Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (96)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dai, N.
Right arrow Articles by Granot, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dai, N.
Right arrow Articles by Granot, D.
Agricola
Right arrow Articles by Dai, N.
Right arrow Articles by Granot, D.
Plant Cell, Vol. 11, 1253-1266, July 1999, Copyright © 1999, American Society of Plant Physiologists

Overexpression of Arabidopsis Hexokinase in Tomato Plants Inhibits Growth, Reduces Photosynthesis, and Induces Rapid Senescence

Nir Daia, Arthur Schaffera, Marina Petreikova, Yosepha Shahakb, Yuri Gillerb, Kira Ratnerb, Alex Levinec, and David Granota
a Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
b Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
c Department of Plant Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel

Correspondence to: David Granot, granot{at}agri.huji.ac.il (E-mail), 972-3-9669642 (fax)

Sugars are key regulatory molecules that affect diverse processes in higher plants. Hexokinase is the first enzyme in hexose metabolism and may be a sugar sensor that mediates sugar regulation. We present evidence that hexokinase is involved in sensing endogenous levels of sugars in photosynthetic tissues and that it participates in the regulation of senescence, photosynthesis, and growth in seedlings as well as in mature plants. Transgenic tomato plants overexpressing the Arabidopsis hexokinase-encoding gene AtHXK1 were produced. Independent transgenic plants carrying single copies of AtHXK1 were characterized by growth inhibition, the degree of which was found to correlate directly to the expression and activity of AtHXK1. Reciprocal grafting experiments suggested that the inhibitory effect occurred when AtHXK1 was expressed in photosynthetic tissues. Accordingly, plants with increased AtHXK1 activity had reduced chlorophyll content in their leaves, reduced photosynthesis rates, and reduced photochemical quantum efficiency of photosystem II reaction centers compared with plants without increased AtHXK1 activity. In addition, the transgenic plants underwent rapid senescence, suggesting that hexokinase is also involved in senescence regulation. Fruit weight, starch content in young fruits, and total soluble solids in mature fruits were also reduced in the transgenic plants. The results indicate that endogenous hexokinase activity is not rate limiting for growth; rather, they support the role of hexokinase as a regulatory enzyme in photosynthetic tissues, in which it regulates photosynthesis, growth, and senescence.




This article has been cited by other articles:


Home page
 J Exp BotHome page
W. G. van Doorn
Is the onset of senescence in leaf cells of intact plants due to low or high sugar levels?
J. Exp. Bot., May 2, 2008; (2008) ern076v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Aki, M. Konishi, T. Kikuchi, T. Fujimori, T. Yoneyama, and S. Yanagisawa
Distinct modulations of the hexokinase1-mediated glucose response and hexokinase1-independent processes by HYS1/CPR5 in Arabidopsis
J. Exp. Bot., September 6, 2007; (2007) erm169v2.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Tholen, T. L. Pons, L. A.C.J. Voesenek, and H. Poorter
Ethylene Insensitivity Results in Down-Regulation of Rubisco Expression and Photosynthetic Capacity in Tobacco
Plant Physiology, July 1, 2007; 144(3): 1305 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Calenge, V. Saliba-Colombani, S. Mahieu, O. Loudet, F. Daniel-Vedele, and A. Krapp
Natural Variation for Carbohydrate Content in Arabidopsis. Interaction with Complex Traits Dissected by Quantitative Genetics
Plant Physiology, August 1, 2006; 141(4): 1630 - 1643.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. V. Davies, L. V. T. Shepherd, M. M. Burrell, F. Carrari, E. Urbanczyk-Wochniak, A. Leisse, R. D. Hancock, M. Taylor, R. Viola, H. Ross, et al.
Modulation of Fructokinase Activity of Potato (Solanum tuberosum) Results in Substantial Shifts in Tuber Metabolism
Plant Cell Physiol., July 1, 2005; 46(7): 1103 - 1115.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. He, M. Osaki, M. Takebe, T. Shinano, and J. Wasaki
Endogenous hormones and expression of senescence-related genes in different senescent types of maize
J. Exp. Bot., April 1, 2005; 56(414): 1117 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
I. TERASHIMA, T. ARAYA, S.-I. MIYAZAWA, K. SONE, and S. YANO
Construction and Maintenance of the Optimal Photosynthetic Systems of the Leaf, Herbaceous Plant and Tree: an Eco-developmental Treatise
Ann. Bot., February 1, 2005; 95(3): 507 - 519.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Soudry, S. Ulitzur, and S. Gepstein
Accumulation and remobilization of amino acids during senescence of detached and attached leaves: in planta analysis of tryptophan levels by recombinant luminescent bacteria
J. Exp. Bot., February 1, 2005; 56(412): 695 - 702.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. E. Balibrea Lara, M.-C. Gonzalez Garcia, T. Fatima, R. Ehness, T. K. Lee, R. Proels, W. Tanner, and T. Roitsch
Extracellular Invertase Is an Essential Component of Cytokinin-Mediated Delay of Senescence
PLANT CELL, May 1, 2004; 16(5): 1276 - 1287.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. N. Harrington and D. R. Bush
The Bifunctional Role of Hexokinase in Metabolism and Glucose Signaling
PLANT CELL, November 1, 2003; 15(11): 2493 - 2496.
[Full Text] [PDF]

