Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (46)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Provencher, L. M.
Right arrow Articles by Lucas, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Provencher, L. M.
Right arrow Articles by Lucas, W. J.
Agricola
Right arrow Articles by Provencher, L. M.
Right arrow Articles by Lucas, W. J.
Plant Cell, Vol. 13, 1127-1141, May 2001, Copyright © 2001, American Society of Plant Physiologists

Sucrose Export Defective1 Encodes a Novel Protein Implicated in Chloroplast-to-Nucleus Signaling

Laurel Mezitt Provencher, Long Miao, Neelima Sinha, and William J. Lucas
Section of Plant Biology, Division of Biological Sciences, University of California, One Shields Avenue, Davis, California 95616

Correspondence to: William J. Lucas, wjlucas{at}ucdavis.edu (E-mail), 530-752-5410 (fax)

The Sucrose export defective1 (Sxd1) gene of maize was cloned and shown to encode a novel protein conserved between plants and cyanobacteria. The structure of the Sxd1 locus was determined in wild-type plants and two independent sxd1 alleles. Expression analysis demonstrated that the gene was transcribed in all green tissues, with highest levels in maturing leaf blades. In situ hybridization studies revealed high levels of Sxd1 mRNA in bundle sheath cells, with lower levels within the mesophyll. The SXD1 protein was localized to chloroplasts, in both bundle sheath and mesophyll cells. Levels of sucrose, glucose, and fructose were compared between wild-type and sxd1 plants. Mutant plants were fully capable of producing sucrose and accumulated all three sugars at concentrations above those measured in wild-type plants. Despite these increased sugar concentrations, photosynthetic gene expression was not significantly downregulated in affected areas of sxd1 leaf blades. These results are consistent with photosynthate being trapped within anthocyanin-accumulating regions of sxd1 leaves due to plasmodesmal occlusion at the bundle sheath–vascular parenchyma boundary of the minor veins. A model for SXD1 function is proposed in which the protein is involved in a chloroplast-to-nucleus signaling pathway necessary for proper late-stage differentiation of maize bundle sheath cells, including the developmentally regulated modification of plasmodesmata.




