Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (54)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, N.-C.
Right arrow Articles by Tsay, Y.-F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, N.-C.
Right arrow Articles by Tsay, Y.-F.
Agricola
Right arrow Articles by Huang, N.-C.
Right arrow Articles by Tsay, Y.-F.
Plant Cell, Vol. 11, 1381-1392, August 1999, Copyright © 1999, American Society of Plant Physiologists

Cloning and Functional Characterization of an Arabidopsis Nitrate Transporter Gene That Encodes a Constitutive Component of Low-Affinity Uptake

Nien-Chen Huanga, Kun-Hsiang Liua,b, Hau-Jan Loa, and Yi-Fang Tsaya
a Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
b Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan

Correspondence to: Yi-Fang Tsay, mbyftsay{at}ccvax.sinica.edu.tw (E-mail), 886-2-2782-6085 (fax)

The Arabidopsis CHL1 (AtNRT1) gene encodes an inducible component of low-affinity nitrate uptake, which necessitates a "two-component" model to account for the constitutive low-affinity uptake observed in physiological studies. Here, we report the cloning and characterization of a CHL1 homolog, AtNRT1:2 (originally named NTL1), with data to indicate that this gene encodes a constitutive component of low-affinity nitrate uptake. Transgenic plants expressing antisense AtNRT1:2 exhibited reduced nitrate-induced membrane depolarization and nitrate uptake activities in assays with 10 mM nitrate. Furthermore, transgenic plants expressing antisense AtNRT1:2 in the chl1-5 background exhibited an enhanced resistance to chlorate (7 mM as opposed to 2 mM for the chl1-5 mutant). Kinetic analysis of AtNRT1:2-injected Xenopus oocytes yielded a Km for nitrate of ~5.9 mM. In contrast to CHL1, AtNRT1:2 was constitutively expressed before and after nitrate exposure (it was repressed transiently only when the level of CHL1 mRNA started to increase significantly), and its mRNA was found primarily in root hairs and the epidermis in both young (root tips) and mature regions of roots. We conclude that low-affinity systems of nitrate uptake, like high-affinity systems, are composed of inducible and constitutive components and that with their distinct functions, they are part of an elaborate nitrate uptake network in Arabidopsis.




This article has been cited by other articles:


