Plant Cell email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ward, B. M.
Right arrow Articles by Lazarowitz, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ward, B. M.
Right arrow Articles by Lazarowitz, S. G.
Agricola
Right arrow Articles by Ward, B. M.
Right arrow Articles by Lazarowitz, S. G.
Plant Cell, Vol. 11, 1267-1276, July 1999, Copyright © 1999, American Society of Plant Physiologists

Nuclear Export in Plants: Use of Geminivirus Movement Proteins for a Cell-Based Export Assay

Brian M. Warda and Sondra G. Lazarowitza
a Department of Microbiology, University of Illinois, Urbana, Illinois 61801

Correspondence to: Sondra G. Lazarowitz, at Department of Plant Pathology, Cornell University, Ithaca, NY 14853., SGL5{at}cornell.edu (E-mail), 607-255-4471 (fax)

The nuclear export of proteins and RNAs has been studied in heterokaryons or by microinjecting test substrates into nuclei of HeLa cells or Xenopus oocytes. We have previously shown that the two movement proteins BR1 and BL1 encoded by the plant pathogenic squash leaf curl virus act in a coordinated manner to facilitate virus cell-to-cell movement and that one of these (BR1) is a nuclear shuttle protein. By using a novel in vivo cell-based assay for nuclear export in which nuclear-localized BR1 is trapped by BL1 and redirected to the cortical cytoplasm, we demonstrate that residues 177 to 198 of BR1 contain a leucine-rich nuclear export signal (NES) of the type found in the Rev protein encoded by the human immunodeficiency virus and in Xenopus TFIIIA. We further show that the TFIIIA NES can functionally replace the NES of BR1 in both nuclear export and viral infectivity. These findings suggest that this basic pathway for nuclear export is highly conserved among plant and animal cells and in yeast.




This article has been cited by other articles:


Home page
 J Exp BotHome page
I. Meier
Composition of the plant nuclear envelope: theme and variations
J. Exp. Bot., January 1, 2007; 58(1): 27 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. Tillemans, I. Leponce, G. Rausin, L. Dispa, and P. Motte
Insights into Nuclear Organization in Plants as Revealed by the Dynamic Distribution of Arabidopsis SR Splicing Factors
PLANT CELL, November 1, 2006; 18(11): 3218 - 3234.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. F. Carvalho, R. Turgeon, and S. G. Lazarowitz
The Geminivirus Nuclear Shuttle Protein NSP Inhibits the Activity of AtNSI, a Vascular-Expressed Arabidopsis Acetyltransferase Regulated with the Sink-to-Source Transition
Plant Physiology, April 1, 2006; 140(4): 1317 - 1330.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
O. Guerra-Peraza, D. Kirk, V. Seltzer, K. Veluthambi, A. C. Schmit, T. Hohn, and E. Herzog
Coat proteins of Rice tungro bacilliform virus and Mungbean yellow mosaic virus contain multiple nuclear-localization signals and interact with importin {alpha}
J. Gen. Virol., June 1, 2005; 86(6): 1815 - 1826.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Hussain, S. Mansoor, S. Iram, A. N. Fatima, and Y. Zafar
The Nuclear Shuttle Protein of Tomato Leaf Curl New Delhi Virus Is a Pathogenicity Determinant
J. Virol., April 1, 2005; 79(7): 4434 - 4439.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Haas, A. Geldreich, M. Bureau, L. Dupuis, V. Leh, G. Vetter, K. Kobayashi, T. Hohn, L. Ryabova, P. Yot, et al.
The Open Reading Frame VI Product of Cauliflower mosaic virus Is a Nucleocytoplasmic Protein: Its N Terminus Mediates Its Nuclear Export and Formation of Electron-Dense Viroplasms
PLANT CELL, March 1, 2005; 17(3): 927 - 943.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. F. Carvalho and S. G. Lazarowitz
Interaction of the Movement Protein NSP and the Arabidopsis Acetyltransferase AtNSI Is Necessary for Cabbage Leaf Curl Geminivirus Infection and Pathogenicity
J. Virol., October 15, 2004; 78(20): 11161 - 11171.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Hehnle, C. Wege, and H. Jeske
Interaction of DNA with the Movement Proteins of Geminiviruses Revisited
J. Virol., July 15, 2004; 78(14): 7698 - 7706.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
E. V. Ryabov, S. H. Kim, and M. Taliansky
Identification of a nuclear localization signal and nuclear export signal of the umbraviral long-distance RNA movement protein
J. Gen. Virol., May 1, 2004; 85(5): 1329 - 1333.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. C. McGarry, Y. D. Barron, M. F. Carvalho, J. E. Hill, D. Gold, E. Cheung, W. L. Kraus, and S. G. Lazarowitz
A Novel Arabidopsis Acetyltransferase Interacts with the Geminivirus Movement Protein NSP
PLANT CELL, July 1, 2003; 15(7): 1605 - 1618.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. M. Bollman, M. J. Aukerman, M.-Y. Park, C. Hunter, T. Z. Berardini, and R. S. Poethig
HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis
Development, April 15, 2003; 130(8): 1493 - 1504.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
Y. Zhao, R. A. Owens, and R. W. Hammond
Use of a vector based on Potato virus X in a whole plant assay to demonstrate nuclear targeting of Potato spindle tuber viroid
J. Gen. Virol., June 1, 2001; 82(6): 1491 - 1497.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
D. Heerklotz, P. Döring, F. Bonzelius, S. Winkelhaus, and L. Nover
The Balance of Nuclear Import and Export Determines the Intracellular Distribution and Function of Tomato Heat Stress Transcription Factor HsfA2
Mol. Cell. Biol., March 1, 2001; 21(5): 1759 - 1768.
[Abstract] [Full Text]

Home page
 Plant CellHome page
M. R. Morra and I. T. D. Petty
Tissue Specificity of Geminivirus Infection Is Genetically Determined
PLANT CELL, November 1, 2000; 12(11): 2259 - 2270.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists