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Plant Cell, Vol. 12, 1127-1136, July 2000, Copyright © 2000, American Society of Plant Physiologists
Ca2+-Stimulated Exocytosis in Maize Coleoptile Cells
Jens-Uwe Suttera,
Ulrike Homanna, and
Gerhard Thiela
a Albrecht-von-Haller Institute for Plant Sciences, Plant Biophysics, Untere Karspüle 2, 37073 Göttingen, Germany
Correspondence to:
Gerhard Thiel, gthiel{at}gwdvms.dnet.gwdg.de (E-mail), 49-551-397838 (fax)
Changes in membrane capacitance (Cm) after photolysis of the caged Ca2+ compound dimethoxynitrophenamine were studied in protoplasts from maize coleoptiles. Changes in Cm values resulting from increased concentrations of free Ca2+ in the cytoplasm ([Ca2+]cyt) were interpreted as representing changes in [Ca2+]cyt–sensitive exocytosis and endocytosis. A continuous increase in [Ca2+]cyt resulted in a sigmoidal increase in Cm values with a half-maximal concentration at ~1 µM. The steep increase in Cm values was followed by a variable slow phase in changing Cm values. When [Ca2+]cyt increased at a rate of 0.6 µmol L-1 sec-1, the initial steep increase in Cm values lasted ~5 to 10 sec. During this time, protoplasts increased in surface area by ~2.5%. The biphasic dynamics of [Ca2+]cyt–stimulated increases in Cm values can be described by a kinetic model containing two pools of vesicles with two [Ca2+]cyt–sensitive steps in the exocytotic pathway.
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