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The Plant Cell, Vol. 13, 1347-1368, June 2001, Copyright © 2001,
American Society of Plant Physiologists

The Chloroplast Gene ycf9 Encodes a Photosystem II (PSII) Core Subunit, PsbZ, That Participates in PSII Supramolecular Architecture

Magdalena Swiatek1,a, Richard Kuras1,b, Anna Sokolenko1,a, David Higgs2,c, Jacqueline Olived, Gianfelice Cinquee, Bernd Müllera, Lutz A. Eichackera, David B. Sternc, Roberto Bassie, Reinhold G. Herrmanna and Francis-André Wollman3b

a Botanisches Institut der Ludwig-Maximilians-Universität, Menzingerstrasse 67, D-80638 Munich, Germany
b Unité Propre de Recherche–Centre National de la Recherche Scientifique 1261, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
c Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853
d Institut Jacques Monod, Université Paris 7, 2 Place Jussieu, 75251 Paris, France
e Dipartimento Scientifico e Tecnologico, Università di Verona, Strada Le Grazie, 37134 Verona, Italy

3 To whom correspondence should be addressed. E-mail wollman{at}ibpc.fr; fax 33-1-58-41-50-22

We have characterized the biochemical nature and the function of PsbZ, the protein product of a ubiquitous open reading frame, which is known as ycf9 in Chlamydomonas and ORF 62 in tobacco, that is present in chloroplast and cyanobacterial genomes. After raising specific antibodies to PsbZ from Chlamydomonas and tobacco, we demonstrated that it is a bona fide photosystem II (PSII) subunit. PsbZ copurifies with PSII cores in Chlamydomonas as well as in tobacco. Accordingly, PSII mutants from Chlamydomonas and tobacco are deficient in PsbZ. Using psbZ-targeted gene inactivation in tobacco and Chlamydomonas, we show that this protein controls the interaction of PSII cores with the light-harvesting antenna; in particular, PSII-LHCII supercomplexes no longer could be isolated from PsbZ-deficient tobacco plants. The content of the minor chlorophyll binding protein CP26, and to a lesser extent that of CP29, also was altered substantially under most growth conditions in the tobacco mutant and in Chlamydomonas mutant cells grown under photoautotrophic conditions. These PsbZ-dependent changes in the supramolecular organization of the PSII cores with their peripheral antennas cause two distinct phenotypes in tobacco and are accompanied by considerable modifications in (1) the pattern of protein phosphorylation within PSII units, (2) the deepoxidation of xanthophylls, and (3) the kinetics and amplitude of nonphotochemical quenching. The role of PsbZ in excitation energy dissipation within PSII is discussed in light of its proximity to CP43, in agreement with the most recent structural data on PSII.




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