Plant Cell email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (28)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Essers, L.
Right arrow Articles by Kunze, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Essers, L.
Right arrow Articles by Kunze, R.
Agricola
Right arrow Articles by Essers, L.
Right arrow Articles by Kunze, R.
Plant Cell, Vol. 12, 211-224, February 2000, Copyright © 2000, American Society of Plant Physiologists

A Highly Conserved Domain of the Maize Activator Transposase Is Involved in Dimerization

Lutz Essersa, Ruth H. Adolphsa, and Reinhard Kunzea
a Institut für Genetik und Mikrobiologie, Universität München, Maria-Ward-Strasse 1a, 80638 Munich, Germany

Correspondence to: Reinhard Kunze, at Botanisches Institut, Universität zu Köln, Gyrhofstrasse 15, 50931 Cologne, Germany., reinhard.kunze{at}uni-koeln.de (E-mail), 49-221-470-5039 (fax)

Previous studies have presented indirect evidence that the transposase of the maize transposable element Activator (TPase) is active as an oligomer and forms inactive macromolecular complexes expressed in large amounts. Here, we have identified and characterized a dimerization domain at the C terminus of the protein. This domain is the most highly conserved region in the transposases of elements belonging to the Activator superfamily (hAT element superfamily) and contains a characteristic signature motif. The isolated dimerization domain forms extremely stable dimers in vitro. Interestingly, mutations in five of the six conserved residues of the signature motif do not affect in vitro dimerization, whereas mutations in other, less strictly conserved residues of the signature motif do. Loss of dimerization in vitro correlates with loss of TPase activity in vivo. As revealed by in situ immunofluorescence staining of mutant TPase proteins, the dimerization domain also is involved in forming inactive macromolecular aggregates when overexpressed, and the TPase contains one or more additional interaction functions.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. Lin, Y. Teng, H.-J. Park, L. Ding, C. Black, P. Fang, and H. Wang
Discrete and Essential Roles of the Multiple Domains of Arabidopsis FHY3 in Mediating Phytochrome A Signal Transduction
Plant Physiology, October 1, 2008; 148(2): 981 - 992.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Yamashita, H. Komori, Y. Higuchi, T. Yamaguchi, T. Osumi, and F. Hirose
Human DNA Replication-related Element Binding Factor (hDREF) Self-association via hATC Domain Is Necessary for Its Nuclear Accumulation and DNA Binding
J. Biol. Chem., March 9, 2007; 282(10): 7563 - 7575.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. A. Chesney, A. R. Kidd III, and J. Kimble
gon-14 Functions With Class B and Class C Synthetic Multivulva Genes to Control Larval Growth in Caenorhabditis elegans
Genetics, February 1, 2006; 172(2): 915 - 928.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
E. J. Pritham, C. Feschotte, and S. R. Wessler
Unexpected Diversity and Differential Success of DNA Transposons in Four Species of Entamoeba Protozoans
Mol. Biol. Evol., September 1, 2005; 22(9): 1751 - 1763.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Xu and H. K. Dooner
Mx-rMx, a Family of Interacting Transposons in the Growing hAT Superfamily of Maize
PLANT CELL, February 1, 2005; 17(2): 375 - 388.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
S. Souames, C. Bazin, E. Bonnivard, and D. Higuet
Behavior of the hobo Transposable Element with Regard to TPE Repeats in Transgenic Lines of Drosophila melanogaster
Mol. Biol. Evol., December 1, 2003; 20(12): 2055 - 2066.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. J. Pritham, Y. H. Zhang, C. Feschotte, and R. V. Kesseli
An Ac-like Transposable Element Family With Transcriptionally Active Y-Linked Copies in the White Campion, Silene latifolia
Genetics, October 1, 2003; 165(2): 799 - 807.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. M. Mack and N. M. Crawford
The Arabidopsis TAG1 Transposase Has an N-Terminal Zinc Finger DNA Binding Domain That Recognizes Distinct Subterminal Motifs
PLANT CELL, October 1, 2001; 13(10): 2319 - 2331.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. Rubin, G. Lithwick, and A. A. Levy
Structure and Evolution of the hAT Transposon Superfamily
Genetics, July 1, 2001; 158(3): 949 - 957.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
F. Ros and R. Kunze
Regulation of Activator/Dissociation Transposition by Replication and DNA Methylation
Genetics, April 1, 2001; 157(4): 1723 - 1733.
[Abstract] [Full Text]

Home page
 Plant CellHome page
G. N. Rudenko and V. Walbot
Expression and Post-Transcriptional Regulation of Maize Transposable Element MuDR and Its Derivatives
PLANT CELL, March 1, 2001; 13(3): 553 - 570.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2000 by the American Society of Plant Biologists