Plant Cell Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (52)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wulff, B. B. H.
Right arrow Articles by Jones, J. D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wulff, B. B. H.
Right arrow Articles by Jones, J. D. G.
Agricola
Right arrow Articles by Wulff, B. B. H.
Right arrow Articles by Jones, J. D. G.
Plant Cell, Vol. 13, 255-272, February 2001, Copyright © 2001, American Society of Plant Physiologists

Domain Swapping and Gene Shuffling Identify Sequences Required for Induction of an Avr-Dependent Hypersensitive Response by the Tomato Cf-4 and Cf-9 Proteins

Brande B. H. Wulffa, Colwyn M. Thomasa, Matthew Smokera, Murray Granta, and Jonathan D. G. Jonesa
a Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, United Kingdom

Correspondence to: Jonathan D. G. Jones, jonathan.jones{at}bbsrc.ac.uk (E-mail), 44-1603-450011 (fax)

The tomato Cf-4 and Cf-9 genes confer resistance to infection by the biotrophic leaf mold pathogen Cladosporium. Their protein products induce a hypersensitive response (HR) upon recognition of the fungus-encoded Avr4 and Avr9 peptides. Cf-4 and Cf-9 share >91% sequence identity and are distinguished by sequences in their N-terminal domains A and B, their N-terminal leucine-rich repeats (LRRs) in domain C1, and their LRR copy number (25 and 27 LRRs, respectively). Analysis of Cf-4/Cf-9 chimeras, using several different bioassays, has identified sequences in Cf-4 and Cf-9 that are required for the Avr-dependent HR in tobacco and tomato. A 10–amino acid deletion within Cf-4 domain B relative to Cf-9 was required for full Avr4-dependent induction of an HR in most chimeras analyzed. Additional sequences required for Cf-4 function are located in LRRs 11 and 12, a region that contains only eight of the 67 amino acids that distinguish it from Cf-9. One chimera, with 25 LRRs that retained LRR 11 of Cf-4, induced an attenuated Avr4-dependent HR. The substitution of Cf-9 N-terminal LRRs 1 to 9 with the corresponding sequences from Cf-4 resulted in attenuation of the Avr9-induced HR, as did substitution of amino acid A433 in LRR 15. The amino acids L457 and K511 in Cf-9 LRRs 16 and 18 are essential for induction of the Avr9-dependent HR. Therefore, important sequence determinants of Cf-9 function are located in LRRs 10 to 18. This region contains 15 of the 67 amino acids that distinguish it from Cf-4, in addition to two extra LRRs. Our results demonstrate that sequence variation within the central LRRs of domain C1 and variation in LRR copy number in Cf-4 and Cf-9 play a major role in determining recognition specificity in these proteins.




This article has been cited by other articles:


