Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (72)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hinz, G.
Right arrow Articles by Hohl, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hinz, G.
Right arrow Articles by Hohl, I.
Agricola
Right arrow Articles by Hinz, G.
Right arrow Articles by Hohl, I.
Plant Cell, Vol. 11, 1509-1524, August 1999, Copyright © 1999, American Society of Plant Physiologists

Vacuolar Storage Proteins and the Putative Vacuolar Sorting Receptor BP-80 Exit the Golgi Apparatus of Developing Pea Cotyledons in Different Transport Vesicles

Giselbert Hinza, Stefan Hillmera, Matthias Bäumera, and Inge Hohla
a Abteilung Strukturelle Zellphysiologie, Albrecht-von-Haller Institut für Pflanzenwissenschaften, Universität Göttingen, Untere Karspüle, D-37073 Göttingen, Germany

Correspondence to: Giselbert Hinz, ghinz{at}gwdg.de (E-mail), 49-551-397823 (fax)

In the parenchyma cells of developing legume cotyledons, storage proteins are deposited in a special type of vacuole, known as the protein storage vacuole (PSV). Storage proteins are synthesized at the endoplasmic reticulum and pass through the Golgi apparatus. In contrast to lysosomal acid hydrolases, storage proteins exit the Golgi apparatus in 130-nm-diameter electron-dense vesicles rather than in clathrin-coated vesicles. By combining isopycnic and rate zonal sucrose density gradient centrifugation with phase partitioning, we obtained a highly enriched dense vesicle fraction. This fraction contained prolegumin, which is the precursor of one of the major storage proteins. In dense vesicles, prolegumin occurred in a more aggregated form than it did in the endoplasmic reticulum. The putative vacuolar sorting receptor BP-80 was highly enriched in purified clathrin-coated vesicles, which, in turn, did not contain prolegumin. The amount of BP-80 was markedly reduced in the dense vesicle fraction. This result was confirmed by quantitative immunogold labeling of cryosections of pea cotyledons: whereas antibodies raised against BP-80 significantly labeled the Golgi stacks, labeling of the dense vesicles could not be detected. In contrast, 90% of the dense vesicles were labeled with antibodies raised against {alpha}-TIP (for tonoplast intrinsic protein), which is the aquaporin specific for the membrane of the PSV. These results lead to the conclusions that storage proteins and {alpha}-TIP are delivered via the same vesicular pathway into the PSVs and that the dense vesicles that carry these proteins in turn do not contain BP-80.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
I. Hwang
Sorting and Anterograde Trafficking at the Golgi Apparatus
Plant Physiology, October 1, 2008; 148(2): 673 - 683.
[Full Text] [PDF]

