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Plant Cell, Vol. 11, 1445-1456, August 1999, Copyright © 1999, American Society of Plant Physiologists
Light QualityDependent Nuclear Import of the Plant Photoreceptors Phytochrome A and B
Stefan Kirchera,
Laszlo Kozma-Bognarb,
Lana Kima,
Eva Adamb,
Klaus Hartera,
Eberhard Schäfera, and
Ferenc Nagyb
a Institut für Biologie II/Botanik, Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany
b Institute of Plant Biology, Biological Research Center, P.O. Box 521, H-6701 Szeged, Hungary
Correspondence to:
Ferenc Nagy, nagyf{at}nucleus.szbk.u-szeged.hu (E-mail), 36-62-433434 (fax)
The phytochrome (phy) family of plant photoreceptors controls various aspects of photomorphogenesis. Overexpression of rice phyAgreen fluorescent protein (GFP) and tobacco phyBGFP fusion proteins in tobacco results in functional photoreceptors. phyAGFP and phyBGFP are localized in the cytosol of dark-adapted plants. In our experiments, red light treatment led to nuclear translocation of phyAGFP and phyBGFP, albeit with different kinetics. Red lightinduced nuclear import of phyBGFP, but not that of phyAGFP, was inhibited by far-red light. Far-red light alone only induced nuclear translocation of phyAGFP. These observations indicate that nuclear import of phyAGFP is controlled by a very low fluence response, whereas translocation of phyBGFP is regulated by a low fluence response of phytochrome. Thus, light-regulated nucleocytoplasmic partitioning of phyA and phyB is a major step in phytochrome signaling.
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