Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (102)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peck, S. C.
Right arrow Articles by Boller, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peck, S. C.
Right arrow Articles by Boller, T.
Agricola
Right arrow Articles by Peck, S. C.
Right arrow Articles by Boller, T.
The Plant Cell, Vol. 13, 1467-1475, June 2001, Copyright © 2001,
American Society of Plant Physiologists

Directed Proteomics Identifies a Plant-Specific Protein Rapidly Phosphorylated in Response to Bacterial and Fungal Elicitors

Scott C. Peck13, Thomas S. Nühse, Daniel Hess, Alejandro Iglesias2, Fred Meins and Thomas Boller

Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland

3 To whom correspondence should be addressed. E-mail scott.peck{at}bbsrc.ac.uk; fax 44-01603-450011

The perception of microbial signal molecules is part of the strategy evolved by plants to survive attacks by potential pathogens. To gain a more complete understanding of the early signaling events involved in these responses, we used radioactive orthophosphate to pulse-label suspension-cultured cells of Arabidopsis in conjunction with two-dimensional gel electrophoresis and mass spectrometry to identify proteins that are phosphorylated rapidly in response to bacterial and fungal elicitors. One of these proteins, AtPhos43, and related proteins in tomato and rice, are phosphorylated within minutes after treatment with flagellin or chitin fragments. By measuring 32P incorporation into AtPhos43 immunoprecipitated from extracts of elicitor-treated hormone and defense-response mutants, we found that phosphorylation of AtPhos43 after flagellin treatment but not chitin treatment is dependent on FLS2, a receptor-like kinase involved in flagellin perception. Induction by both elicitors is not dependent on salicylic acid or EDS1, a putative lipase involved in defense signaling.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Aki, M. Shigyo, R. Nakano, T. Yoneyama, and S. Yanagisawa
Nano Scale Proteomics Revealed the Presence of Regulatory Proteins Including Three FT-Like proteins in Phloem and Xylem Saps from Rice
Plant Cell Physiol., May 1, 2008; 49(5): 767 - 790.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Merkouropoulos, E. Andreasson, D. Hess, T. Boller, and S. C. Peck
An Arabidopsis Protein Phosphorylated in Response to Microbial Elicitation, AtPHOS32, Is a Substrate of MAP Kinases 3 and 6
J. Biol. Chem., April 18, 2008; 283(16): 10493 - 10499.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Wan, X.-C. Zhang, D. Neece, K. M. Ramonell, S. Clough, S.-y. Kim, M. G. Stacey, and G. Stacey
A LysM Receptor-Like Kinase Plays a Critical Role in Chitin Signaling and Fungal Resistance in Arabidopsis
PLANT CELL, February 1, 2008; 20(2): 471 - 481.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
Z. Deng, X. Zhang, W. Tang, J. A. Oses-Prieto, N. Suzuki, J. M. Gendron, H. Chen, S. Guan, R. J. Chalkley, T. K. Peterman, et al.
A Proteomics Study of Brassinosteroid Response in Arabidopsis
Mol. Cell. Proteomics, December 1, 2007; 6(12): 2058 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. A. Gust, R. Biswas, H. D. Lenz, T. Rauhut, S. Ranf, B. Kemmerling, F. Gotz, E. Glawischnig, J. Lee, G. Felix, et al.
Bacteria-derived Peptidoglycans Constitute Pathogen-associated Molecular Patterns Triggering Innate Immunity in Arabidopsis
J. Biol. Chem., November 2, 2007; 282(44): 32338 - 32348.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Takahashi, G. K. Agrawal, M. Yamazaki, K. Onosato, A. Miyao, T. Kawasaki, K. Shimamoto, and H. Hirochika
Rice Pti1a Negatively Regulates RAR1-Dependent Defense Responses
PLANT CELL, September 1, 2007; 19(9): 2940 - 2951.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Heese, D. R. Hann, S. Gimenez-Ibanez, A. M. E. Jones, K. He, J. Li, J. I. Schroeder, S. C. Peck, and J. P. Rathjen
The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants
PNAS, July 17, 2007; 104(29): 12217 - 12222.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. J. Benschop, S. Mohammed, M. O'Flaherty, A. J. R. Heck, M. Slijper, and F. L. H. Menke
Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis
Mol. Cell. Proteomics, July 1, 2007; 6(7): 1198 - 1214.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
U. Roth, E. von Roepenack-Lahaye, and S. Clemens
Proteome changes in Arabidopsis thaliana roots upon exposure to Cd2+
J. Exp. Bot., December 1, 2006; 57(15): 4003 - 4013.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. M.E. Jones, V. Thomas, M. H. Bennett, J. Mansfield, and M. Grant
Modifications to the Arabidopsis Defense Proteome Occur Prior to Significant Transcriptional Change in Response to Inoculation with Pseudomonas syringae
Plant Physiology, December 1, 2006; 142(4): 1603 - 1620.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. C Peck
Phosphoproteomics in Arabidopsis: moving from empirical to predictive science
J. Exp. Bot., April 1, 2006; 57(7): 1523 - 1527.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. J. Kwon, E. Y. Choi, Y. J. Choi, J. H. Ahn, and O. K Park
Proteomics studies of post-translational modifications in plants
J. Exp. Bot., April 1, 2006; 57(7): 1547 - 1551.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Fujiwara, K. Umemura, T. Kawasaki, and K. Shimamoto
Proteomics of Rac GTPase Signaling Reveals Its Predominant Role in Elicitor-Induced Defense Response of Cultured Rice Cells
Plant Physiology, February 1, 2006; 140(2): 734 - 745.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Katou, H. Yoshioka, K. Kawakita, O. Rowland, J. D.G. Jones, H. Mori, and N. Doke
Involvement of PPS3 Phosphorylated by Elicitor-Responsive Mitogen-Activated Protein Kinases in the Regulation of Plant Cell Death
Plant Physiology, December 1, 2005; 139(4): 1914 - 1926.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Heese, A. A. Ludwig, and J. D.G. Jones
Rapid Phosphorylation of a Syntaxin during the Avr9/Cf-9-Race-Specific Signaling Pathway
Plant Physiology, August 1, 2005; 138(4): 2406 - 2416.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I.-P. Ahn, S. Kim, and Y.-H. Lee
Vitamin B1 Functions as an Activator of Plant Disease Resistance
Plant Physiology, July 1, 2005; 138(3): 1505 - 1515.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. C. Peck
Update on Proteomics in Arabidopsis. Where Do We Go From Here?
Plant Physiology, June 1, 2005; 138(2): 591 - 599.
[Full Text] [PDF]

Home page
 Plant CellHome page
T. S. Nuhse, A. Stensballe, O. N. Jensen, and S. C. Peck
Phosphoproteomics of the Arabidopsis Plasma Membrane and a New Phosphorylation Site Database
PLANT CELL, September 1, 2004; 16(9): 2394 - 2405.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. S. Nuhse, T. Boller, and S. C. Peck
A Plasma Membrane Syntaxin Is Phosphorylated in Response to the Bacterial Elicitor Flagellin
J. Biol. Chem., November 14, 2003; 278(46): 45248 - 45254.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
T. S. Nuhse, A. Stensballe, O. N. Jensen, and S. C. Peck
Large-scale Analysis of in Vivo Phosphorylated Membrane Proteins by Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry
Mol. Cell. Proteomics, November 1, 2003; 2(11): 1234 - 1243.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y.-Q. Zhu, K.-X. Xu, B. Luo, J.-W. Wang, and X.-Y. Chen
An ATP-Binding Cassette Transporter GhWBC1 from Elongating Cotton Fibers
Plant Physiology, October 1, 2003; 133(2): 580 - 588.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Kuhlmann, K. Horvay, A. Strathmann, T. Heinekamp, U. Fischer, S. Bottner, and W. Droge-Laser
The alpha -Helical D1 Domain of the Tobacco bZIP Transcription Factor BZI-1 Interacts with the Ankyrin-repeat Protein ANK1 and Is Important for BZI-1 Function, Both in Auxin Signaling and Pathogen Response
J. Biol. Chem., February 28, 2003; 278(10): 8786 - 8794.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Finnie, S. Melchior, P. Roepstorff, and B. Svensson
Proteome Analysis of Grain Filling and Seed Maturation in Barley
Plant Physiology, July 1, 2002; 129(3): 1308 - 1319.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. Kruger, C. M. Thomas, C. Golstein, M. S. Dixon, M. Smoker, S. Tang, L. Mulder, and J. D. G. Jones
A Tomato Cysteine Protease Required for Cf-2-Dependent Disease Resistance and Suppression of Autonecrosis
Science, April 26, 2002; 296(5568): 744 - 747.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2001 by the American Society of Plant Biologists