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
A. Sirikhachornkit, J. W. Shin, I. Baroli, and K. K. Niyogi
Replacement of {alpha}-Tocopherol by {beta}-Tocopherol Enhances Resistance to Photooxidative Stress in a Xanthophyll-Deficient Strain of Chlamydomonas reinhardtii
Eukaryot. Cell, November 1, 2009; 8(11): 1648 - 1657.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Stonebloom, T. Burch-Smith, I. Kim, D. Meinke, M. Mindrinos, and P. Zambryski
Loss of the plant DEAD-box protein ISE1 leads to defective mitochondria and increased cell-to-cell transport via plasmodesmata
PNAS, October 6, 2009; 106(40): 17229 - 17234.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. M. Braun and T. L. Slewinski
Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading
Plant Physiology, January 1, 2009; 149(1): 71 - 81.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Ma, T. L. Slewinski, R. F. Baker, and D. M. Braun
Tie-dyed1 Encodes a Novel, Phloem-Expressed Transmembrane Protein That Functions in Carbohydrate Partitioning
Plant Physiology, January 1, 2009; 149(1): 181 - 194.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. F. Baker and D. M. Braun
Tie-dyed2 Functions with Tie-dyed1 to Promote Carbohydrate Export from Maize Leaves
Plant Physiology, March 1, 2008; 146(3): 1085 - 1097.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Maeda, T. L. Sage, G. Isaac, R. Welti, and D. DellaPenna
Tocopherols Modulate Extraplastidic Polyunsaturated Fatty Acid Metabolism in Arabidopsis at Low Temperature
PLANT CELL, February 1, 2008; 20(2): 452 - 470.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A.-R. Abbasi, M. Hajirezaei, D. Hofius, U. Sonnewald, and L. M. Voll
Specific Roles of {alpha}- and {gamma}-Tocopherol in Abiotic Stress Responses of Transgenic Tobacco
Plant Physiology, April 1, 2007; 143(4): 1720 - 1738.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. M. Braun, Y. Ma, N. Inada, M. G. Muszynski, and R. F. Baker
tie-dyed1 Regulates Carbohydrate Accumulation in Maize Leaves
Plant Physiology, December 1, 2006; 142(4): 1511 - 1522.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Maeda, W. Song, T. L. Sage, and D. DellaPenna
Tocopherols Play a Crucial Role in Low-Temperature Adaptation and Phloem Loading in Arabidopsis
PLANT CELL, October 1, 2006; 18(10): 2710 - 2732.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Sakuragi, H. Maeda, D. DellaPenna, and D. A. Bryant
{alpha}-Tocopherol Plays a Role in Photosynthesis and Macronutrient Homeostasis of the Cyanobacterium Synechocystis sp. PCC 6803 That Is Independent of Its Antioxidant Function
Plant Physiology, June 1, 2006; 141(2): 508 - 521.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P.-A. Vidi, M. Kanwischer, S. Baginsky, J. R. Austin, G. Csucs, P. Dormann, F. Kessler, and C. Brehelin
Tocopherol Cyclase (VTE1) Localization and Vitamin E Accumulation in Chloroplast Plastoglobule Lipoprotein Particles
J. Biol. Chem., April 21, 2006; 281(16): 11225 - 11234.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Kanwischer, S. Porfirova, E. Bergmuller, and P. Dormann
Alterations in Tocopherol Cyclase Activity in Transgenic and Mutant Plants of Arabidopsis Affect Tocopherol Content, Tocopherol Composition, and Oxidative Stress
Plant Physiology, February 1, 2005; 137(2): 713 - 723.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Hofius, M.-R. Hajirezaei, M. Geiger, H. Tschiersch, M. Melzer, and U. Sonnewald
RNAi-Mediated Tocopherol Deficiency Impairs Photoassimilate Export in Transgenic Potato Plants
Plant Physiology, July 1, 2004; 135(3): 1256 - 1268.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. E. Sattler, L. U. Gilliland, M. Magallanes-Lundback, M. Pollard, and D. DellaPenna
Vitamin E Is Essential for Seed Longevity and for Preventing Lipid Peroxidation during Germination
PLANT CELL, June 1, 2004; 16(6): 1419 - 1432.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. L. Van Eenennaam, K. Lincoln, T. P. Durrett, H. E. Valentin, C. K. Shewmaker, G. M. Thorne, J. Jiang, S. R. Baszis, C. K. Levering, E. D. Aasen, et al.
Engineering Vitamin E Content: From Arabidopsis Mutant to Soy Oil
PLANT CELL, December 1, 2003; 15(12): 3007 - 3019.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Vitamin E-Defective Mutants of Arabidopsis Tell Tales of Convergent Evolution
PLANT CELL, October 1, 2003; 15(10): 2233 - 2235.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. E. Sattler, E. B. Cahoon, S. J. Coughlan, and D. DellaPenna
Characterization of Tocopherol Cyclases from Higher Plants and Cyanobacteria. Evolutionary Implications for Tocopherol Synthesis and Function
Plant Physiology, August 1, 2003; 132(4): 2184 - 2195.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Porfirova, E. Bergmuller, S. Tropf, R. Lemke, and P. Dormann
Isolation of an Arabidopsis mutant lacking vitamin E and identification of a cyclase essential for all tocopherol biosynthesis
PNAS, September 17, 2002; 99(19): 12495 - 12500.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
I. Kim, F. D. Hempel, K. Sha, J. Pfluger, and P. C. Zambryski
Identification of a developmental transition in plasmodesmatal function during embryogenesis in Arabidopsis thaliana
Development, January 3, 2002; 129(5): 1261 - 1272.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2001 by the American Society of Plant Biologists