Home page
 Plant CellHome page
F. M. Dunning, W. Sun, K. L. Jansen, L. Helft, and A. F. Bent
Identification and Mutational Analysis of Arabidopsis FLS2 Leucine-Rich Repeat Domain Residues That Contribute to Flagellin Perception
PLANT CELL, October 1, 2007; 19(10): 3297 - 3313.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
X. Liu, F. Lin, L. Wang, and Q. Pan
The in Silico Map-Based Cloning of Pi36, a Rice Coiled-Coil Nucleotide-Binding Site Leucine-Rich Repeat Gene That Confers Race-Specific Resistance to the Blast Fungus
Genetics, August 1, 2007; 176(4): 2541 - 2549.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Heese, A. A. Ludwig, and J. D.G. Jones
Rapid Phosphorylation of a Syntaxin during the Avr9/Cf-9-Race-Specific Signaling Pathway
Plant Physiology, August 1, 2005; 138(4): 2406 - 2416.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Bernal, Q. Pan, J. Pollack, L. Rose, A. Kozik, N. Willits, Y. Luo, M. Guittet, E. Kochetkova, and R. W. Michelmore
Functional Analysis of the Plant Disease Resistance Gene Pto Using DNA Shuffling
J. Biol. Chem., June 17, 2005; 280(24): 23073 - 23083.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. K. Fritz-Laylin, N. Krishnamurthy, M. Tor, K. V. Sjolander, and J. D.G. Jones
Phylogenomic Analysis of the Receptor-Like Proteins of Rice and Arabidopsis
Plant Physiology, June 1, 2005; 138(2): 611 - 623.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. A.L. van der Hoorn, B. B.H. Wulff, S. Rivas, M. C. Durrant, A. van der Ploeg, P. J.G.M. de Wit, and J. D.G. Jones
Structure-Function Analysis of Cf-9, a Receptor-Like Protein with Extracytoplasmic Leucine-Rich Repeats
PLANT CELL, March 1, 2005; 17(3): 1000 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. L. Caicedo and B. A. Schaal
Heterogeneous evolutionary processes affect R gene diversity in natural populations of Solanum pimpinellifolium
PNAS, December 14, 2004; 101(50): 17444 - 17449.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Kruijt, B. F. Brandwagt, and P. J. G. M. de Wit
Rearrangements in the Cf-9 Disease Resistance Gene Cluster of Wild Tomato Have Resulted in Three Genes That Mediate Avr9 Responsiveness
Genetics, November 1, 2004; 168(3): 1655 - 1663.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
B. B. H. Wulff, C. M. Thomas, M. Parniske, and J. D. G. Jones
Genetic Variation at the Tomato Cf-4/Cf-9 Locus Induced by EMS Mutagenesis and Intralocus Recombination
Genetics, May 1, 2004; 167(1): 459 - 470.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. E. Rose, P. D. Bittner-Eddy, C. H. Langley, E. B. Holub, R. W. Michelmore, and J. L. Beynon
The Maintenance of Extreme Amino Acid Diversity at the Disease Resistance Gene, RPP13, in Arabidopsis thaliana
Genetics, March 1, 2004; 166(3): 1517 - 1527.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Song, J. M. Bradeen, S. K. Naess, J. A. Raasch, S. M. Wielgus, G. T. Haberlach, J. Liu, H. Kuang, S. Austin-Phillips, C. R. Buell, et al.
Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight
PNAS, August 5, 2003; 100(16): 9128 - 9133.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Q.-H. Shen, F. Zhou, S. Bieri, T. Haizel, K. Shirasu, and P. Schulze-Lefert
Recognition Specificity and RAR1/SGT1 Dependence in Barley Mla Disease Resistance Genes to the Powdery Mildew Fungus
PLANT CELL, March 1, 2003; 15(3): 732 - 744.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Fluhr
Sentinels of Disease. Plant Resistance Genes
Plant Physiology, December 1, 2001; 127(4): 1367 - 1374.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
F. Taguchi-Shiobara, Z. Yuan, S. Hake, and D. Jackson
The fasciated ear2 gene encodes a leucine-rich repeat receptor-like protein that regulates shoot meristem proliferation in maize
Genes & Dev., October 15, 2001; 15(20): 2755 - 2766.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. A. L. Van der Hoorn, M. Kruijt, R. Roth, B. F. Brandwagt, M. H. A. J. Joosten, and P. J. G. M. De Wit
Intragenic recombination generated two distinct Cf genes that mediate AVR9 recognition in the natural population of Lycopersiconpimpinellifolium
PNAS, August 17, 2001; (2001) 181241798.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. A. L. Van der Hoorn, M. Kruijt, R. Roth, B. F. Brandwagt, M. H. A. J. Joosten, and P. J. G. M. De Wit
Intragenic recombination generated two distinct Cf genes that mediate AVR9 recognition in the natural population of Lycopersiconpimpinellifolium
PNAS, August 28, 2001; 98(18): 10493 - 10498.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Rivas, T. Romeis, and J. D. G. Jones
The Cf-9 Disease Resistance Protein Is Present in an ~420-Kilodalton Heteromultimeric Membrane-Associated Complex at One Molecule per Complex
PLANT CELL, March 1, 2002; 14(3): 689 - 702.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2001 by the American Society of Plant Biologists