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Plant Cell, Vol. 12, 1951-1960, October 2000, Copyright © 2000, American Society of Plant Physiologists
Insertion of OEP14 into the Outer Envelope Membrane Is Mediated by Proteinaceous Components of Chloroplasts
Shuh-Long Tua,b and
Hsou-min Lib
a Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 112, Taiwan
b Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan
Correspondence to:
Hsou-min Li, mbhmli{at}ccvax.sinica.edu.tw (E-mail), 011-886-2-2782-6085 (fax)
Most chloroplastic outer envelope membrane proteins are synthesized in the cytosol at their mature size without a cleavable targeting signal. Their insertion into the outer membrane is insensitive to thermolysin pretreatment of chloroplasts and does not require ATP. The insertion has been assumed to be mediated by a spontaneous mechanism or by interaction solely with the lipid components of the outer membrane. However, we show here that insertion of an outer membrane protein requires some trypsin-sensitive and some N-ethylmaleimide–sensitive components of chloroplasts. Association and insertion of the outer membrane protein are saturable and compete with the import of another outer membrane protein. These data suggest that import of chloroplastic outer membrane proteins occurs at specific proteinaceous sites on chloroplasts.
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