Home page
 J Exp BotHome page
A. von Lupke, G. Schauermann, I. Feussner, and G. Hinz
Peripheral membrane proteins mediate binding of vacuolar storage proteins to membranes of the secretory pathway of developing pea cotyledons
J. Exp. Bot., April 1, 2008; 59(6): 1327 - 1340.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. C. Rogers
Multiple Vacuoles in Plant Cells
Plant Physiology, March 1, 2008; 146(3): 1024 - 1025.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Olbrich, S. Hillmer, G. Hinz, P. Oliviusson, and D. G. Robinson
Newly Formed Vacuoles in Root Meristems of Barley and Pea Seedlings Have Characteristics of Both Protein Storage and Lytic Vacuoles
Plant Physiology, December 1, 2007; 145(4): 1383 - 1394.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. J. Sohn, M. Rojas-Pierce, S. Pan, C. Carter, A. Serrano-Mislata, F. Madueno, E. Rojo, M. Surpin, and N. V. Raikhel
The shoot meristem identity gene TFL1 is involved in flower development and trafficking to the protein storage vacuole
PNAS, November 20, 2007; 104(47): 18801 - 18806.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Muntz
Protein dynamics and proteolysis in plant vacuoles
J. Exp. Bot., July 1, 2007; 58(10): 2391 - 2407.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Sanmartin, A. Ordonez, E. J. Sohn, S. Robert, J. J. Sanchez-Serrano, M. A. Surpin, N. V. Raikhel, and E. Rojo
Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis
PNAS, February 27, 2007; 104(9): 3645 - 3650.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Fuji, T. Shimada, H. Takahashi, K. Tamura, Y. Koumoto, S. Utsumi, K. Nishizawa, N. Maruyama, and I. Hara-Nishimura
Arabidopsis Vacuolar Sorting Mutants (green fluorescent seed) Can Be Identified Efficiently by Secretion of Vacuole-Targeted Green Fluorescent Protein in Their Seeds
PLANT CELL, February 1, 2007; 19(2): 597 - 609.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. S. Otegui, R. Herder, J. Schulze, R. Jung, and L. A. Staehelin
The Proteolytic Processing of Seed Storage Proteins in Arabidopsis Embryo Cells Starts in the Multivesicular Bodies
PLANT CELL, October 1, 2006; 18(10): 2567 - 2581.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Oliviusson, O. Heinzerling, S. Hillmer, G. Hinz, Y. C. Tse, L. Jiang, and D. G. Robinson
Plant Retromer, Localized to the Prevacuolar Compartment and Microvesicles in Arabidopsis, May Interact with Vacuolar Sorting Receptors
PLANT CELL, May 1, 2006; 18(5): 1239 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Oufattole, J. H. Park, M. Poxleitner, L. Jiang, and J. C. Rogers
Selective Membrane Protein Internalization Accompanies Movement from the Endoplasmic Reticulum to the Protein Storage Vacuole Pathway in Arabidopsis
PLANT CELL, November 1, 2005; 17(11): 3066 - 3080.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Park, D. Lee, G.-J. Lee, and I. Hwang
AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole
J. Cell Biol., August 29, 2005; 170(5): 757 - 767.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, J. P. Taylor, J. L. Hadlington, S. L. Hanton, C. J. Snowden, S. J. Fox, O. Foresti, F. Brandizzi, and J. Denecke
Receptor Salvage from the Prevacuolar Compartment Is Essential for Efficient Vacuolar Protein Targeting
PLANT CELL, January 1, 2005; 17(1): 132 - 148.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. C. Tse, B. Mo, S. Hillmer, M. Zhao, S. W. Lo, D. G. Robinson, and L. Jiang
Identification of Multivesicular Bodies as Prevacuolar Compartments in Nicotiana tabacum BY-2 Cells
PLANT CELL, March 1, 2004; 16(3): 672 - 693.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Bolte, S. Brown, and B. Satiat-Jeunemaitre
The N-myristoylated Rab-GTPase m-Rabmc is involved in post-Golgi trafficking events to the lytic vacuole in plant cells
J. Cell Sci., February 22, 2004; 117(6): 943 - 954.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Park, S. J. Kim, A. Vitale, and I. Hwang
Identification of the Protein Storage Vacuole and Protein Targeting to the Vacuole in Leaf Cells of Three Plant Species
Plant Physiology, February 1, 2004; 134(2): 625 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Watanabe, T. Shimada, K. Tamura, R. Matsushima, Y. Koumoto, M. Nishimura, and I. Hara-Nishimura
An ER-Localized Form of PV72, a Seed-Specific Vacuolar Sorting Receptor, Interferes the Transport of an NPIR-Containing Proteinase in Arabidopsis Leaves
Plant Cell Physiol., January 15, 2004; 45(1): 9 - 17.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Shimada, K. Fuji, K. Tamura, M. Kondo, M. Nishimura, and I. Hara-Nishimura
Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana
PNAS, December 23, 2003; 100(26): 16095 - 16100.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
U. NEUMANN, F. BRANDIZZI, and C. HAWES
Protein Transport in Plant Cells: In and Out of the Golgi{dagger}
Ann. Bot., August 1, 2003; 92(2): 167 - 180.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Pimpl, S. L. Hanton, J. P. Taylor, L. L. Pinto-daSilva, and J. Denecke
The GTPase ARF1p Controls the Sequence-Specific Vacuolar Sorting Route to the Lytic Vacuole
PLANT CELL, May 1, 2003; 15(5): 1242 - 1256.
[Abstract] [Full Text]

