|
Plant Cell, Vol. 12, 2383-2394, December 2000, Copyright © 2000, American Society of Plant Physiologists
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
Justin O. Borevitza,
Yiji Xiaa,b,
Jack Blountb,
Richard A. Dixonb, and
Chris Lamba
a Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037
b Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
Correspondence to:
Chris Lamb, chris.lamb{at}bbsrc.ac.uk (E-mail), 44-1603-456844 (fax)
Plants produce a wide array of natural products, many of which are likely to be useful bioactive structures. Unfortunately, these complex natural products usually occur at very low abundance and with restricted tissue distribution, thereby hindering their evaluation. Here, we report a novel approach for enhancing the accumulation of natural products based on activation tagging by Agrobacterium-mediated transformation with a T-DNA that carries cauliflower mosaic virus 35S enhancer sequences at its right border. Among 5000 Arabidopsis activation-tagged lines, we found a plant that exhibited intense purple pigmentation in many vegetative organs throughout development. This upregulation of pigmentation reflected a dominant mutation that resulted in massive activation of phenylpropanoid biosynthetic genes and enhanced accumulation of lignin, hydroxycinnamic acid esters, and flavonoids, including various anthocyanins that were responsible for the purple color. These phenotypes, caused by insertion of the viral enhancer sequences adjacent to an MYB transcription factor gene, indicate that activation tagging can overcome the stringent genetic controls regulating the accumulation of specific natural products during plant development. Our findings suggest a functional genomics approach to the biotechnological evaluation of phytochemical biodiversity through the generation of massively enriched tissue sources for drug screening and for isolating underlying regulatory and biosynthetic genes.
This article has been cited by other articles:

|
 |

|
 |
 
T. Nakatsuka, K. S. Haruta, C. Pitaksutheepong, Y. Abe, Y. Kakizaki, K. Yamamoto, N. Shimada, S. Yamamura, and M. Nishihara
Identification and Characterization of R2R3-MYB and bHLH Transcription Factors Regulating Anthocyanin Biosynthesis in Gentian Flowers
Plant Cell Physiol.,
December 1, 2008;
49(12):
1818 - 1829.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Maes, D. Inze, and A. Goossens
Functional Specialization of the TRANSPARENT TESTA GLABRA1 Network Allows Differential Hormonal Control of Laminal and Marginal Trichome Initiation in Arabidopsis Rosette Leaves
Plant Physiology,
November 1, 2008;
148(3):
1453 - 1464.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Bashandy, L. Taconnat, J.-P. Renou, Y. Meyer, and J.-P. Reichheld
Accumulation of Flavonoids in an ntra ntrb Mutant Leads to Tolerance to UV-C
Mol Plant,
October 29, 2008;
(2008)
ssn065v1.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. A. E. Laitinen, M. Ainasoja, S. K. Broholm, T. H. Teeri, and P. Elomaa
Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida
J. Exp. Bot.,
October 1, 2008;
59(13):
3691 - 3703.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Bomal, F. Bedon, S. Caron, S. D. Mansfield, C. Levasseur, J. E. K. Cooke, S. Blais, L. Tremblay, M.-J. Morency, N. Pavy, et al.
Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis
J. Exp. Bot.,
October 1, 2008;
59(14):
3925 - 3939.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Peng, D. Hudson, A. Schofield, R. Tsao, R. Yang, H. Gu, Y.-M. Bi, and Steven. J. Rothstein
Adaptation of Arabidopsis to nitrogen limitation involves induction of anthocyanin synthesis which is controlled by the NLA gene
J. Exp. Bot.,
August 1, 2008;
59(11):
2933 - 2944.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Stout, E. Romero-Severson, M. O. Ruegger, and C. Chapple
Semidominant Mutations in Reduced Epidermal Fluorescence 4 Reduce Phenylpropanoid Content in Arabidopsis
Genetics,
April 1, 2008;
178(4):
2237 - 2251.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Yu, X. Chen, Y.-Y. Hong, Y. Wang, P. Xu, S.-D. Ke, H.-Y. Liu, J.-K. Zhu, D. J. Oliver, and C.-B. Xiang
Activated Expression of an Arabidopsis HD-START Protein Confers Drought Tolerance with Improved Root System and Reduced Stomatal Density
PLANT CELL,
April 1, 2008;
20(4):
1134 - 1151.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Raffaele, F. Vailleau, A. Leger, J. Joubes, O. Miersch, C. Huard, E. Blee, S. Mongrand, F. Domergue, and D. Roby
A MYB Transcription Factor Regulates Very-Long-Chain Fatty Acid Biosynthesis for Activation of the Hypersensitive Cell Death Response in Arabidopsis
PLANT CELL,
March 1, 2008;
20(3):
752 - 767.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Cai and C. C. Lashbrook
Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis ZINC FINGER PROTEIN2
Plant Physiology,
March 1, 2008;
146(3):
1305 - 1321.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Ma, M. Pooler, and R. Griesbach
Ratio of Myc and Myb Transcription Factors Regulates Anthocyanin Production in Orchid Flowers
J. Amer. Soc. Hort. Sci.,
January 1, 2008;
133(1):
133 - 138.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Qu, A. Desai, R. Wing, and V. Sundaresan
A Versatile Transposon-Based Activation Tag Vector System for Functional Genomics in Cereals and Other Monocot Plants
Plant Physiology,
January 1, 2008;
146(1):
189 - 199.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. Poustka, N. G. Irani, A. Feller, Y. Lu, L. Pourcel, K. Frame, and E. Grotewold
A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions
Plant Physiology,
December 1, 2007;
145(4):
1323 - 1335.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Jiang, X. Gao, L. Liao, N. P. Harberd, and X. Fu
Phosphate Starvation Root Architecture and Anthocyanin Accumulation Responses Are Modulated by the Gibberellin-DELLA Signaling Pathway in Arabidopsis
Plant Physiology,
December 1, 2007;
145(4):
1460 - 1470.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. J. Lightbourn, J. R. Stommel, and R. J. Griesbach
Epistatic Interactions Influencing Anthocyanin Gene Expression in Capsicum annuum
J. Amer. Soc. Hort. Sci.,
November 1, 2007;
132(6):
824 - 829.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Liu and M. C. Mehdy
A Nonclassical Arabinogalactan Protein Gene Highly Expressed in Vascular Tissues, AGP31, Is Transcriptionally Repressed by Methyl Jasmonic Acid in Arabidopsis
Plant Physiology,
November 1, 2007;
145(3):
863 - 874.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Morohashi, M. Zhao, M. Yang, B. Read, A. Lloyd, R. Lamb, and E. Grotewold
Participation of the Arabidopsis bHLH Factor GL3 in Trichome Initiation Regulatory Events
Plant Physiology,
November 1, 2007;
145(3):
736 - 746.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Alves-Ferreira, F. Wellmer, A. Banhara, V. Kumar, J. L. Riechmann, and E. M. Meyerowitz
Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development
Plant Physiology,
November 1, 2007;
145(3):
747 - 762.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. J. Griesbach, R. M. Beck, J. Hammond, and J. R. Stommel
Gene Expression in the Star Mutation of Petunia xhybrida Vilm
J. Amer. Soc. Hort. Sci.,
September 1, 2007;
132(5):
680 - 690.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Ishida, S. Hattori, R. Sano, K. Inoue, Y. Shirano, H. Hayashi, D. Shibata, S. Sato, T. Kato, S. Tabata, et al.
