamine-2-carboxylic acid, triggered stomatal opening in darkness. The guard cell protoplast slow anion current that was recorded using the whole-cell patch–clamp technique was inhibited rapidly by glibenclamide in a dose-dependent manner; the concentration producing half-maximum inhibition was at 3 µM. Potassium channel openers, which bind to and act through the sulfonylurea receptor in animal cells, completely suppressed the stomatal opening induced by glibenclamide and recovered the glibenclamide-inhibited slow anion current. Abscisic acid is known to regulate slow anion channels and in our study was able to relieve glibenclamide inhibition of slow anion current. Moreover, in epidermal strip bioassays, the stomatal closure triggered by Ca2+ or abscisic acid was reversed by glibenclamide. These results suggest that the slow anion channel is an ABC protein or is tightly controlled by such a protein that interacts with the abscisic acid signal transduction pathway in guard cells.
This article has been cited by other articles:
|
|
|
|
 
S.-J. Wu, Y.-S. Liu, and J.-Y. Wu
The Signaling Role of Extracellular ATP and its Dependence on Ca2+ Flux in Elicitation of Salvia miltiorrhiza Hairy Root Cultures
Plant Cell Physiol.,
April 1, 2008;
49(4):
617 - 624.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Suh, Y.-F. Wang, A. Frelet, N. Leonhardt, M. Klein, C. Forestier, B. Mueller-Roeber, M. H. Cho, E. Martinoia, and J. I. Schroeder
The ATP Binding Cassette Transporter AtMRP5 Modulates Anion and Calcium Channel Activities in Arabidopsis Guard Cells
J. Biol. Chem.,
January 19, 2007;
282(3):
1916 - 1924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Nilson and S. M. Assmann
The Control of Transpiration. Insights from Arabidopsis
Plant Physiology,
January 1, 2007;
143(1):
19 - 27.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Colcombet, F. Lelievre, S. Thomine, H. Barbier-Brygoo, and J.-M. Frachisse
Distinct pH regulation of slow and rapid anion channels at the plasma membrane of Arabidopsis thaliana hypocotyl cells
J. Exp. Bot.,
July 1, 2005;
56(417):
1897 - 1903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Brault, Z. Amiar, A.-M. Pennarun, M. Monestiez, Z. Zhang, D. Cornel, O. Dellis, H. Knight, F. Bouteau, and J.-P. Rona
Plasma Membrane Depolarization Induced by Abscisic Acid in Arabidopsis Suspension Cells Involves Reduction of Proton Pumping in Addition to Anion Channel Activation, Which Are Both Ca2+ Dependent
Plant Physiology,
May 1, 2004;
135(1):
231 - 243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Cherel
Regulation of K+ channel activities in plants: from physiological to molecular aspects
J. Exp. Bot.,
February 1, 2004;
55(396):
337 - 351.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. K. Lee, M. Kwon, J.-H. Ko, H. Yi, M. G. Hwang, S. Chang, and M. H. Cho
Binding of Sulfonylurea by AtMRP5, an Arabidopsis Multidrug Resistance-Related Protein That Functions in Salt Tolerance
Plant Physiology,
January 1, 2004;
134(1):
528 - 538.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Jasinski, E. Ducos, E. Martinoia, and M. Boutry
The ATP-Binding Cassette Transporters: Structure, Function, and Gene Family Comparison between Rice and Arabidopsis
Plant Physiology,
March 1, 2003;
131(3):
1169 - 1177.
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-Q. Guo, J. Young, and N. M. Crawford
The Nitrate Transporter AtNRT1.1 (CHL1) Functions in Stomatal Opening and Contributes to Drought Susceptibility in Arabidopsis
PLANT CELL,
January 1, 2003;
15(1):
107 - 117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Kwak, J.-H. Moon, Y. Murata, K. Kuchitsu, N. Leonhardt, A. DeLong, and J. I. Schroeder
Disruption of a Guard Cell-Expressed Protein Phosphatase 2A Regulatory Subunit, RCN1, Confers Abscisic Acid Insensitivity in Arabidopsis
PLANT CELL,
November 1, 2002;
14(11):
2849 - 2861.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J. Allen, Y. Murata, S. P. Chu, M. Nafisi, and J. I. Schroeder
Hypersensitivity of Abscisic Acid-Induced Cytosolic Calcium Increases in the Arabidopsis Farnesyltransferase Mutant era1-2
PLANT CELL,
July 1, 2002;
14(7):
1649 - 1662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Eckardt
Move It on Out with MATEs
PLANT CELL,
July 1, 2001;
13(7):
1477 - 1480.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-H. Zhang, P. R. Ryan, and S. D. Tyerman
Malate-Permeable Channels and Cation Channels Activated by Aluminum in the Apical Cells of Wheat Roots
Plant Physiology,
March 1, 2001;
125(3):
1459 - 1472.
[Abstract]
[Full Text]
|
|
|
|
|
