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Plant Cell, Vol. 13, 843-852, April 2001, Copyright © 2001, American Society of Plant Physiologists

Auxin Transport Promotes Arabidopsis Lateral Root Initiation

Ilda Casimiroa, Alan Marchantb, Rishikesh P. Bhaleraoc, Tom Beeckmand, Sandra Dhooged, Ranjan Swarupb, Neil Grahamb, Dirk Inzéd, Goran Sandbergc, Pedro J. Caseroa, and Malcolm Bennettb
a Departmento de Ciencias Morfologicas y Biologia Celular y Animal, University of Extremadura, Badajoz, Spain
b School of Biosciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom
c Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umea, Sweden
d Department of Genetics, Universiteit Gent, B-9000 Gent, Belgium

Correspondence to: Malcolm Bennett, malcolm.bennett{at}nottingham.ac.uk (E-mail), 44-115-9513298 (fax)

Lateral root development in Arabidopsis provides a model for the study of hormonal signals that regulate postembryonic organogenesis in higher plants. Lateral roots originate from pairs of pericycle cells, in several cell files positioned opposite the xylem pole, that initiate a series of asymmetric, transverse divisions. The auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) arrests lateral root development by blocking the first transverse division(s). We investigated the basis of NPA action by using a cell-specific reporter to demonstrate that xylem pole pericycle cells retain their identity in the presence of the auxin transport inhibitor. However, NPA causes indoleacetic acid (IAA) to accumulate in the root apex while reducing levels in basal tissues critical for lateral root initiation. This pattern of IAA redistribution is consistent with NPA blocking basipetal IAA movement from the root tip. Characterization of lateral root development in the shoot meristemless1 mutant demonstrates that root basipetal and leaf acropetal auxin transport activities are required during the initiation and emergence phases, respectively, of lateral root development.




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