|
Plant Cell, Vol. 11, 1319-1336, July 1999, Copyright © 1999, American Society of Plant Physiologists
Epigenetic Interactions among Three dTph1 Transposons in Two Homologous Chromosomes Activate a New Excision–Repair Mechanism in Petunia
Adèle van Houwelingena,
Erik Souera,
Jos Mola, and
Ronald Koesa
a Department of Genetics, Institute for Molecular Biological Sciences, Vrije Universiteit, BioCentrum Amsterdam, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands
Correspondence to:
Ronald Koes, koes{at}bio.vu.nl (E-mail), 31-20-4447155 (fax)
Unstable anthocyanin3 (an3) alleles of petunia with insertions of the Activator/Dissociation–like transposon dTph1 fall into two classes that differ in their genetic behavior. Excision of the (single) dTph1 insertion from class 1 an3 alleles results in the formation of a footprint, similar to the "classical" mechanism observed for excisions of maize and snapdragon transposons. By contrast, dTph1 excision and gap repair in class 2 an3 alleles occurs via a newly discovered mechanism that does not generate a footprint at the empty donor site. This novel mechanism depends on the presence of two additional dTph1 elements: one located in cis, 30 bp upstream of the an3 translation start in the same an3 allele, and a homologous copy, which is located in trans in the homologous an3 allele. Absence of the latter dTph1 element causes a heritable suppression of dTph1 excision–repair from the homologous an3 allele by the novel mechanism, which to some extent resembles paramutation. Thus, an epigenetic interaction among three dTph1 copies activates a novel recombination mechanism that eliminates a transposon insertion.
This article has been cited by other articles:
|
|
|
|
 
S. Kawasaki and E. Nitasaka
Characterization of Tpn1 Family in the Japanese Morning Glory: En/Spm-related Transposable Elements Capturing Host Genes
Plant Cell Physiol.,
July 15, 2004;
45(7):
933 - 944.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Stam, C. Belele, W. Ramakrishna, J. E. Dorweiler, J. L. Bennetzen, and V. L. Chandler
The Regulatory Regions Required for B' Paramutation and Expression Are Located Far Upstream of the Maize b1 Transcribed Sequences
Genetics,
October 1, 2002;
162(2):
917 - 930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Stam, C. Belele, J. E. Dorweiler, and V. L. Chandler
Differential chromatin structure within a tandem array 100 kb upstream of the maize b1 locus is associated with paramutation
Genes & Dev.,
August 1, 2002;
16(15):
1906 - 1918.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tobena-Santamaria, M. Bliek, K. Ljung, G. Sandberg, J. N.M. Mol, E. Souer, and R. Koes
FLOOZY of petunia is a flavin mono-oxygenase-like protein required for the specification of leaf and flower architecture
Genes & Dev.,
March 15, 2002;
16(6):
753 - 763.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Dubrana and L. Amar
Control of DNA excision efficiency in Paramecium
Nucleic Acids Res.,
November 15, 2001;
29(22):
4654 - 4662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Quattrocchio, J. Wing, K. van der Woude, E. Souer, N. de Vetten, J. Mol, and R. Koes
Molecular Analysis of the anthocyanin2 Gene of Petunia and Its Role in the Evolution of Flower Color
PLANT CELL,
August 1, 1999;
11(8):
1433 - 1444.
[Abstract]
[Full Text]
|
|
|
|
|
