Plant Cell email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (64)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lacombe, B.
Right arrow Articles by Thibaud, J.-B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lacombe, B.
Right arrow Articles by Thibaud, J.-B.
Agricola
Right arrow Articles by Lacombe, B.
Right arrow Articles by Thibaud, J.-B.
Plant Cell, Vol. 12, 837-851, June 2000, Copyright © 2000, American Society of Plant Physiologists

A Shaker-like K+ Channel with Weak Rectification Is Expressed in Both Source and Sink Phloem Tissues of Arabidopsis

Benoît Lacombea, Guillaume Pilota, Erwan Micharda, Frédéric Gaymarda, Hervé Sentenaca, and Jean-Baptiste Thibauda
a Biochimie et Physiologie Moléculaire des Plantes, UMR 5004, Agro-M/CNRS/INRA/UM2, Place Viala, 34060 Montpellier Cedex 1, France

Correspondence to: Jean-Baptiste Thibaud, thibaud{at}ensam.inra.fr (E-mail), 33-467-52-57-37 (fax)

RNA gel blot and reverse transcription–polymerase chain reaction experiments were used to identify a single K+ channel gene in Arabidopsis as expressed throughout the plant. Use of the ß-glucuronidase reporter gene revealed expression of this gene, AKT2/AKT3, in both source and sink phloem tissues. The AKT2/AKT3 gene corresponds to two previously identified cDNAs, AKT2 (reconstructed at its 5' end) and AKT3, the open reading frame of the latter being shorter at its 5' end than that of the former. Rapid amplification of cDNA ends with polymerase chain reaction and site-directed mutagenesis was performed to identify the initiation codon for AKT2 translation. All of the data are consistent with the hypothesis that the encoded polypeptide corresponds to the longest open reading frame previously identified (AKT2). Electrophysiological characterization (macroscopic and single-channel currents) of AKT2 in both Xenopus oocytes and COS cells revealed a unique gating mode and sensitivity to pH (weak inward rectification, inhibition, and increased rectification upon internal or external acidification), suggesting that AKT2 has enough functional plasticity to perform different functions in phloem tissue of source and sink organs. The plant stress hormone abscisic acid was shown to increase the amount of AKT2 transcript, suggesting a role for the AKT2 in the plant response to drought.




This article has been cited by other articles:


Home page
 J Exp BotHome page
X. Y. Dai, Y. R. Su, W. X. Wei, J. S. Wu, and Y. K. Fan
Effects of top excision on the potassium accumulation and expression of potassium channel genes in tobacco
J. Exp. Bot., January 1, 2009; 60(1): 279 - 289.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Schmitt, R. Stadler, and N. Sauer
Immunolocalization of Solanaceous SUT1 Proteins in Companion Cells and Xylem Parenchyma: New Perspectives for Phloem Loading and Transport
Plant Physiology, September 1, 2008; 148(1): 187 - 199.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. C. Srivastava, S. Ganesan, I. O. Ismail, and B. G. Ayre
Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport
Plant Physiology, September 1, 2008; 148(1): 200 - 211.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. E. Ghanem, A. Albacete, C. Martinez-Andujar, M. Acosta, R. Romero-Aranda, I. C. Dodd, S. Lutts, and F. Perez-Alfocea
Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.)
J. Exp. Bot., August 1, 2008; 59(11): 3039 - 3050.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. B. Hafke, A. C.U. Furch, M. U. Reitz, and A. J.E. van Bel
Functional Sieve Element Protoplasts
Plant Physiology, November 1, 2007; 145(3): 703 - 711.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. C. Lee, W.-Z. Lan, B.-G. Kim, L. Li, Y. H. Cheong, G. K. Pandey, G. Lu, B. B. Buchanan, and S. Luan
A protein phosphorylation/dephosphorylation network regulates a plant potassium channel
PNAS, October 2, 2007; 104(40): 15959 - 15964.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. A. Khan, Q. Wang, R. D. Sjolund, A. Schulz, and G. A. Thompson
An Early Nodulin-Like Protein Accumulates in the Sieve Element Plasma Membrane of Arabidopsis
Plant Physiology, April 1, 2007; 143(4): 1576 - 1589.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Xicluna, B. Lacombe, I. Dreyer, C. Alcon, L. Jeanguenin, H. Sentenac, J.-B. Thibaud, and I. Cherel
Increased Functional Diversity of Plant K+ Channels by Preferential Heteromerization of the Shaker-like Subunits AKT2 and KAT2
J. Biol. Chem., January 5, 2007; 282(1): 486 - 494.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Shabala, V. Demidchik, L. Shabala, T. A. Cuin, S. J. Smith, A. J. Miller, J. M. Davies, and I. A. Newman
Extracellular Ca2+ Ameliorates NaCl-Induced K+ Loss from Arabidopsis Root and Leaf Cells by Controlling Plasma Membrane K+-Permeable Channels
Plant Physiology, August 1, 2006; 141(4): 1653 - 1665.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. K. Ashley, M. Grant, and A. Grabov
Plant responses to potassium deficiencies: a role for potassium transport proteins
J. Exp. Bot., January 1, 2006; 57(2): 425 - 436.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
E. Michard, B. Lacombe, F. Poree, B. Mueller-Roeber, H. Sentenac, J.-B. Thibaud, and I. Dreyer
A Unique Voltage Sensor Sensitizes the Potassium Channel AKT2 to Phosphoregulation
J. Gen. Physiol., November 28, 2005; 126(6): 605 - 617.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y.-H. Su, H. North, C. Grignon, J.-B. Thibaud, H. Sentenac, and A.-A. Very
Regulation by External K+ in a Maize Inward Shaker Channel Targets Transport Activity in the High Concentration Range
PLANT CELL, May 1, 2005; 17(5): 1532 - 1548.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
N. Ivashikina, R. Deeken, S. Fischer, P. Ache, and R. Hedrich
AKT2/3 Subunits Render Guard Cell K+ Channels Ca2+ Sensitive
J. Gen. Physiol., April 25, 2005; 125(5): 483 - 492.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. Cherel
Regulation of K+ channel activities in plants: from physiological to molecular aspects
J. Exp. Bot., February 1, 2004; 55(396): 337 - 351.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Philippar, K. Buchsenschutz, M. Abshagen, I. Fuchs, D. Geiger, B. Lacombe, and R. Hedrich
The K+ Channel KZM1 Mediates Potassium Uptake into the Phloem and Guard Cells of the C4 Grass Zea mays
J. Biol. Chem., May 2, 2003; 278(19): 16973 - 16981.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Desbrosses, C. Josefsson, S. Rigas, P. Hatzopoulos, and L. Dolan
AKT1 and TRH1 are required during root hair elongation in Arabidopsis
J. Exp. Bot., February 1, 2003; 54(383): 781 - 788.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Macri, C. Proenza, E. Agranovich, D. Angoli, and E. A. Accili
Separable Gating Mechanisms in a Mammalian Pacemaker Channel
J. Biol. Chem., September 20, 2002; 277(39): 35939 - 35946.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Geiger, D. Becker, B. Lacombe, and R. Hedrich
Outer Pore Residues Control the H+ and K+ Sensitivity of the Arabidopsis Potassium Channel AKT3