Home page
 J Exp BotHome page
V. Laval, F. Masclaux, A. Serin, M. Carriere, C. Roldan, M. Devic, R. F. Pont-Lezica, and J.-P. Galaud
Seed germination is blocked in Arabidopsis putative vacuolar sorting receptor (atbp80) antisense transformants
J. Exp. Bot., January 2, 2003; 54(381): 213 - 221.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Shimada, E. Watanabe, K. Tamura, Y. Hayashi, M. Nishimura, and I. Hara-Nishimura
A Vacuolar Sorting Receptor PV72 on the Membrane of Vesicles that Accumulate Precursors of Seed Storage Proteins (PAC Vesicles)
Plant Cell Physiol., October 15, 2002; 43(10): 1086 - 1095.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y.-B. Li, S. W. Rogers, Y. C. Tse, S. W. Lo, S. S. M. Sun, G.-Y. Jauh, and L. Jiang
BP-80 and Homologs are Concentrated on Post-Golgi, Probable Lytic Prevacuolar Compartments
Plant Cell Physiol., July 15, 2002; 43(7): 726 - 742.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Watanabe, T. Shimada, M. Kuroyanagi, M. Nishimura, and I. Hara-Nishimura
Calcium-mediated Association of a Putative Vacuolar Sorting Receptor PV72 with a Propeptide of 2S Albumin
J. Biol. Chem., March 1, 2002; 277(10): 8708 - 8715.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L. Jiang, T. E. Phillips, C. A. Hamm, Y. M. Drozdowicz, P. A. Rea, M. Maeshima, S. W. Rogers, and J. C. Rogers
The protein storage vacuole: a unique compound organelle
J. Cell Biol., December 10, 2001; 155(6): 991 - 1002.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Mitsuhashi, Y. Hayashi, Y. Koumoto, T. Shimada, T. Fukasawa-Akada, M. Nishimura, and I. Hara-Nishimura
A Novel Membrane Protein That Is Transported to Protein Storage Vacuoles via Precursor-Accumulating Vesicles
PLANT CELL, October 1, 2001; 13(10): 2361 - 2372.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Tormakangas, J. L. Hadlington, P. Pimpl, S. Hillmer, F. Brandizzi, T. H. Teeri, and J. Denecke
A Vacuolar Sorting Domain May Also Influence the Way in Which Proteins Leave the Endoplasmic Reticulum
PLANT CELL, September 1, 2001; 13(9): 2021 - 2032.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Frigerio, N. A. Jolliffe, A. Di Cola, D. H. Felipe, N. Paris, J.-M. Neuhaus, J. M. Lord, A. Ceriotti, and L. M. Roberts
The Internal Propeptide of the Ricin Precursor Carries a Sequence-Specific Determinant for Vacuolar Sorting
Plant Physiology, May 1, 2001; 126(1): 167 - 175.
[Abstract] [Full Text]

Home page
 JCBHome page
S. Hillmer, A. Movafeghi, D. G. Robinson, and G. Hinz
Vacuolar Storage Proteins Are Sorted in the cis-Cisternae of the Pea Cotyledon Golgi Apparatus
J. Cell Biol., January 2, 2001; 152(1): 41 - 50.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Pimpl, A. Movafeghi, S. Coughlan, J. Denecke, S. Hillmer, and D. G. Robinson
In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles
PLANT CELL, November 1, 2000; 12(11): 2219 - 2236.
[Abstract] [Full Text]

Home page
 Plant Cell PhysiolHome page
N. Mitsuhashi, T. Shimada, S. Mano, M. Nishimura, and I. Hara-Nishimura
Characterization of Organelles in the Vacuolar-Sorting Pathway by Visualization with GFP in Tobacco BY-2 Cells
Plant Cell Physiol., September 1, 2000; 41(9): 993 - 1001.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L. Jiang, T. E. Phillips, S. W. Rogers, and J. C. Rogers
Biogenesis of the Protein Storage Vacuole Crystalloid
J. Cell Biol., August 21, 2000; 150(4): 755 - 770.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
X. Cao, S. W. Rogers, J. Butler, L. Beevers, and J. C. Rogers
Structural Requirements for Ligand Binding by a Probable Plant Vacuolar Sorting Receptor
PLANT CELL, April 1, 2000; 12(4): 493 - 506.
[Abstract] [Full Text]

Home page
 Plant CellHome page
L. Jiang and J. C. Rogers
The Role of BP-80 and Homologs in Sorting Proteins to Vacuoles
PLANT CELL, November 1, 1999; 11(11): 2069 - 2071.
[Full Text]

Home page
 Plant CellHome page
G.-Y. Jauh, T. E. Phillips, and J. C. Rogers
Tonoplast Intrinsic Protein Isoforms as Markers for Vacuolar Functions
PLANT CELL, October 1, 1999; 11(10): 1867 - 1882.
[Abstract] [Full Text]

Home page
 Plant CellHome page
H. B. Smith
Vacuolar Protein Trafficking and Vesicles: Continuing to Sort It All Out
PLANT CELL, August 1, 1999; 11(8): 1377 - 1380.
[Full Text]

Home page
 Plant CellHome page
E. A. Miller, M. C. S. Lee, and M. A. Anderson
Identification and Characterization of a Prevacuolar Compartment in Stigmas of Nicotiana alata
PLANT CELL, August 1, 1999; 11(8): 1499 - 1508.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1999 by the American Society of Plant Biologists