|
Plant Cell, Vol. 13, 755-768, April 2001, Copyright © 2001, American Society of Plant Physiologists
A Cell Plate–Specific Callose Synthase and Its Interaction with Phragmoplastin
Zonglie Hong,
Ashton J. Delauney, and
Desh Pal S. Verma
Department of Molecular Genetics and Plant Biotechnology Center, Ohio State University, 1060 Carmack Road, Columbus, Ohio 43210
Correspondence to:
Desh Pal S. Verma, verma.1{at}osu.edu (E-mail), 614-292-5379 (fax)
Callose is synthesized on the forming cell plate and several other locations in the plant. We cloned an Arabidopsis cDNA encoding a callose synthase (CalS1) catalytic subunit. The CalS1 gene comprises 42 exons with 41 introns and is transcribed into a 6.0-kb mRNA. The deduced peptide, with an approximate molecular mass of 226 kD, showed sequence homology with the yeast 1,3-ß-glucan synthases and is distinct from plant cellulose synthases. CalS1 contains 16 predicted transmembrane helices with the N-terminal region and a large central loop facing the cytoplasm. CalS1 interacts with two cell plate–associated proteins, phragmoplastin and a novel UDP-glucose transferase that copurifies with the CalS complex. That CalS1 is a cell plate–specific enzyme is demonstrated by the observations that the green fluorescent protein–CalS1 fusion protein was localized at the growing cell plate, that expression of CalS1 in transgenic tobacco cells enhanced callose synthesis on the forming cell plate, and that these cell lines exhibited higher levels of CalS activity. These data also suggest that plant CalS may form a complex with UDP-glucose transferase to facilitate the transfer of substrate for callose synthesis.
This article has been cited by other articles:
|
|
|
|
 
D. A. Collings, L. K. Gebbie, P. A. Howles, U. A. Hurley, R. J. Birch, A. H. Cork, C. H. Hocart, T. Arioli, and R. E. Williamson
Arabidopsis dynamin-like protein DRP1A: a null mutant with widespread defects in endocytosis, cellulose synthesis, cytokinesis, and cell expansion
J. Exp. Bot.,
February 5, 2008;
(2008)
erm324v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. U. Furch, J. B. Hafke, A. Schulz, and A. J. E. van Bel
Ca2+-mediated remote control of reversible sieve tube occlusion in Vicia faba
J. Exp. Bot.,
August 1, 2007;
58(11):
2827 - 2838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Li, W. Xu, W. Yang, Z. Kong, and Y. Xue
Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice
Plant Physiology,
August 1, 2007;
144(4):
1797 - 1812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Geisler-Lee, M. Geisler, P. M. Coutinho, B. Segerman, N. Nishikubo, J. Takahashi, H. Aspeborg, S. Djerbi, E. Master, S. Andersson-Gunneras, et al.
Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses
Plant Physiology,
March 1, 2006;
140(3):
946 - 962.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Mahfouz, S. Kim, A. J. Delauney, and D. P. S. Verma
Arabidopsis TARGET OF RAPAMYCIN Interacts with RAPTOR, Which Regulates the Activity of S6 Kinase in Response to Osmotic Stress Signals
PLANT CELL,
February 1, 2006;
18(2):
477 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yasuhara
Caffeine Inhibits Callose Deposition in the Cell Plate and the Depolymerization of Microtubules in the Central Region of the Phragmoplast
Plant Cell Physiol.,
July 1, 2005;
46(7):
1083 - 1092.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-y. Yamada, S. Sonobe, and T. Shimmen
Synthesis of a Callosic Substance during Rhizoid Differentiation in Spirogyra
Plant Cell Physiol.,
November 15, 2003;
44(11):
1225 - 1228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Jacobs, V. Lipka, R. A. Burton, R. Panstruga, N. Strizhov, P. Schulze-Lefert, and G. B. Fincher
An Arabidopsis Callose Synthase, GSL5, Is Required for Wound and Papillary Callose Formation
PLANT CELL,
November 1, 2003;
15(11):
2503 - 2513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-H. Kang, J. S. Busse, and S. Y. Bednarek
Members of the Arabidopsis Dynamin-Like Gene Family, ADL1, Are Essential for Plant Cytokinesis and Polarized Cell Growth
PLANT CELL,
April 1, 2003;
15(4):
899 - 913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Porchia, S. O. Sorensen, and H. V. Scheller
Arabinoxylan Biosynthesis in Wheat. Characterization of Arabinosyltransferase Activity in Golgi Membranes
Plant Physiology,
September 1, 2002;
130(1):
432 - 441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. G. Cortes, J. Ishiguro, A. Duran, and J. C. Ribas
Localization of the (1,3){beta}-D-glucan synthase catalytic subunit homologue Bgs1p/Cps1p from fission yeast suggests that it is involved in septation, polarized growth, mating, spore wall formation and spore germination
J. Cell Sci.,
January 11, 2002;
115(21):
4081 - 4096.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Otegui, D. N. Mastronarde, B.-H. Kang, S. Y. Bednarek, and L. A. Staehelin
Three-Dimensional Analysis of Syncytial-Type Cell Plates during Endosperm Cellularization Visualized by High Resolution Electron Tomography
PLANT CELL,
September 1, 2001;
13(9):
2033 - 2051.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Hong, Z. Zhang, J. M. Olson, and D. P. S. Verma
A Novel UDP-Glucose Transferase Is Part of the Callose Synthase Complex and Interacts with Phragmoplastin at the Forming Cell Plate
PLANT CELL,
April 1, 2001;
13(4):
769 - 780.
[Abstract]
[Full Text]
|
|
|
|
|
