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Plant Cell, Vol. 13, 319-335, February 2001, Copyright © 2001, American Society of Plant Physiologists

Transgene-Induced Silencing Identifies Sequences Involved in the Establishment of Paramutation of the Maize p1 Gene

Lyudmila V. Sidorenkoa and Thomas Petersona
a Department of Zoology and Genetics and Department of Agronomy, Iowa State University, Ames, Iowa 50011

Correspondence to: Thomas Peterson, thomasp{at}iastate.edu (E-mail), 515-294-6755 (fax)

A transgene carrying a distal enhancer element of the maize P1-rr promoter caused silencing of an endogenous P1-rr allele in the progeny of transgenic maize plants. Expression of both the transgene and the endogenous P1-rr allele was reduced in the affected plants. The silenced phenotype was observed in the progeny of seven of eight crosses involving three independent transgenic events tested (average frequency of 19%). This phenotype was associated with an induced epigenetic state of the P1-rr allele, termed P1-rr', which is characterized by increased methylation of the P1-rr flanking regions and decreased levels of P1-rr transcript. The P1-rr' epiallele is highly heritable in the absence of the inducing P1.2b::GUS transgene, and it can impose an equivalent state on a naive P1-rr allele in subsequent crosses (paramutation). In contrast, parallel experiments with two other P::GUS transgenes that contained the same basal P1-rr promoter fragment but different upstream sequences revealed no detectable silencing effect. Thus, transgenes carrying a specific enhancer fragment of the P1-rr gene promoter can trigger a paramutant state (P1-rr') of the endogenous P1-rr gene that is maintained in the absence of the inducing transgene. We discuss the potential role of the P1-rr distal enhancer element in the establishment and propagation of a paramutation system in maize.




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