Home page
 Plant CellHome page
S.-C. Fan, C.-S. Lin, P.-K. Hsu, S.-H. Lin, and Y.-F. Tsay
The Arabidopsis Nitrate Transporter NRT1.7, Expressed in Phloem, Is Responsible for Source-to-Sink Remobilization of Nitrate
PLANT CELL, September 1, 2009; 21(9): 2750 - 2761.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Almagro, S. H. Lin, and Y. F. Tsay
Characterization of the Arabidopsis Nitrate Transporter NRT1.6 Reveals a Role of Nitrate in Early Embryo Development
PLANT CELL, December 1, 2008; 20(12): 3289 - 3299.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S.-H. Lin, H.-F. Kuo, G. Canivenc, C.-S. Lin, M. Lepetit, P.-K. Hsu, P. Tillard, H.-L. Lin, Y.-Y. Wang, C.-B. Tsai, et al.
Mutation of the Arabidopsis NRT1.5 Nitrate Transporter Causes Defective Root-to-Shoot Nitrate Transport
PLANT CELL, September 1, 2008; 20(9): 2514 - 2528.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Segonzac, J.-C. Boyer, E. Ipotesi, W. Szponarski, P. Tillard, B. Touraine, N. Sommerer, M. Rossignol, and R. Gibrat
Nitrate Efflux at the Root Plasma Membrane: Identification of an Arabidopsis Excretion Transporter
PLANT CELL, November 1, 2007; 19(11): 3760 - 3777.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. J. Miller, X. Fan, M. Orsel, S. J. Smith, and D. M. Wells
Nitrate transport and signalling
J. Exp. Bot., July 1, 2007; 58(9): 2297 - 2306.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Remans, P. Nacry, M. Pervent, S. Filleur, E. Diatloff, E. Mounier, P. Tillard, B. G. Forde, and A. Gojon
The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches
PNAS, December 12, 2006; 103(50): 19206 - 19211.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Krouk, P. Tillard, and A. Gojon
Regulation of the High-Affinity NO3- Uptake System by NRT1.1-Mediated NO3- Demand Signaling in Arabidopsis
Plant Physiology, November 1, 2006; 142(3): 1075 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
W. M. WATERWORTH and C. M. BRAY
Enigma Variations for Peptides and Their Transporters in Higher Plants
Ann. Bot., July 1, 2006; 98(1): 1 - 8.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
C.-C. Chiu, C.-S. Lin, A.-P. Hsia, R.-C. Su, H.-L. Lin, and Y.-F. Tsay
Mutation of a Nitrate Transporter, AtNRT1:4, Results in a Reduced Petiole Nitrate Content and Altered Leaf Development
Plant Cell Physiol., September 15, 2004; 45(9): 1139 - 1148.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Munos, C. Cazettes, C. Fizames, F. Gaymard, P. Tillard, M. Lepetit, L. Lejay, and A. Gojon
Transcript Profiling in the chl1-5 Mutant of Arabidopsis Reveals a Role of the Nitrate Transporter NRT1.1 in the Regulation of Another Nitrate Transporter, NRT2.1
PLANT CELL, September 1, 2004; 16(9): 2433 - 2447.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-S. Chiang, G. Stacey, and Y.-F. Tsay
Mechanisms and Functional Properties of Two Peptide Transporters, AtPTR2 and fPTR2
J. Biol. Chem., July 16, 2004; 279(29): 30150 - 30157.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Malagoli, P. Laine, E. Le Deunff, L. Rossato, B. Ney, and A. Ourry
Modeling Nitrogen Uptake in Oilseed Rape cv Capitol during a Growth Cycle Using Influx Kinetics of Root Nitrate Transport Systems and Field Experimental Data
Plant Physiology, January 1, 2004; 134(1): 388 - 400.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Santi, G. Locci, R. Monte, R. Pinton, and Z. Varanini
Induction of nitrate uptake in maize roots: expression of a putative high-affinity nitrate transporter and plasma membrane H+-ATPase isoforms
J. Exp. Bot., August 1, 2003; 54(389): 1851 - 1864.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Loque, P. Tillard, A. Gojon, and M. Lepetit
Gene Expression of the NO3- Transporter NRT1.1 and the Nitrate Reductase NIA1 Is Repressed in Arabidopsis Roots by NO2-, the Product of NO3- Reduction
Plant Physiology, June 1, 2003; 132(2): 958 - 967.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Okamoto, J. J. Vidmar, and A. D. M. Glass
Regulation of NRT1 and NRT2 Gene Families of Arabidopsis thaliana: Responses to Nitrate Provision
Plant Cell Physiol., March 15, 2003; 44(3): 304 - 317.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Faure-Rabasse, E. Le Deunff, P. Laine, J. H. Macduff, and A. Ourry
Effects of nitrate pulses on BnNRT1 and BnNRT2 genes: mRNA levels and nitrate influx rates in relation to the duration of N deprivation in Brassica napus L.
J. Exp. Bot., August 1, 2002; 53(375): 1711 - 1721.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F.-Q. Guo, R. Wang, and N. M. Crawford
The Arabidopsis dual-affinity nitrate transporter gene AtNRT1.1 (CHL1) is regulated by auxin in both shoots and roots
J. Exp. Bot., April 15, 2002; 53(370): 835 - 844.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Cerezo, P. Tillard, S. Filleur, S. Munos, F. Daniel-Vedele, and A. Gojon
Major Alterations of the Regulation of Root NO3{-} Uptake Are Associated with the Mutation of Nrt2.1 and Nrt2.2 Genes in Arabidopsis
Plant Physiology, September 1, 2001; 127(1): 262 - 271.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F.-Q. Guo, R. Wang, M. Chen, and N. M. Crawford
The Arabidopsis Dual-Affinity Nitrate Transporter Gene AtNRT1.1 (CHL1) Is Activated and Functions in Nascent Organ Development during Vegetative and Reproductive Growth
PLANT CELL, August 1, 2001; 13(8): 1761 - 1777.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C.-M. Lin, S. Koh, G. Stacey, S.-M. Yu, T.-Y. Lin, and Y.-F. Tsay
Cloning and Functional Characterization of a Constitutively Expressed Nitrate Transporter Gene, OsNRT1, from Rice
Plant Physiology, February 1, 2000; 122(2): 379 - 388.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C.-F. Chuang and E. M. Meyerowitz
Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana
PNAS, April 25, 2000; 97(9): 4985 - 4990.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists