Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (58)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Viola, R.
Right arrow Articles by Oparka, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Viola, R.
Right arrow Articles by Oparka, K. J.
Agricola
Right arrow Articles by Viola, R.
Right arrow Articles by Oparka, K. J.
Plant Cell, Vol. 13, 385-398, February 2001, Copyright © 2001, American Society of Plant Physiologists

Tuberization in Potato Involves a Switch from Apoplastic to Symplastic Phloem Unloading

Roberto Violaa, Alison G. Robertsb, Sophie Hauptb, Silvia Gazzania,c, Robert D. Hancocka, Nelson Marmirolic, Gordon C. Machrayd, and Karl J. Oparkab
a Unit of Plant Biochemistry, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom
b Unit of Cell Biology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom
c Department of Evolutionary Biology, University of Parma, Viale delle Scienze, 43100 Parma, Italy
d Unit of Gene Expression, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom

Correspondence to: Roberto Viola, rviola{at}scri.sari.ac.uk (E-mail), 44-1382-562-426 (fax)

Phloem unloading was studied in potato plants in real time during the early stages of tuberization using carboxyfluorescein (CF) as a phloem-mobile tracer, and the unloading pattern was compared with autoradiography of tubers that had transported 14C assimilates. In stolons undergoing extension growth, apoplastic phloem unloading predominated. However, during the first visible signs of tuberization, a transition occurred from apoplastic to symplastic transport, and both CF and 14C assimilates subsequently followed identical patterns of phloem unloading. It is suggested that the switch to symplastic sucrose unloading may be responsible for the upregulation of several genes involved in sucrose metabolism. A detailed analysis of sugar levels and 14C sugar partitioning in tuberizing stolons revealed a distinct difference between the apical region of the tuber and the subapical region. Analysis of invertase activity in nontuberizing and tuberizing stolons revealed a marked decline in soluble invertase in the subapical region of swelling stolons, consistent with the switch from apoplastic to symplastic unloading. However, cell wall–bound invertase activity remained high in the apical 1 to 2 mm of tuberizing stolons. Histochemical analysis of potato lines transformed with the promoter of an apoplastic invertase gene (invGE) linked to a reporter gene also revealed discrete gene expression in the apical bud region. Evidence is presented that the apical and lateral tuber buds function as isolated domains with respect to sucrose unloading and metabolism.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. Fettke, A. Nunes-Nesi, J. Alpers, M. Szkop, A. R. Fernie, and M. Steup
Alterations in Cytosolic Glucose-Phosphate Metabolism Affect Structural Features and Biochemical Properties of Starch-Related Heteroglycans
Plant Physiology, November 1, 2008; 148(3): 1614 - 1629.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Riewe, L. Grosman, H. Zauber, C. Wucke, A. R. Fernie, and P. Geigenberger
Metabolic and Developmental Adaptations of Growing Potato Tubers in Response to Specific Manipulations of the Adenylate Energy Status
Plant Physiology, April 1, 2008; 146(4): 1579 - 1598.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. N. Oliver, A. Tiessen, A. R. Fernie, and P. Geigenberger
Decreased expression of plastidial adenylate kinase in potato tubers results in an enhanced rate of respiration and a stimulation of starch synthesis that is attributable to post-translational redox-activation of ADP-glucose pyrophosphorylase
J. Exp. Bot., February 4, 2008; (2008) erm312v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Kronberg, F. Vogel, T. Rutten, M.-R. Hajirezaei, U. Sonnewald, and D. Hofius
The Silver Lining of a Viral Agent: Increasing Seed Yield and Harvest Index in Arabidopsis by Ectopic Expression of the Potato Leaf Roll Virus Movement Protein
Plant Physiology, November 1, 2007; 145(3): 905 - 918.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X.-Y. Zhang, X.-L. Wang, X.-F. Wang, G.-H. Xia, Q.-H. Pan, R.-C. Fan, F.-Q. Wu, X.-C. Yu, and D.-P. Zhang
A Shift of Phloem Unloading from Symplasmic to Apoplasmic Pathway Is Involved in Developmental Onset of Ripening in Grape Berry
Plant Physiology, September 1, 2006; 142(1): 220 - 232.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Baroja-Fernandez, E. Etxeberria, F. J. Munoz, M. T. Moran-Zorzano, N. Alonso-Casajus, P. Gonzalez, and J. Pozueta-Romero
An Important Pool of Sucrose Linked to Starch Biosynthesis is Taken up by Endocytosis in Heterotrophic Cells
Plant Cell Physiol., April 1, 2006; 47(4): 447 - 456.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. I. Sergeeva, J. J. B. Keurentjes, L. Bentsink, J. Vonk, L. H. W. van der Plas, M. Koornneef, and D. Vreugdenhil
Vacuolar invertase regulates elongation of Arabidopsis thaliana roots as revealed by QTL and mutant analysis
PNAS, February 21, 2006; 103(8): 2994 - 2999.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
B. H. Junker, R. Wuttke, A. Nunes-Nesi, D. Steinhauser, N. Schauer, D. Bussis, L. Willmitzer, and A. R. Fernie
Enhancing Vacuolar Sucrose Cleavage Within the Developing Potato Tuber has only Minor Effects on Metabolism
Plant Cell Physiol., February 1, 2006; 47(2): 277 - 289.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. V. Davies, L. V. T. Shepherd, M. M. Burrell, F. Carrari, E. Urbanczyk-Wochniak, A. Leisse, R. D. Hancock, M. Taylor, R. Viola, H. Ross, et al.
Modulation of Fructokinase Activity of Potato (Solanum tuberosum) Results in Substantial Shifts in Tuber Metabolism
Plant Cell Physiol., July 1, 2005; 46(7): 1103 - 1115.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Carpaneto, D. Geiger, E. Bamberg, N. Sauer, J. Fromm, and R. Hedrich
Phloem-localized, Proton-coupled Sucrose Carrier ZmSUT1 Mediates Sucrose Efflux under the Control of the Sucrose Gradient and the Proton Motive Force
J. Biol. Chem., June 3, 2005; 280(22): 21437 - 21443.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. Li, J. Strahwald, H.-R. Hofferbert, J. Lubeck, E. Tacke, H. Junghans, J. Wunder, and C. Gebhardt
DNA Variation at the Invertase Locus invGE/GF Is Associated With Tuber Quality Traits in Populations of Potato Breeding Clones
Genetics, June 1, 2005; 170(2): 813 - 821.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G.-L. Wu, X.-Y. Zhang, L.-Y. Zhang, Q.-H. Pan, Y.-Y. Shen, and D.-P. Zhang
Phloem Unloading in Developing Walnut Fruit is Symplasmic in the Seed Pericarp and Apoplasmic in the Fleshy Pericarp
Plant Cell Physiol., October 15, 2004; 45(10): 1461 - 1470.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. L. Tomlinson, S. McHugh, H. Labbe, J. L. Grainger, L. E. James, K. M. Pomeroy, J. W. Mullin, S. S. Miller, D. T. Dennis, and B. L. A. Miki
Evidence that the hexose-to-sucrose ratio does not control the switch to storage product accumulation in oilseeds: analysis of tobacco seed development and effects of overexpressing apoplastic invertase
J. Exp. Bot., October 1, 2004; 55(406): 2291 - 2303.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L.-Y. Zhang, Y.-B. Peng, S. Pelleschi-Travier, Y. Fan, Y.-F. Lu, Y.-M. Lu, X.-P. Gao, Y.-Y. Shen, S. Delrot, and D.-P. Zhang
Evidence for Apoplasmic Phloem Unloading in Developing Apple Fruit
Plant Physiology, May 1, 2004; 135(1): 574 - 586.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Complainville, L. Brocard, I. Roberts, E. Dax, N. Sever, N. Sauer, A. Kondorosi, S. Wolf, K. Oparka, and M. Crespi
Nodule Initiation Involves the Creation of a New Symplasmic Field in Specific Root Cells of Medicago Species
PLANT CELL, December 1, 2003; 15(12): 2778 - 2791.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Raices, R. M. Ulloa, G. C. MacIntosh, M. Crespi, and M. T. Tellez-Inon
StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration
J. Exp. Bot., November 1, 2003; 54(392): 2589 - 2591.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
U. Roessner-Tunali, B. Hegemann, A. Lytovchenko, F. Carrari, C. Bruedigam, D. Granot, and A. R. Fernie
Metabolic Profiling of Transgenic Tomato Plants Overexpressing Hexokinase Reveals That the Influence of Hexose Phosphorylation Diminishes during Fruit Development
Plant Physiology, September 1, 2003; 133(1): 84 - 99.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. CENZANO, A. VIGLIOCCO, T. KRAUS, and G. ABDALA
Exogenously Applied Jasmonic Acid Induces Changes in Apical Meristem Morphology of Potato Stolons
Ann. Bot., June 1, 2003; 91(7): 915 - 919.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. J.E. van Bel
Transport Phloem: Low Profile, High Impact
Plant Physiology, April 1, 2003; 131(4): 1509 - 1510.
[Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
C.-T. CHEN and T. L. SETTER
Response of Potato Tuber Cell Division and Growth to Shade and Elevated CO2
Ann. Bot., February 1, 2003; 91(3): 373 - 381.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Fridman and D. Zamir
Functional Divergence of a Syntenic Invertase Gene Family in Tomato, Potato, and Arabidopsis
Plant Physiology, February 1, 2003; 131(2): 603 - 609.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Geigenberger
Regulation of sucrose to starch conversion in growing potato tubers
J. Exp. Bot., January 3, 2003; 54(382): 457 - 465.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M.-R. Hajirezaei, F. Bornke, M. Peisker, Y. Takahata, J. Lerchl, A. Kirakosyan, and U. Sonnewald
Decreased sucrose content triggers starch breakdown and respiration in stored potato tubers (Solanum tuberosum)
J. Exp. Bot., January 3, 2003; 54(382): 477 - 488.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. M. Sherson, H. L. Alford, S. M. Forbes, G. Wallace, and S. M. Smith
Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis
J. Exp. Bot., January 3, 2003; 54(382): 525 - 531.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Kuhn, M.-R. Hajirezaei, A. R. Fernie, U. Roessner-Tunali, T. Czechowski, B. Hirner, and W. B. Frommer
The Sucrose Transporter StSUT1 Localizes to Sieve Elements in Potato Tuber Phloem and Influences Tuber Physiology and Development
Plant Physiology, January 1, 2003; 131(1): 102 - 113.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. R. Fernie and L. Willmitzer
Molecular and Biochemical Triggers of Potato Tuber Development
Plant Physiology, December 1, 2001; 127(4): 1459 - 1465.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2001 by the American Society of Plant Biologists