|
Plant Cell, Vol. 12, 1127-1136, July 2000, Copyright © 2000, American Society of Plant Physiologists
Ca2+-Stimulated Exocytosis in Maize Coleoptile Cells
Jens-Uwe Suttera,
Ulrike Homanna, and
Gerhard Thiela
a Albrecht-von-Haller Institute for Plant Sciences, Plant Biophysics, Untere Karspüle 2, 37073 Göttingen, Germany
Correspondence to:
Gerhard Thiel, gthiel{at}gwdvms.dnet.gwdg.de (E-mail), 49-551-397838 (fax)
Changes in membrane capacitance (Cm) after photolysis of the caged Ca2+ compound dimethoxynitrophenamine were studied in protoplasts from maize coleoptiles. Changes in Cm values resulting from increased concentrations of free Ca2+ in the cytoplasm ([Ca2+]cyt) were interpreted as representing changes in [Ca2+]cyt–sensitive exocytosis and endocytosis. A continuous increase in [Ca2+]cyt resulted in a sigmoidal increase in Cm values with a half-maximal concentration at ~1 µM. The steep increase in Cm values was followed by a variable slow phase in changing Cm values. When [Ca2+]cyt increased at a rate of 0.6 µmol L-1 sec-1, the initial steep increase in Cm values lasted ~5 to 10 sec. During this time, protoplasts increased in surface area by ~2.5%. The biphasic dynamics of [Ca2+]cyt–stimulated increases in Cm values can be described by a kinetic model containing two pools of vesicles with two [Ca2+]cyt–sensitive steps in the exocytotic pathway.
This article has been cited by other articles:
|
|
|
|
 
S. Sokolovski, A. Hills, R. A. Gay, and M. R. Blatt
Functional Interaction of the SNARE Protein NtSyp121 in Ca2+ Channel Gating, Ca2+ Transients and ABA Signalling of Stomatal Guard Cells
Mol Plant,
March 1, 2008;
1(2):
347 - 358.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Campanoni and M. R. Blatt
Membrane trafficking and polar growth in root hairs and pollen tubes
J. Exp. Bot.,
January 1, 2007;
58(1):
65 - 74.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-U. Sutter, P. Campanoni, M. Tyrrell, and M. R. Blatt
Selective Mobility and Sensitivity to SNAREs Is Exhibited by the Arabidopsis KAT1 K+ Channel at the Plasma Membrane
PLANT CELL,
April 1, 2006;
18(4):
935 - 954.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Moshelion, N. Moran, and F. Chaumont
Dynamic Changes in the Osmotic Water Permeability of Protoplast Plasma Membrane
Plant Physiology,
August 1, 2004;
135(4):
2301 - 2317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. S. Nuhse, T. Boller, and S. C. Peck
A Plasma Membrane Syntaxin Is Phosphorylated in Response to the Bacterial Elicitor Flagellin
J. Biol. Chem.,
November 14, 2003;
278(46):
45248 - 45254.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Geelen, B. Leyman, H. Batoko, G.-P. Di Sansebastiano, I. Moore, and M. R. Blatt
The Abscisic Acid-Related SNARE Homolog NtSyr1 Contributes to Secretion and Growth: Evidence from Competition with Its Cytosolic Domain
PLANT CELL,
February 1, 2002;
14(2):
387 - 406.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Long and M. Iino
Light-Dependent Osmoregulation in Pea Stem Protoplasts. Photoreceptors, Tissue Specificity, Ion Relationships, and Physiological Implications
Plant Physiology,
April 1, 2001;
125(4):
1854 - 1869.
[Abstract]
[Full Text]
|
|
|
|
|