Arabidopsis TRANSPARENT TESTA GLABRA2 Is Directly Regulated by R2R3 MYB Transcription Factors and Is Involved in Regulation of GLABRA2 Transcription in Epidermal Differentiation
PLANT CELL,
August 1, 2007;
19(8):
2531 - 2543.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Hanumappa, G. Choi, S. Ryu, and G. Choi
Modulation of flower colour by rationally designed dominant-negative chalcone synthase
J. Exp. Bot.,
July 1, 2007;
58(10):
2471 - 2478.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Ban, C. Honda, Y. Hatsuyama, M. Igarashi, H. Bessho, and T. Moriguchi
Isolation and Functional Analysis of a MYB Transcription Factor Gene that is a Key Regulator for the Development of Red Coloration in Apple Skin
Plant Cell Physiol.,
July 1, 2007;
48(7):
958 - 970.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Dombrecht, G. P. Xue, S. J. Sprague, J. A. Kirkegaard, J. J. Ross, J. B. Reid, G. P. Fitt, N. Sewelam, P. M. Schenk, J. M. Manners, et al.
MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis
PLANT CELL,
July 1, 2007;
19(7):
2225 - 2245.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Kleine, P. Kindgren, C. Benedict, L. Hendrickson, and A. Strand
Genome-Wide Gene Expression Analysis Reveals a Critical Role for CRYPTOCHROME1 in the Response of Arabidopsis to High Irradiance
Plant Physiology,
July 1, 2007;
144(3):
1391 - 1406.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. W. Jaffe, A. Tattersall, and B. J. Glover
A truncated MYB transcription factor from Antirrhinum majus regulates epidermal cell outgrowth
J. Exp. Bot.,
April 1, 2007;
58(6):
1515 - 1524.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Mano, F. Ogasawara, K. Sato, H. Higo, and Y. Minobe
Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet Potato
Plant Physiology,
March 1, 2007;
143(3):
1252 - 1268.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Friedmann, S. G. Ralph, D. Aeschliman, J. Zhuang, K. Ritland, B. E. Ellis, J. Bohlmann, and C. J. Douglas
Microarray gene expression profiling of developmental transitions in Sitka spruce (Picea sitchensis) apical shoots
J. Exp. Bot.,
February 1, 2007;
58(3):
593 - 614.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Inui, K.-i. Tamura, N. Fujii, T. Morishige, and F. Sato
Overexpression of Coptis japonica Norcoclaurine 6-O-Methyltransferase Overcomes the Rate-Limiting Step in Benzylisoquinoline Alkaloid Biosynthesis in Cultured Eschscholzia californica
Plant Cell Physiol.,
February 1, 2007;
48(2):
252 - 262.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Yang, Z. Xu, J. Song, K. Conner, G. Vizcay Barrena, and Z. A. Wilson
Arabidopsis MYB26/MALE STERILE35 Regulates Secondary Thickening in the Endothecium and Is Essential for Anther Dehiscence
PLANT CELL,
February 1, 2007;
19(2):
534 - 548.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. Rajagopalan, H. Vaucheret, J. Trejo, and D. P. Bartel
A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana
Genes & Dev.,
December 15, 2006;
20(24):
3407 - 3425.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. de Meaux, A. Pop, and T. Mitchell-Olds
Cis-regulatory Evolution of Chalcone-Synthase Expression in the Genus Arabidopsis
Genetics,
December 1, 2006;
174(4):
2181 - 2202.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. M. Takos, F. W. Jaffe, S. R. Jacob, J. Bogs, S. P. Robinson, and A. R. Walker
Light-Induced Expression of a MYB Gene Regulates Anthocyanin Biosynthesis in Red Apples
Plant Physiology,
November 1, 2006;
142(3):
1216 - 1232.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. A. Hannah, D. Wiese, S. Freund, O. Fiehn, A. G. Heyer, and D. K. Hincha
Natural Genetic Variation of Freezing Tolerance in Arabidopsis
Plant Physiology,
September 1, 2006;
142(1):
98 - 112.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N Wuyts, G Lognay, R Swennen, and D De Waele
Nematode infection and reproduction in transgenic and mutant Arabidopsis and tobacco with an altered phenylpropanoid metabolism