Home page
 Plant CellHome page
P. Giege, J. L. Heazlewood, U. Roessner-Tunali, A. H. Millar, A. R. Fernie, C. J. Leaver, and L. J. Sweetlove
Enzymes of Glycolysis Are Functionally Associated with the Mitochondrion in Arabidopsis Cells
PLANT CELL, September 1, 2003; 15(9): 2140 - 2151.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
U. Roessner-Tunali, B. Hegemann, A. Lytovchenko, F. Carrari, C. Bruedigam, D. Granot, and A. R. Fernie
Metabolic Profiling of Transgenic Tomato Plants Overexpressing Hexokinase Reveals That the Influence of Hexose Phosphorylation Diminishes during Fruit Development
Plant Physiology, September 1, 2003; 133(1): 84 - 99.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. J. Paul and T. K. Pellny
Carbon metabolite feedback regulation of leaf photosynthesis and development
J. Exp. Bot., January 3, 2003; 54(382): 539 - 547.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
N. V. Fedoroff
Cross-Talk in Abscisic Acid Signaling
Sci. Signal., July 9, 2002; 2002(140): re10 - re10.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Odanaka, A. B. Bennett, and Y. Kanayama
Distinct Physiological Roles of Fructokinase Isozymes Revealed by Gene-Specific Suppression of Frk1 and Frk2 Expression in Tomato
Plant Physiology, July 1, 2002; 129(3): 1119 - 1126.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. J. Paul and C. H. Foyer
Sink regulation of photosynthesis
J. Exp. Bot., July 1, 2001; 52(360): 1383 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. S. da-Silva, G. L. Rezende, and A. Galina
Subcellular distribution and kinetic properties of cytosolic and non-cytosolic hexokinases in maize seedling roots: implications for hexose phosphorylation
J. Exp. Bot., June 1, 2001; 52(359): 1191 - 1201.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. He, W. Tang, J. D. Swain, A. L. Green, T. P. Jack, and S. Gan
Networking Senescence-Regulating Pathways by Using Arabidopsis Enhancer Trap Lines
Plant Physiology, June 1, 2001; 126(2): 707 - 716.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Wingler, T. Fritzius, A. Wiemken, T. Boller, and R. A. Aeschbacher
Trehalose Induces the ADP-Glucose Pyrophosphorylase Gene, ApL3, and Starch Synthesis in Arabidopsis
Plant Physiology, September 1, 2000; 124(1): 105 - 114.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
F. Arenas-Huertero, A. Arroyo, L. Zhou, J. Sheen, and P. León
Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar
Genes & Dev., August 15, 2000; 14(16): 2085 - 2096.
[Abstract] [Full Text]

Home page
 Plant CellHome page
Y. He and S. Gan
A Gene Encoding an Acyl Hydrolase Is Involved in Leaf Senescence in Arabidopsis
PLANT CELL, April 1, 2002; 14(4): 805 - 815.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists