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Plant Cell, Vol. 13, 101-111, January 2001, Copyright © 2001, American Society of Plant Physiologists

CYP83B1, a Cytochrome P450 at the Metabolic Branch Point in Auxin and Indole Glucosinolate Biosynthesis in Arabidopsis

Søren Baka, Frans E. Taxa, Kenneth A. Feldmannb, David W. Galbraitha, and René Feyereisena
a Departments of Plant Sciences, Molecular and Cellular Biology, and Entomology, University of Arizona, Tucson, Arizona 85721
b Ceres, Inc., 3007 Malibu Canyon Road, Malibu, California 90265

Correspondence to: René Feyereisen, at INRA Centre de Recherches d'Antibes, 1382 Route de Biot, 06560 Valbonne, France., rfeyer{at}salis.antibes.inra.fr (E-mail), 33-04-93-12-25-28 (fax)

Auxins are growth regulators involved in virtually all aspects of plant development. However, little is known about how plants synthesize these essential compounds. We propose that the level of indole-3-acetic acid is regulated by the flux of indole-3-acetaldoxime through a cytochrome P450, CYP83B1, to the glucosinolate pathway. A T-DNA insertion in the CYP83B1 gene leads to plants with a phenotype that suggests severe auxin overproduction, whereas CYP83B1 overexpression leads to loss of apical dominance typical of auxin deficit. CYP83B1 N-hydroxylates indole-3-acetaldoxime to the corresponding aci-nitro compound, 1-aci-nitro-2-indolyl-ethane, with a Km of 3 µM and a turnover number of 53 min-1. The aci-nitro compound formed reacts non-enzymatically with thiol compounds to produce an N-alkyl-thiohydroximate adduct, the committed precursor of glucosinolates. Thus, indole-3-acetaldoxime is the metabolic branch point between the primary auxin indole-3-acetic acid and indole glucosinolate biosynthesis in Arabidopsis.


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