PLANT CELL, August 1, 2002; 14(8): 1859 - 1868.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
I. Cherel, E. Michard, N. Platet, K. Mouline, C. Alcon, H. Sentenac, and J.-B. Thibaud
Physical and Functional Interaction of the Arabidopsis K+ Channel AKT2 and Phosphatase AtPP2CA
PLANT CELL, May 1, 2002; 14(5): 1133 - 1146.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Reintanz, A. Szyroki, N. Ivashikina, P. Ache, M. Godde, D. Becker, K. Palme, and R. Hedrich
AtKC1, a silent Arabidopsis potassium channel alpha -subunit modulates root hair K+ influx
PNAS, March 19, 2002; 99(6): 4079 - 4084.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Proenza, D. Angoli, E. Agranovich, V. Macri, and E. A. Accili
Pacemaker Channels Produce an Instantaneous Current
J. Biol. Chem., February 8, 2002; 277(7): 5101 - 5109.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
K. Mouline, A.-A. Very, F. Gaymard, J. Boucherez, G. Pilot, M. Devic, D. Bouchez, J.-B. Thibaud, and H. Sentenac
Pollen tube development and competitive ability are impaired by disruption of a Shaker K+ channel in Arabidopsis
Genes & Dev., February 1, 2002; 16(3): 339 - 350.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Pratelli, B. Lacombe, L. Torregrosa, F. Gaymard, C. Romieu, J.-B. Thibaud, and H. Sentenac
A Grapevine Gene Encoding a Guard Cell K+ Channel Displays Developmental Regulation in the Grapevine Berry
Plant Physiology, February 1, 2002; 128(2): 564 - 577.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. L. Dennison, W. R. Robertson, B. D. Lewis, R. E. Hirsch, M. R. Sussman, and E. P. Spalding
Functions of AKT1 and AKT2 Potassium Channels Determined by Studies of Single and Double Mutants of Arabidopsis
Plant Physiology, November 1, 2001; 127(3): 1012 - 1019.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Yu, M. Moshelion, and N. Moran
Extracellular Protons Inhibit the Activity of Inward- Rectifying Potassium Channels in the Motor Cells of Samanea saman Pulvini
Plant Physiology, November 1, 2001; 127(3): 1310 - 1322.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. M. Kwak, Y. Murata, V. M. Baizabal-Aguirre, J. Merrill, M. Wang, A. Kemper, S. D. Hawke, G. Tallman, and J. I. Schroeder
Dominant Negative Guard Cell K+ Channel Mutants Reduce Inward-Rectifying K+ Currents and Light-Induced Stomatal Opening in Arabidopsis
Plant Physiology, October 1, 2001; 127(2): 473 - 485.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Uozumi
Escherichia coli as an expression system for K+ transport systems from plants
Am J Physiol Cell Physiol, September 1, 2001; 281(3): C733 - C739.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Liu, D. J. Fairbairn, R. J. Reid, and D. P. Schachtman
Characterization of Two HKT1 Homologues from Eucalyptus camaldulensis That Display Intrinsic Osmosensing Capability
Plant Physiology, September 1, 2001; 127(1): 283 - 294.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
J. C. Cushman
Osmoregulation in Plants: Implications for Agriculture
Integr. Comp. Biol., August 1, 2001; 41(4): 758 - 769.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Maser, S. Thomine, J. I. Schroeder, J. M. Ward, K. Hirschi, H. Sze, I. N. Talke, A. Amtmann, F. J.M. Maathuis, D. Sanders, et al.
Phylogenetic Relationships within Cation Transporter Families of Arabidopsis
Plant Physiology, August 1, 2001; 126(4): 1646 - 1667.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Hoth, D. Geiger, D. Becker, and R. Hedrich
The Pore of Plant K+ Channels Is Involved in Voltage and pH Sensing: Domain-Swapping between Different K+ Channel {{alpha}}-Subunits
PLANT CELL, April 1, 2001; 13(4): 943 - 952.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Szyroki, N. Ivashikina, P. Dietrich, M. R. G. Roelfsema, P. Ache, B. Reintanz, R. Deeken, M. Godde, H. Felle, R. Steinmeyer, et al.
KAT1 is not essential for stomatal opening
PNAS, February 15, 2001; (2001) 51616698.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. Pilot, B. Lacombe, F. Gaymard, I. Cherel, J. Boucherez, J.-B. Thibaud, and H. Sentenac
Guard Cell Inward K+ Channel Activity in Arabidopsis Involves Expression of the Twin Channel Subunits KAT1 and KAT2
J. Biol. Chem., January 26, 2001; 276(5): 3215 - 3221.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Szyroki, N. Ivashikina, P. Dietrich, M. R. G. Roelfsema, P. Ache, B. Reintanz, R. Deeken, M. Godde, H. Felle, R. Steinmeyer, et al.
KAT1 is not essential for stomatal opening
PNAS, February 27, 2001; 98(5): 2917 - 2921.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2000 by the American Society of Plant Biologists