J. Exp. Bot.,
August 1, 2006;
57(11):
2825 - 2835.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Lu, Y. Zhou, L. Li, and V. L. Chiang
Distinct Roles of Cinnamate 4-hydroxylase Genes in Populus
Plant Cell Physiol.,
July 1, 2006;
47(7):
905 - 914.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. Quattrocchio, W. Verweij, A. Kroon, C. Spelt, J. Mol, and R. Koes
PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin Pathway
PLANT CELL,
May 1, 2006;
18(5):
1274 - 1291.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Morita, M. Saitoh, A. Hoshino, E. Nitasaka, and S. Iida
Isolation of cDNAs for R2R3-MYB, bHLH and WDR Transcriptional Regulators and Identification of c and ca Mutations Conferring White Flowers in the Japanese Morning Glory
Plant Cell Physiol.,
April 1, 2006;
47(4):
457 - 470.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Schwinn, J. Venail, Y. Shang, S. Mackay, V. Alm, E. Butelli, R. Oyama, P. Bailey, K. Davies, and C. Martin
A Small Family of MYB-Regulatory Genes Controls Floral Pigmentation Intensity and Patterning in the Genus Antirrhinum
PLANT CELL,
April 1, 2006;
18(4):
831 - 851.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Li, K. K. Lee, S. Walsh, C. Smith, S. Hadingham, K. Sorefan, G. Cawley, and M. W. Bevan
Establishing glucose- and ABA-regulated transcription networks in Arabidopsis by microarray analysis and promoter classification using a Relevance Vector Machine
Genome Res.,
March 1, 2006;
16(3):
414 - 427.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. van der Rest, S. Danoun, A.-M. Boudet, and S. F. Rochange
Down-regulation of cinnamoyl-CoA reductase in tomato (Solanum lycopersicum L.) induces dramatic changes in soluble phenolic pools
J. Exp. Bot.,
March 1, 2006;
57(6):
1399 - 1411.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Deluc, F. Barrieu, C. Marchive, V. Lauvergeat, A. Decendit, T. Richard, J.-P. Carde, J.-M. Merillon, and S. Hamdi
Characterization of a Grapevine R2R3-MYB Transcription Factor That Regulates the Phenylpropanoid Pathway
Plant Physiology,
February 1, 2006;
140(2):
499 - 511.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Solfanelli, A. Poggi, E. Loreti, A. Alpi, and P. Perata
Sucrose-Specific Induction of the Anthocyanin Biosynthetic Pathway in Arabidopsis
Plant Physiology,
February 1, 2006;
140(2):
637 - 646.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Lazar and H. M. Goodman
From The Cover: MAX1, a regulator of the flavonoid pathway, controls vegetative axillary bud outgrowth in Arabidopsis
PNAS,
January 10, 2006;
103(2):
472 - 476.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Bogs, A. Ebadi, D. McDavid, and S. P. Robinson
Identification of the Flavonoid Hydroxylases from Grapevine and Their Regulation during Fruit Development
Plant Physiology,
January 1, 2006;
140(1):
279 - 291.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Teng, J. Keurentjes, L. Bentsink, M. Koornneef, and S. Smeekens
Sucrose-Specific Induction of Anthocyanin Biosynthesis in Arabidopsis Requires the MYB75/PAP1 Gene
Plant Physiology,
December 1, 2005;
139(4):
1840 - 1852.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Vanderauwera, P. Zimmermann, S. Rombauts, S. Vandenabeele, C. Langebartels, W. Gruissem, D. Inze, and F. Van Breusegem
Genome-Wide Analysis of Hydrogen Peroxide-Regulated Gene Expression in Arabidopsis Reveals a High Light-Induced Transcriptional Cluster Involved in Anthocyanin Biosynthesis
Plant Physiology,
October 1, 2005;
139(2):
806 - 821.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Prassinos, J.-H. Ko, J. Yang, and K.-H. Han
Transcriptome Profiling of Vertical Stem Segments Provides Insights into the Genetic Regulation of Secondary Growth in Hybrid Aspen Trees
Plant Cell Physiol.,
August 1, 2005;
46(8):
1213 - 1225.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Y. Hirai, M. Klein, Y. Fujikawa, M. Yano, D. B. Goodenowe, Y. Yamazaki, S. Kanaya, Y. Nakamura, M. Kitayama, H. Suzuki, et al.
Elucidation of Gene-to-Gene and Metabolite-to-Gene Networks in Arabidopsis by Integration of Metabolomics and Transcriptomics
J. Biol. Chem.,
July 8, 2005;
280(27):
25590 - 25595.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. Sibout, A. Eudes, G. Mouille, B. Pollet, C. Lapierre, L. Jouanin, and A. Seguin
CINNAMYL ALCOHOL DEHYDROGENASE-C and -D Are the Primary Genes Involved in Lignin Biosynthesis in the Floral Stem of Arabidopsis
PLANT CELL,
July 1, 2005;
17(7):
2059 - 2076.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. Mehrtens, H. Kranz, P. Bednarek, and B. Weisshaar
The Arabidopsis Transcription Factor MYB12 Is a Flavonol-Specific Regulator of Phenylpropanoid Biosynthesis
Plant Physiology,
June 1, 2005;
138(2):
1083 - 1096.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. C. Verdonk, M. A. Haring, A. J. van Tunen, and R. C. Schuurink
ODORANT1 Regulates Fragrance Biosynthesis in Petunia Flowers
PLANT CELL,
May 1, 2005;
17(5):
1612 - 1624.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. de Meaux, U. Goebel, A. Pop, and T. Mitchell-Olds
Allele-Specific Assay Reveals Functional Variation in the Chalcone Synthase Promoter of Arabidopsis thaliana That Is Compatible with Neutral Evolution
PLANT CELL,
March 1, 2005;
17(3):
676 - 690.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Matsui, K. Hiratsu, T. Koyama, H. Tanaka, and M. Ohme-Takagi
A Chimeric AtMYB23 Repressor Induces Hairy Roots, Elongation of Leaves and Stems, and Inhibition of the Deposition of Mucilage on Seed Coats in Arabidopsis
Plant Cell Physiol.,
January 15, 2005;
46(1):
147 - 155.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. M. Voll, E. E. Allaire, G. Fiene, and A. P.M. Weber
The Arabidopsis phenylalanine insensitive growth Mutant Exhibits a Deregulated Amino Acid Metabolism
Plant Physiology,
October 1, 2004;
136(2):
3058 - 3069.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W.-R. Scheible, R. Morcuende, T. Czechowski, C. Fritz, D. Osuna, N. Palacios-Rojas, D. Schindelasch, O. Thimm, M. K. Udvardi, and M. Stitt
Genome-Wide Reprogramming of Primary and Secondary Metabolism, Protein Synthesis, Cellular Growth Processes, and the Regulatory Infrastructure of Arabidopsis in Response to Nitrogen
Plant Physiology,
September 1, 2004;
136(1):
2483 - 2499.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Kirst, A. A. Myburg, J. P.G. De Leon, M. E. Kirst, J. Scott, and R. Sederoff
Coordinated Genetic Regulation of Growth and Lignin Revealed by Quantitative Trait Locus Analysis of cDNA Microarray Data in an Interspecific Backcross of Eucalyptus
Plant Physiology,
August 1, 2004;
135(4):
2368 - 2378.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Pilot, H. Stransky, D. F. Bushey, R. Pratelli, U. Ludewig, V. P.M. Wingate, and W. B. Frommer
Overexpression of GLUTAMINE DUMPER1 Leads to Hypersecretion of Glutamine from Hydathodes of Arabidopsis Leaves
PLANT CELL,
July 1, 2004;
16(7):
1827 - 1840.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|